Arginine/agmatine antiporter (P60061)

Identification
Name:Arginine/agmatine antiporter
Synonyms:Not Available
Gene Name:adiC
Enzyme Class:Not Available
Biological Properties
General Function:Involved in amino acid transmembrane transporter activity
Specific Function:Major component of the acid-resistance (AR) system allowing enteric pathogens to survive the acidic environment in the stomach. Exchanges extracellular arginine for its intracellular decarboxylation product agmatine (Agm) thereby expelling intracellular protons
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB01432AgmatineMetaboCard
ECMDB00517L-ArginineMetaboCard
GO Classification:
Component
cell part
membrane
Function
active transmembrane transporter activity
amine transmembrane transporter activity
amino acid transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
amine transport
amino acid transport
establishment of localization
transmembrane transport
transport
Gene Properties
Blattner:b4115
Gene OrientationCounterclockwise
Centisome Percentage:93.41
Left Sequence End4333717
Right Sequence End4335054
Gene Sequence:
>1338 bp
ATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTG
ACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTG
GGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGC
GGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATG
GAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCA
GTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTG
GGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTA
TTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCA
CATCCGGCTGCTTTATATGTTGCCAATAAGCTGGGCGCAGATATCGGTTCGGTGATCGTC
TACGGTTTGCTGGTTGGGCTGATGGCATCACTGATCGGTGGCCCACTTTTCCTTAAATTT
CTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGC
GATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATACTGCTACCCATTGCG
CTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATC
TTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCGTGTTTGTCGCCTAT
TATGTGTTGGGTATACGCCAGCATATGAGCATGGGGACGATGCTCACACATACGGAAAAT
GGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCC
ATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATG
CACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCT
ACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGAC
ATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTT
ACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTT
AAATACTATACGACAGCGACATTTATCGCTTCAGTCGTCGCTCTGGCGGGCACATTCCTG
CTGTCATTTATCATCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:445
Protein Molecular Weight:46842
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 10-30
  • 39-59
  • 99-119
  • 123-143
  • 153-173
  • 197-217
  • 226-246
  • 276-296
  • 322-342
  • 356-376
  • 386-406
  • 409-429
Protein Sequence:
>Arginine/agmatine antiporter
MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIYGWLVTIIGALGLSMVY
AKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNIAMVVIGVGYLSYFFPILK
DPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALIPIVGIAVFGWFWFRGETYMA
AWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVKNPKRNVPIATIGGVLIAAVCYV
LSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGAIVSFCAAAGCLGSLGGWTLLAGQT
AKAAADDGLFPPIFARVNKAGTPVAGLIIVGILMTIFQLSSISPNATKEFGLVSSVSVIF
TLVPYLYTCAALLLLGHGHFGKARPAYLAVTTIAFLYCIWAVVGSGAKEVMWSFVTLMVI
TAMYALNYNRLHKNPYPLDAPISKD
References
External Links:
ResourceLink
Uniprot ID:P60061
Uniprot Name:ADIC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1799764
Ecogene ID:EG12462
Ecocyc:EG12462
ColiBase:b4115
Kegg Gene:b4115
EchoBASE ID:EB2355
CCDB:ADIC_ECOLI
BacMap:16131941
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Gong, S., Richard, H., Foster, J. W. (2003). "YjdE (AdiC) is the arginine:agmatine antiporter essential for arginine-dependent acid resistance in Escherichia coli." J Bacteriol 185:4402-4409. Pubmed: 12867448
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Iyer, R., Williams, C., Miller, C. (2003). "Arginine-agmatine antiporter in extreme acid resistance in Escherichia coli." J Bacteriol 185:6556-6561. Pubmed: 14594828