Sulfurtransferase tusD (P45532)

Identification
Name:Sulfurtransferase tusD
Synonyms:
  • tRNA 2-thiouridine synthesizing protein D
Gene Name:tusD
Enzyme Class:Not Available
Biological Properties
General Function:Involved in sulfurtransferase activity
Specific Function:Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Accepts sulfur from tusA and transfers it in turn to tusE
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
sulfurtransferase activity
transferase activity
transferase activity, transferring sulfur-containing groups
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
tRNA metabolic process
tRNA processing
Gene Properties
Blattner:b3345
Gene OrientationCounterclockwise
Centisome Percentage:74.86
Left Sequence End3473354
Right Sequence End3473740
Gene Sequence:
>387 bp
ATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTG
GGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTG
TTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCC
GTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTC
AGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACT
TTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTC
CTGCCCACGACAAAACAACGTTACTGA
Protein Properties
Pfam Domain Function:
Protein Residues:128
Protein Molecular Weight:13641
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Sulfurtransferase tusD
MRFAIVVTGPAYGTQQASSAFQFAQALIADGHELSSVFFYREGVYNANQLTSPASDEFDL
VRAWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSNLQQGFTLSGLGALAEASL
TCDRVVQF
References
External Links:
ResourceLink
Uniprot ID:P45532
Uniprot Name:TUSD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675328
Ecogene ID:EG12898
Ecocyc:EG12898
ColiBase:b3345
Kegg Gene:b3345
EchoBASE ID:EB2735
CCDB:TUSD_ECOLI
BacMap:16131224
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ikeuchi, Y., Shigi, N., Kato, J., Nishimura, A., Suzuki, T. (2006). "Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions." Mol Cell 21:97-108. Pubmed: 16387657
  • Numata, T., Fukai, S., Ikeuchi, Y., Suzuki, T., Nureki, O. (2006). "Structural basis for sulfur relay to RNA mediated by heterohexameric TusBCD complex." Structure 14:357-366. Pubmed: 16472754