Phosphoglycerol transferase I (P39401)

Identification
Name:Phosphoglycerol transferase I
Synonyms:
  • Phosphatidylglycerol--membrane-oligosaccharide glycerophosphotransferase
Gene Name:mdoB
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Transfers a phosphoglycerol residue from phosphatidylglycerol to the membrane-bound nascent glucan backbones
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Phosphatidylglycerol+1.0Diglucosyl-diacylglycerol ↔ 1.0Diacylglycerol+1.0Glycerophosphoglycoglycerolipid
1.0Phosphatidylglycerol + 1.0Diglucosyl-diacylglycerol ↔ 1.0Diacylglycerol + 1.0Glycerophosphoglycoglycerolipid
ReactionCard
1.0Thumb+1.0Membrane-derived-oligosaccharide D-glucose ↔ 1.01,2-Diacyl-sn-glycerol+1.0Membrane-derived-oligosaccharide 6-(glycerophospho)-D-glucose
1.0PG(16:0/16:0) + 1.0Membrane-derived-oligosaccharide D-glucose ↔ 1.01,2-Diacyl-sn-glycerol + 1.0Membrane-derived-oligosaccharide 6-(glycerophospho)-D-glucose
ReactionCard
EcoCyc Reactions:
1.0an MDO-O-glucose+1.0an L-1-phosphatidyl-glycerol ↔ 1.0a 1,2-diacylglycerol+1.0an MDO-6-(glycerophospho)-D-glucose
1.0an MDO-O-glucose + 1.0an L-1-phosphatidyl-glycerol ↔ 1.0a 1,2-diacylglycerol + 1.0an MDO-6-(glycerophospho)-D-glucose
ReactionCard
Complex Reactions:
1.0Phosphatidylglycerol+1.0membrane-derived-oligosaccharide D-glucose → 1.01,2-diacyl-sn-glycerol+1.0membrane-derived-oligosaccharide 6-(glycerophospho)-D-glucose
1.0Phosphatidylglycerol + 1.0membrane-derived-oligosaccharide D-glucose → 1.01,2-diacyl-sn-glycerol + 1.0membrane-derived-oligosaccharide 6-(glycerophospho)-D-glucose
ReactionCard
Metabolites:
ECMDB IDNameView
GO Classification:
Component
cell part
membrane
plasma membrane
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
phosphatidylglycerol-membrane-oligosaccharide glycerophosphotransferase activity
phosphotransferase activity, for other substituted phosphate groups
sulfuric ester hydrolase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
carbohydrate metabolic process
cellular glucan metabolic process
glucan biosynthetic process
glucan metabolic process
metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b4359
Gene OrientationCounterclockwise
Centisome Percentage:99.04
Left Sequence End4595173
Right Sequence End4597464
Gene Sequence:
>2292 bp
TTGTCAGAACTACTCTCTTTCGCCCTTTTTCTCGCCTCTGTGCTGATTTACGCATGGAAA
GCGGGACGTAACACCTGGTGGTTTGCAGCCACGTTAACGGTGCTGGGGCTATTTGTCGTT
TTAAATATCACCCTGTTTGCCAGCGACTATTTTACTGGCGATGGTATTAACGACGCGGTT
CTCTATACCTTAACCAACAGCCTGACCGGTGCTGGCGTCAGCAAATACATTTTGCCGGGT
ATCGGCATTGTGCTGGGGCTGACAGCGGTGTTCGGTGCGCTGGGCTGGATCCTGCGCCGT
CGTCGCCATCATCCGCACCATTTTGGTTACAGCCTGCTGGCGCTCTTACTGGCGCTGGGT
TCGGTGGACGCCAGCCCGGCATTTCGTCAGATAACGGAACTGGTGAAATCCCAGTCACGC
GACGGCGACCCGGACTTTGCGGCTTATTATAAAGAGCCGTCGAAAACTATCCCTGACCCG
AAACTCAACCTGGTTTATATCTACGGCGAAAGTCTCGAGCGGACCTATTTTGATAACGAG
GCTTTCCCGGATCTCACGCCTGAACTGGGCGCGTTGAAAAATGAAGGCCTGGATTTCAGC
CACACGCAGCAGCTGCCGGGAACAGATTACACGATTGCGGGCATGGTGGCTTCTCAGTGC
GGCATACCGCTGTTTGCCCCCTTTGAAGGCAACGCCTCCGCCTCTGTCTCCAGCTTCTTC
CCGCAGAACATCTGTCTGGGAGATATCCTGAAAAACTCGGGTTATCAGAACTATTTCGTG
CAGGGCGCGAATCTGCGTTTTGCCGGTAAAGATGTGTTCCTGAAGTCGCATGGCTTCGAC
CACTTATACGGCTCAGAAGAGCTGAAAAGCGTGGTAGCCGACCCGCACTATCGCAACGAC
TGGGGATTCTACGACGATACCGTTCTCGATGAAGCGTGGAAAAAGTTTGAAGAGCTTTCC
CGCTCAGGTCAGCGATTCTCACTGTTTACCCTGACAGTCGATACCCATCACCCGGATGGT
