RNA 3'-terminal phosphate cyclase (P46849)

Identification
Name:RNA 3'-terminal phosphate cyclase
Synonyms:
  • RNA cyclase
  • RNA-3'-phosphate cyclase
Gene Name:rtcA
Enzyme Class:
Biological Properties
General Function:Involved in RNA processing
Specific Function:Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0RNA 3'-terminal-phosphate ↔ 1.0Thumb+1.0Thumb+1.0RNA terminal-2',3'-cyclic-phosphate
1.0Adenosine triphosphate + 1.0RNA 3'-terminal-phosphate ↔ 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0RNA terminal-2',3'-cyclic-phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0RNA 3'-terminal-phosphate → 1.0Thumb+1.0Thumb+1.0RNA terminal-2',3'-cyclic-phosphate
1.0Adenosine triphosphate + 1.0RNA 3'-terminal-phosphate → 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0RNA terminal-2',3'-cyclic-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
cyclase activity
ligase activity
ligase activity, forming phosphoric ester bonds
RNA-3'-phosphate cyclase activity
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
RNA metabolic process
RNA processing
Gene Properties
Blattner:b4475
Gene OrientationCounterclockwise
Centisome Percentage:76.60
Left Sequence End3553855
Right Sequence End3554871
Gene Sequence:
>1017 bp
ATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCC
GACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATG
GAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCT
GGTAATGGCCGGCGACCTTATCCGCTGGAAACCATGCTACGCATTCACTGCATGCAGCAT
TGGTACAACCTGAGCGATGGCGCGATGGAAGATGCTCTGTACGAAATCGCCTCCATGCGT
CTGTTTGCCCGGTTATCCCTGGATAGCGCCTTGCCGGACCGCACCACCATCATGAATTTC
CGCCACCTGCTGGAGCAGCATCAACTGGCCCGCCAATTGTTCAAGACCATCAATCGCTGG
CTGGCCGAAGCAGGCGTCATGATGACTCAAGGCACCTTGGTCGATGCCACCATCATTGAG
GCACCCAGCTCGACCAAGAACAAAGAGCAGCAACGCGATCCGGAGATGCATCAGACCAAG
AAAGGCAATCAGTGGCACTTTGGCATGAAGGCCCACATTGGTGTCGATGCCAAGAGTGGC
CTGACCCACAGCCTGGTCACCACCGCGGCCAACGAGCATGACCTCAATCAGCTGGGTAAT
CTGCTGCATGGAGAGGAGCAATTTGTCTCAGCCGATGCCGGCTACCAAGGGGCGCCACAG
CGCGAGGAGCTGGCCGAGGTGGATGTGGACTGGCTGATCGCCGAGCGCCCCGGCAAGGTA
AGAACCTTGAAACAGCATCCACGCAAGAACAAAACGGCCATCAACATCGAATACATGAAA
GCCAGCATCCGGGCCAGGGTGGAGCACCCATTTCGCATCATCAAGCGACAGTTCGGCTTC
GTGAAAGCCAGATACAAGGGGTTGCTGAAAAACGATAACCAACTGGCGATGTTATTCACG
CTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:338
Protein Molecular Weight:35903
Protein Theoretical pI:7
PDB File:1QMH
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>RNA 3'-terminal phosphate cyclase
MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAKPGLLRQHLTAVKAATE
ICGATVEGAELGSQRLLFRPGTVRGGDYRFAIGSAGSCTLVLQTVLPALWFADGPSRVEV
SGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFYPAGGGVVATEVSPVASFNTL
QLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSLHEQNIHNLPRDQGPGNTVSLEV
ESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLASTAAVGEYLADQLVLPMALAGAGEF
TVAHPSCHLLTNIAVVERFLPVRFSLIETDGVTRVSIE
References
External Links:
ResourceLink
Uniprot ID:P46849
Uniprot Name:RTCA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742188
PDB ID:1QMH
Ecogene ID:EG12938
Ecocyc:EG12938
ColiBase:b4475
Kegg Gene:b4475
EchoBASE ID:EB2773
CCDB:RTCA_ECOLI
BacMap:49176352
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cole, S. T., Raibaud, O. (1986). "The nucleotide sequence of the malT gene encoding the positive regulator of the Escherichia coli maltose regulon." Gene 42:201-208. Pubmed: 3015733
  • Genschik, P., Billy, E., Swianiewicz, M., Filipowicz, W. (1997). "The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea." EMBO J 16:2955-2967. Pubmed: 9184239
  • Genschik, P., Drabikowski, K., Filipowicz, W. (1998). "Characterization of the Escherichia coli RNA 3'-terminal phosphate cyclase and its sigma54-regulated operon." J Biol Chem 273:25516-25526. Pubmed: 9738023
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Palm, G. J., Billy, E., Filipowicz, W., Wlodawer, A. (2000). "Crystal structure of RNA 3'-terminal phosphate cyclase, a ubiquitous enzyme with unusual topology." Structure 8:13-23. Pubmed: 10673421
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293