ECMDB: p-aminobenzoyl-glutamate transport protein (P46133)

Identification

Name:

p-aminobenzoyl-glutamate transport protein

Synonyms:

PABA-GLU transport protein

Gene Name:

abgT

Enzyme Class:

Not Available

Biological Properties

General Function:

Not Available

Specific Function:

Essential for aminobenzoyl-glutamate utilization. It catalyzes the concentration-dependent uptake of p-aminobenzoyl-glutamate (PABA-GLU) into the cell and allows accumulation of PABA-GLU to a concentration enabling AbgAB to catalyze cleavage into p-aminobenzoate and glutamate. It seems also to increase the sensitivity to low levels of aminobenzoyl-glutamate. May actually serve physiologically as a transporter for some other molecule, perhaps a dipeptide, and that it transports p-aminobenzoyl-glutamate as a secondary activity. The physiological role of abgABT should be clarified. Essential for aminobenzoyl-glutamate utilization. It catalyzes the concentration-dependent uptake of p-aminobenzoyl-glutamate (PABA-GLU) into the cell and allows accumulation of PABA-GLU to a concentration enabling AbgAB to catalyze cleavage into p-aminobenzoate and glutamate. It seems also to increase the sensitivity to low levels of aminobenzoyl-glutamate. May actually serve physiologically as a transporter for some other molecule, perhaps a dipeptide, and that it transports p-aminobenzoyl-glutamate as a secondary activity. The physiological role of abgABT should be clarified.

Cellular Location:

Not Available

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

Metabolites:

ECMDB ID	Name	View
ECMDB20520	p-Aminobenzoyl glutamate	MetaboCard

GO Classification:

Not Available

Gene Properties

Blattner:

b1336

Gene Orientation

Not Available

Centisome Percentage:

Not Available

Left Sequence End

Not Available

Right Sequence End

Not Available

Gene Sequence:

>ENA|AAC74418|AAC74418.2 Escherichia coli str. K-12 substr. MG1655 p-aminobenzoyl-glutamate transporter; membrane protein
ATGAGTATGTCATCCATACCGTCGTCCTCCCAATCCGGGAAGCTCTATGGCTGGGTCGAA
AGAATTGGTAACAAGGTTCCCCATCCTTTTCTGCTCTTTATCTATTTGATTATCGTACTC
ATGGTGACGACGGCAATTTTGTCGGCCTTTGGCGTCAGTGCGAAAAACCCGACCGATGGT
ACGCCGGTCGTGGTGAAAAACCTGCTCAGTGTGGAAGGATTACACTGGTTTTTACCCAAT
GTTATTAAAAACTTTAGCGGTTTTGCTCCACTTGGTGCGATCCTGGCGCTGGTTTTAGGT
GCCGGTCTGGCGGAGCGCGTCGGCTTACTGCCAGCACTAATGGTTAAAATGGCATCGCAT
GTTAATGCCCGCTACGCCAGTTATATGGTGCTGTTTATTGCTTTTTTCAGCCACATTTCT
TCCGATGCGGCGTTAGTGATCATGCCACCGATGGGTGCGCTGATTTTTCTGGCGGTGGGC
AGGCATCCAGTTGCAGGTTTACTGGCTGCCATTGCAGGCGTAGGTTGCGGCTTTACGGCT
AATTTACTGATTGTCACAACCGACGTGTTGCTGTCGGGGATCAGCACGGAAGCGGCAGCT
GCGTTCAATCCGCAAATGCACGTCAGTGTAATTGATAACTGGTATTTTATGGCCAGCTCC
GTAGTCGTACTGACGATTGTTGGCGGCCTGATAACCGACAAAATCATCGAGCCACGGTTA
GGTCAATGGCAGGGAAACAGCGATGAGAAACTGCAGACATTGACCGAAAGTCAGCGTTTT
GGTTTACGCATAGCAGGTGTCGTATCGCTACTTTTTATTGCTGCGATTGCGCTGATGGTG
ATCCCGCAAAACGGGATATTGCGCGATCCGATTAATCACACCGTGATGCCATCACCCTTT
ATTAAAGGTATCGTGCCACTGATCATTCTTTTTTTCTTTGTTGTCTCGCTGGCTTATGGC
ATCGCTACCCGCACAATTCGACGTCAGGCGGATTTACCGCATTTAATGATTGAACCGATG
AAAGAGATGGCGGGATTTATCGTGATGGTTTTTCCCCTCGCCCAATTTGTCGCCATGTTT
AACTGGAGCAACATGGGGAAATTCATCGCCGTGGGGCTGACCGATATACTGGAAAGTTCA
GGGCTTAGCGGCATCCCGGCGTTTGTCGGTCTGGCGTTGCTTTCCTCTTTCTTATGCATG
TTTATTGCCAGCGGTTCCGCAATCTGGTCGATTCTCGCCCCCATTTTCGTACCAATGTTT
ATGCTACTTGGCTTTCACCCGGCATTTGCGCAAATCCTCTTTCGTATTGCCGACTCATCC
GTATTGCCTTTAGCGCCGGTATCTCCTTTTGTTCCACTGTTTCTTGGATTCCTGCAACGC
TACAAACCAGACGCGAAACTGGGTACTTACTATTCGTTAGTCTTGCCCTATCCACTTATC
TTTTTGGTGGTATGGCTGCTGATGTTGCTGGCGTGGTATCTTGTCGGTCTGCCGATAGGT
CCGGGTATTTACCCACGTTTGTCTTAA