TTTATCTCTCGTACCTGTAACCGCAAAAAATATGATTTTGACGGTAAACCGAATCAGTCA
TTCAGCGCGGTAAGTTGCAGCCAGGAGAACATCGCGACGTTTATCAACAAAATCAAAGCG
TCACCGTGGTTTAAAGATACCGTCATCGTCGTCTCTTCTGACCATTTAGCGATGAACAAC
ACGGCGTGGAAATACCTCAATAAGCAGGATCGCAATAACCTGTTTTTTGTCATTCGTGGC
GACAAGCCGCAGCAAGAGACGCTGGCAGTGAAGCGTAACACGATGGATAACGGCGCGACG
GTGCTGGACATTCTCGGTGGCGATAACTATCTCGGACTTGGTCGTAGCAGTTTATCCGGG
CAGTCGATGTCGGAAATCTTCCTCAATATCAAAGAGAAAACATTGGCGTGGAAGCCAGAT
ATCATCCGCCTGTGGAAATTCCCTAAAGAGATGAAAGAGTTCACCATCGACCAGCAGAAA
AACATGATTGCCTTCTCGGGTAGCCATTTCCGTTTGCCGCTGCTGTTGCGGGTTTCAGAC
AAACGCGTGGAACCGCTGCCGGAAAGCGAATACTCAGCACCGCTGCGTTTCCAGCTGGCC
GATTTCGCTCCACGCGACAATTTCGTCTGGGTTGACCGTTGCTACAAGATGGCACAACTC
TGGGCTCCGGAACTGGCACTTTCCACCGACTGGTGTGTCTCGCAAGGGCAGCTTGGCGGT
CAGCAAATTGTTCAGCATGTTGACAAAACAACATGGCAGGGCAAAACGGCATTTAAAGAT
ACGGTCATCGACATGGCGCGTTACAAAGGCAATGTCGATACGCTGAAGATTGTTGATAAC
GATATTCGCTACAAAGCCGACAGTTTCATCTTTAACGTCGCCGGTGCGCCAGAAGAGGTG
AAACAGTTTAGCGGGATTTCCCGTCCGGAGTCGTGGGGCCGCTGGTCCAACGCGCAGCTG
GGCGATGAAGTAAAAATCGAGTACAAGCATCCGCTGCCGAAGAAATTTGACCTGGTGATT
ACCGCCAAAGCATACGGCAATAACGCCAGCCGTCCTATTCCGGTACGCGTAGGCAATGAA
GAACAAACTCTTGTGCTGGGCAATGAAGTGACCACCACCACACTGCATTTCGATAACCCA
ACCGATGCCGACACACTGGTAATTGTGCCGCCGGAACCTGTCTCAACCAACGAAGGGAAT
ATCCTCGGACACTCGCCGCGTAAGCTCGGGATCGGCATGGTGGAAATTAAAGTGGTAGAA
CGTGAAGGGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:763
Protein Molecular Weight:85493
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • 1-21
  • 26-46
  • 77-97
  • 108-128
Protein Sequence:
>Phosphoglycerol transferase I
MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNITLFASDYFTGDGINDAV
LYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRRHHPHHFGYSLLALLLALG
SVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDPKLNLVYIYGESLERTYFDNE
AFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVASQCGIPLFAPFEGNASASVSSFF
PQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKSHGFDHLYGSEELKSVVADPHYRND
WGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVDTHHPDGFISRTCNRKKYDFDGKPNQS
FSAVSCSQENIATFINKIKASPWFKDTVIVVSSDHLAMNNTAWKYLNKQDRNNLFFVIRG
DKPQQETLAVKRNTMDNGATVLDILGGDNYLGLGRSSLSGQSMSEIFLNIKEKTLAWKPD
IIRLWKFPKEMKEFTIDQQKNMIAFSGSHFRLPLLLRVSDKRVEPLPESEYSAPLRFQLA
DFAPRDNFVWVDRCYKMAQLWAPELALSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKD
TVIDMARYKGNVDTLKIVDNDIRYKADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQL
GDEVKIEYKHPLPKKFDLVITAKAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNP
TDADTLVIVPPEPVSTNEGNILGHSPRKLGIGMVEIKVVEREG
References
External Links:
ResourceLink
Uniprot ID:P39401
Uniprot Name:OPGB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85677099
Ecogene ID:EG12591
Ecocyc:EG12591
ColiBase:b4359
Kegg Gene:b4359
EchoBASE ID:EB2476
CCDB:OPGB_ECOLI
BacMap:90111741
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Fiedler, W., Rotering, H. (1985). "Characterization of an Escherichia coli mdoB mutant strain unable to transfer sn-1-phosphoglycerol to membrane-derived oligosaccharides." J Biol Chem 260:4799-4806. Pubmed: 2985566
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jackson, B. J., Bohin, J. P., Kennedy, E. P. (1984). "Biosynthesis of membrane-derived oligosaccharides: characterization of mdoB mutants defective in phosphoglycerol transferase I activity." J Bacteriol 160:976-981. Pubmed: 6094515