| Identification |
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| Name: | Endonuclease 8 |
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| Synonyms: | Not Available |
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| Gene Name: | nei |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | nucleotide-excision repair |
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| Specific Function: | Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Acts on DNA bubble and 3'-fork structures, suggesting a role in replication-associated DNA repair. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. |
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| Cellular Location: | Not Available |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | |
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| Metabolites: | |
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| GO Classification: | | Function |
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| base-excision repair | | damaged DNA binding | | DNA-(apurinic or apyrimidinic site) lyase activity | | nucleotide-excision repair | | oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity | | zinc ion binding |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >792
atgcctgaaggcccggagatccgccgtgcagcggataacctggaggcggcgatcaaaggc
aaaccactaactgatgtctggtttgccttcccgcagttaaaaccttatcaatcacaactt
atcggtcaacacgttacccatgtggaaacgcgtggtaaggcgttgttaactcatttttcc
aacgacttaacgctctacagccataatcagctttacggcgtctggcgcgtggttgatacc
ggcgaagagccgcagaccacgcgagtattgcgggtaaaactgcaaacggctgacaaaacc
attctgctttatagcgcctcggatattgagatgttgaccccggaacaactgaccacgcat
ccgtttttacaacgcgttggtcccgatgtgctggatccgaatctgacgccggaggtggtg
aaagaacgattattgtcgccgcgctttcgtaaccgtcagtttgctggattactgctcgat
caggcgtttctggctgggcttggcaattatttgcgggtggagatcctctggcaggttggg
ttgactggaaatcataaagcgaaagatctcaatgcggcgcaactggatgcactcgcacac
gcgttactggagattcctcgattttcctacgctacgcgggggcaggtggatgagaataag
catcatggggcgctgtttcgctttaaggtttttcatcgagatggcgaaccgtgcgaacgt
tgtggcagcatcattgagaaaaccacgctgtcatctcgcccgttttactggtgccctggc
tgccagcactag |
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| Protein Properties |
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| Pfam Domain Function: | Not Available |
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| Protein Residues: | 263 |
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| Protein Molecular Weight: | 29844 |
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| Protein Theoretical pI: | Not Available |
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| PDB File: | 1K3W |
| Signaling Regions: | Not Available |
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| Transmembrane Regions: | Not Available |
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| Protein Sequence: | >Endonuclease 8
MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKPYQSQLIGQHVTHVETRGKALLTHFS
NDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTILLYSASDIEMLTPEQLTTH
PFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLDQAFLAGLGNYLRVEILWQVG
LTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDENKHHGALFRFKVFHRDGEPCER
CGSIIEKTTLSSRPFYWCPGCQH |
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| References |
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| External Links: | |
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| General Reference: | Not Available |
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