ECMDB

Identification

Name:

Putative carboxymethylenebutenolidase

Synonyms:

Dienelactone hydrolase
DLH

Gene Name:

ysgA

Enzyme Class:

3.1.1.45

Biological Properties

General Function:

Involved in hydrolase activity

Specific Function:

4-carboxymethylenebut-2-en-4-olide + H(2)O = 4-oxohex-2-enedioate

Cellular Location:

Cytoplasmic

SMPDB Pathways:

Collection of Reactions without pathways PW001891
Fluorobenzoate degradation PW000766

KEGG Pathways:

1,4-Dichlorobenzene degradation ec00627
Fluorobenzoate degradation ec00364
Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Toluene degradation ec00623
gamma-Hexachlorocyclohexane degradation ec00361

KEGG Reactions:

1.0

↔

1.0

1.0Cis-4-Carboxymethylenebut-2-en-4-olide + 1.0Water ↔ 1.02-Maleylacetate
ReactionCard

1.0

↔

1.0

1.02,5-Dichloro-carboxymethylenebut-2-en-4-olide + 1.0Water ↔ 1.02,5-Dichloro-4-oxohex-2-enedioate
ReactionCard

1.0

↔

1.0

1.0Trans-4-Carboxymethylenebut-2-en-4-olide + 1.0Water ↔ 1.02-Maleylacetate
ReactionCard

1.05-Fluoromuconolactone

1.0

↔

1.0

1.0Hydrofluoric acid

1.05-Fluoromuconolactone + 1.0Water ↔ 1.02-Maleylacetate + 1.0Hydrofluoric acid
ReactionCard

1.04-Fluoromuconolactone

1.0

↔

1.0

1.0Hydrofluoric acid

1.04-Fluoromuconolactone + 1.0Water ↔ 1.02-Maleylacetate + 1.0Hydrofluoric acid
ReactionCard

1.02,3,5-Trichlorodienelactone

1.0

↔

1.02,3,5-Trichloromaleylacetate

1.02,3,5-Trichlorodienelactone + 1.0Water ↔ 1.02,3,5-Trichloromaleylacetate
ReactionCard

1.03-Chloro-2-methyldienelactone

1.0

↔

1.03-Chloro-2-methylmaleylacetate

1.03-Chloro-2-methyldienelactone + 1.0Water ↔ 1.03-Chloro-2-methylmaleylacetate
ReactionCard

1.02-Chloro-5-methyl-cis-dienelactone

1.0

↔

1.02-Chloro-5-methylmaleylacetate

1.02-Chloro-5-methyl-cis-dienelactone + 1.0Water ↔ 1.02-Chloro-5-methylmaleylacetate
ReactionCard

SMPDB Reactions:

1.0Hydrofluoric acid

1.0

→

1.0

1.0hydrofluoric acid

1.0Hydrofluoric acid + 1.0Water → 1.02-Maleylacetate + 1.0hydrofluoric acid
ReactionCard

1.05-Fluoromuconolactone

1.0

→

1.0

1.0hydrofluoric acid

1.05-Fluoromuconolactone + 1.0Water → 1.02-Maleylacetate + 1.0hydrofluoric acid
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Cis-4-Carboxymethylenebut-2-en-4-olide + 1.0Water → 1.04-Oxohex-2-enedioate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB20060	2,5-Dichloro-4-oxohex-2-enedioate	MetaboCard
ECMDB20061	2,5-Dichloro-carboxymethylenebut-2-en-4-olide	MetaboCard
ECMDB20045	2-Maleylacetate	MetaboCard
ECMDB23058	4-Oxohex-2-enedioate	MetaboCard
ECMDB20128	Cis-2-Chloro-4-carboxymethylenebut-2-en-1,4-olide	MetaboCard
ECMDB20131	Cis-4-Carboxymethylenebut-2-en-4-olide	MetaboCard
ECMDB20132	cis-Acetylacrylate	MetaboCard
ECMDB20198	Trans-4-Carboxymethylenebut-2-en-4-olide	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
hydrolase activity

Gene Properties

Blattner:

b3830

Gene Orientation

Counterclockwise

Centisome Percentage:

86.50

Left Sequence End

4013377

Right Sequence End

4014192

Gene Sequence:

>816 bp
ATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTT
TGGGGCGTAGTGTTAGGCCTGTTAATTGGCCATATGTTTGATAAAGCCCGTAGCCGTAAA
ATGGCGTGGTTCGCCAACCAGCGTGAGCGTCAGGCGCTGTTTTTTGCCACCACTTTTGAA
GTGATGGGGCATTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCC
AGCCAGTTGATGGACCGAATGAATCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCG
TTCCGGGTGGGAAAATCAGACAATTACCCGCTGCGCGAAAAGATGCGCCAGTTTCGCAGT
GTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCG
TTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCATTGCAGAAGAA
TTAGGGATCTCCCGCGCTCAGTTTGACCAGTTTTTGCGCATGATGCAGGGCGGTGCACAG
TTTGGCGGCGGTTATCAGCAGCAAACTGGCGGTGGTAACTGGCAGCAAGCGCAGCGTGGC
CCAACGCTGGAAGATGCCTGTAATGTGCTGGGCGTGAAGCCGACGGATGATGCGACCACC
ATCAAACGTGCCTACCGTAAGCTGATGAGTGAACACCATCCCGATAAGCTGGTGGCGAAA
GGTTTGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCA
TATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGA

Protein Properties

Pfam Domain Function:

DLH (PF01738 )

Protein Residues:

271

Protein Molecular Weight:

29425

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Putative carboxymethylenebutenolidase
MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPAYHARPKQSDGPLPVVI
VVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPDSQV
LADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHNPQLKAAVAWYGKLTGDKSLN
SPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQALRAANAKAEIIVYPDAGHAFNA
DYRPSYHAASAEDGWQRMLEWFKQYGGKKSL

References

External Links:

Resource	Link
Uniprot ID:	P56262
Uniprot Name:	DLHH_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	21321936
Ecogene ID:	EG14321
Ecocyc:	EG14321
ColiBase:	b3830
Kegg Gene:	b3830
EchoBASE ID:	EB4067
CCDB:	DLHH_ECOLI
BacMap:	49176425

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293

Putative carboxymethylenebutenolidase (P56262)