ECMDB: N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 1 (P42909)

Identification

Name:

N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 1

Synonyms:

EIIB-Aga
PTS system N-acetylgalactosamine-specific EIIB component 1

Gene Name:

agaB

Enzyme Class:

2.7.1.69

Biological Properties

General Function:

Involved in protein-N(PI)-phosphohistidine-sugar phosphotransferase activity

Specific Function:

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N-acetylgalactosamine transport

Cellular Location:

Cytoplasm (Potential)

SMPDB Pathways:

Galactose metabolism PW000821
Starch and sucrose metabolism PW000941

KEGG Pathways:

Amino sugar and nucleotide sugar metabolism ec00520
Ascorbate and aldarate metabolism ec00053
Fructose and mannose metabolism ec00051
Galactose metabolism ec00052
Glycolysis / Gluconeogenesis ec00010
Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Pentose phosphate pathway ec00030
Starch and sucrose metabolism ec00500

KEGG Reactions:

1.0

1.0Protein N(pi)-phospho-L-histidine

↔

1.0

1.0Protein histidine

1.0D-Galactosamine + 1.0Protein N(pi)-phospho-L-histidine ↔ 1.0D-Galactosamine 6-phosphate + 1.0Protein histidine
ReactionCard

1.0Protein N(pi)-phospho-L-histidine

1.0Sugar

↔

1.0Protein histidine

1.0

1.0Protein N(pi)-phospho-L-histidine + 1.0Sugar ↔ 1.0Protein histidine + 1.0Sugar phosphate
ReactionCard

Complex Reactions:

1.0Protein EIIB N(pi)-phospho-L-histidine/cysteine

1.0

→

1.0protein EIIB

1.0sugar phosphate

1.0Protein EIIB N(pi)-phospho-L-histidine/cysteine + 1.0Sucrose → 1.0protein EIIB + 1.0sugar phosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB20142	D-Galactosamine	MetaboCard
ECMDB20141	D-Galactosamine 6-phosphate	MetaboCard
ECMDB00258	Sucrose	MetaboCard

GO Classification:

Component
cell part
cytoplasm
intracellular part
Function
carbohydrate transmembrane transporter activity
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
establishment of localization
phosphoenolpyruvate-dependent sugar phosphotransferase system
transport

Gene Properties

Blattner:

b3138

Gene Orientation

Clockwise

Centisome Percentage:

70.74

Left Sequence End

3282192

Right Sequence End

3282668

Gene Sequence:

>477 bp
ATGACCAGTCCAAATATTCTCTTAACCCGTATTGATAACCGTCTGGTTCACGGTCAGGTT
GGCGTGACCTGGACATCCACCATCGGTGCAAATCTGCTGGTAGTCGTGGATGATGTTGTC
GCTAACGATGATATTCAACAGAAATTGATGGGTATTACCGCGGAAACCTACGGCTTTGGC
ATTCGTTTCTTTACTATCGAAAAAACCATTAACGTCATCGGCAAAGCTGCACCACATCAG
AAGATCTTCCTGATTTGCCGTACGCCACAAACGGTACGTAAATTGGTAGAAGGTGGTATT
GACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGC
AGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGC
GTGAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATCCCTGACTAA

Protein Properties

Pfam Domain Function:

PTSIIB_sorb (PF03830 )

Protein Residues:

158

Protein Molecular Weight:

17622

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 1
MTSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDDIQQKLMGITAETYGFG
IRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDLKDVNVGNMHFSEGKKQIS
SKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD

References

External Links:

Resource	Link
Uniprot ID:	P42909
Uniprot Name:	PTPB1_ECOLI
GenBank Gene ID:	AF228498
Genebank Protein ID:	8895754
Ecogene ID:	EG12769
Ecocyc:	EG12769
ColiBase:	b3138
Kegg Gene:	b3138
EchoBASE ID:	EB2622
CCDB:	PTPB1_ECOLI
BacMap:	16131030

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Brinkkotter, A., Kloss, H., Alpert, C., Lengeler, J. W. (2000). "Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli." Mol Microbiol 37:125-135. Pubmed: 10931310
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Reizer, J., Ramseier, T. M., Reizer, A., Charbit, A., Saier, M. H. Jr (1996). "Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Microbiology 142 ( Pt 2):231-250. Pubmed: 8932697