Protein Properties

Pfam Domain Function:

Not Available

Protein Residues:

Not Available

Protein Molecular Weight:

Not Available

Protein Theoretical pI:

Not Available

Signaling Regions:

Not Available

Transmembrane Regions:

Not Available

Protein Sequence:

>sp|P46133|ABGT_ECOLI p-aminobenzoyl-glutamate transport protein OS=Escherichia coli (strain K12) GN=abgT PE=1 SV=3
MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTTAILSAFGVSAKNPTDG
TPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAGLAERVGLLPALMVKMASH
VNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVGRHPVAGLLAAIAGVGCGFTA
NLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMASSVVVLTIVGGLITDKIIEPRL
GQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAIALMVIPQNGILRDPINHTVMPSPF
IKGIVPLIILFFFVVSLAYGIATRTIRRQADLPHLMIEPMKEMAGFIVMVFPLAQFVAMF
NWSNMGKFIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSILAPIFVPMF
MLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGTYYSLVLPYPLI
FLVVWLLMLLAWYLVGLPIGPGIYPRLS

References

External Links:

Resource	Link
Uniprot ID:	P46133
ColiBase:	b1336
Kegg Gene:	b1336
BacMap:	90111251

General Reference:

Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Borodovsky, M., McIninch, J. D., Koonin, E. V., Rudd, K. E., Medigue, C., Danchin, A. (1995). "Detection of new genes in a bacterial genome using Markov models for three gene classes." Nucleic Acids Res 23:3554-3562. Pubmed: 7567469
Carter, E. L., Jager, L., Gardner, L., Hall, C. C., Willis, S., Green, J. M. (2007). "Escherichia coli abg genes enable uptake and cleavage of the folate catabolite p-aminobenzoyl-glutamate." J Bacteriol 189:3329-3334. Pubmed: 17307853
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Green, J. M., Hollandsworth, R., Pitstick, L., Carter, E. L. (2010). "Purification and characterization of the folate catabolic enzyme p-aminobenzoyl-glutamate hydrolase from Escherichia coli." J Bacteriol 192:2407-2413. Pubmed: 20190044
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Hussein, M. J., Green, J. M., Nichols, B. P. (1998). "Characterization of mutations that allow p-aminobenzoyl-glutamate utilization by Escherichia coli." J Bacteriol 180:6260-6268. Pubmed: 9829935
Potter, P. M., Wilkinson, M. C., Fitton, J., Carr, F. J., Brennand, J., Cooper, D. P., Margison, G. P. (1987). "Characterisation and nucleotide sequence of ogt, the O6-alkylguanine-DNA-alkyltransferase gene of E. coli." Nucleic Acids Res 15:9177-9193. Pubmed: 2825131