| Identification |
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| Name: | Phosphoglycerol transferase I |
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| Synonyms: | - Phosphatidylglycerol--membrane-oligosaccharide glycerophosphotransferase
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| Gene Name: | mdoB |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | Involved in catalytic activity |
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| Specific Function: | Transfers a phosphoglycerol residue from phosphatidylglycerol to the membrane-bound nascent glucan backbones |
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| Cellular Location: | Cell inner membrane; Multi-pass membrane protein |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | |
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| KEGG Reactions: | |
1.0Phosphatidylglycerol | + | 1.0Diglucosyl-diacylglycerol | ↔ | 1.0Diacylglycerol | + | 1.0Glycerophosphoglycoglycerolipid |
| 1.0Phosphatidylglycerol + 1.0Diglucosyl-diacylglycerol ↔ 1.0Diacylglycerol + 1.0Glycerophosphoglycoglycerolipid ReactionCard | |
1.0 | + | 1.0Membrane-derived-oligosaccharide D-glucose | ↔ | 1.01,2-Diacyl-sn-glycerol | + | 1.0Membrane-derived-oligosaccharide 6-(glycerophospho)-D-glucose |
| 1.0 PG(16:0/16:0) + 1.0Membrane-derived-oligosaccharide D-glucose ↔ 1.01,2-Diacyl-sn-glycerol + 1.0Membrane-derived-oligosaccharide 6-(glycerophospho)-D-glucose ReactionCard |
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| EcoCyc Reactions: | |
1.0an MDO-O-glucose | + | 1.0an L-1-phosphatidyl-glycerol | ↔ | 1.0a 1,2-diacylglycerol | + | 1.0an MDO-6-(glycerophospho)-D-glucose |
| 1.0an MDO-O-glucose + 1.0an L-1-phosphatidyl-glycerol ↔ 1.0a 1,2-diacylglycerol + 1.0an MDO-6-(glycerophospho)-D-glucose ReactionCard |
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| Complex Reactions: | |
1.0Phosphatidylglycerol | + | 1.0membrane-derived-oligosaccharide D-glucose | → | 1.01,2-diacyl-sn-glycerol | + | 1.0membrane-derived-oligosaccharide 6-(glycerophospho)-D-glucose |
| 1.0Phosphatidylglycerol + 1.0membrane-derived-oligosaccharide D-glucose → 1.01,2-diacyl-sn-glycerol + 1.0membrane-derived-oligosaccharide 6-(glycerophospho)-D-glucose ReactionCard |
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| Metabolites: | |
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| GO Classification: | | Component |
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| cell part | | membrane | | plasma membrane | | Function |
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| catalytic activity | | hydrolase activity | | hydrolase activity, acting on ester bonds | | phosphatidylglycerol-membrane-oligosaccharide glycerophosphotransferase activity | | phosphotransferase activity, for other substituted phosphate groups | | sulfuric ester hydrolase activity | | transferase activity | | transferase activity, transferring phosphorus-containing groups | | Process |
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| carbohydrate metabolic process | | cellular glucan metabolic process | | glucan biosynthetic process | | glucan metabolic process | | metabolic process | | polysaccharide metabolic process | | primary metabolic process |
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| Gene Properties |
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| Blattner: | b4359 |
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| Gene Orientation | Counterclockwise |
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| Centisome Percentage: | 99.04 |
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| Left Sequence End | 4595173 |
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| Right Sequence End | 4597464 |
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| Gene Sequence: | >2292 bp
TTGTCAGAACTACTCTCTTTCGCCCTTTTTCTCGCCTCTGTGCTGATTTACGCATGGAAA
GCGGGACGTAACACCTGGTGGTTTGCAGCCACGTTAACGGTGCTGGGGCTATTTGTCGTT
TTAAATATCACCCTGTTTGCCAGCGACTATTTTACTGGCGATGGTATTAACGACGCGGTT
CTCTATACCTTAACCAACAGCCTGACCGGTGCTGGCGTCAGCAAATACATTTTGCCGGGT
ATCGGCATTGTGCTGGGGCTGACAGCGGTGTTCGGTGCGCTGGGCTGGATCCTGCGCCGT
CGTCGCCATCATCCGCACCATTTTGGTTACAGCCTGCTGGCGCTCTTACTGGCGCTGGGT
TCGGTGGACGCCAGCCCGGCATTTCGTCAGATAACGGAACTGGTGAAATCCCAGTCACGC
GACGGCGACCCGGACTTTGCGGCTTATTATAAAGAGCCGTCGAAAACTATCCCTGACCCG
AAACTCAACCTGGTTTATATCTACGGCGAAAGTCTCGAGCGGACCTATTTTGATAACGAG
GCTTTCCCGGATCTCACGCCTGAACTGGGCGCGTTGAAAAATGAAGGCCTGGATTTCAGC
CACACGCAGCAGCTGCCGGGAACAGATTACACGATTGCGGGCATGGTGGCTTCTCAGTGC
GGCATACCGCTGTTTGCCCCCTTTGAAGGCAACGCCTCCGCCTCTGTCTCCAGCTTCTTC
CCGCAGAACATCTGTCTGGGAGATATCCTGAAAAACTCGGGTTATCAGAACTATTTCGTG
CAGGGCGCGAATCTGCGTTTTGCCGGTAAAGATGTGTTCCTGAAGTCGCATGGCTTCGAC
CACTTATACGGCTCAGAAGAGCTGAAAAGCGTGGTAGCCGACCCGCACTATCGCAACGAC
TGGGGATTCTACGACGATACCGTTCTCGATGAAGCGTGGAAAAAGTTTGAAGAGCTTTCC
CGCTCAGGTCAGCGATTCTCACTGTTTACCCTGACAGTCGATACCCATCACCCGGATGGT
TTTATCTCTCGTACCTGTAACCGCAAAAAATATGATTTTGACGGTAAACCGAATCAGTCA
TTCAGCGCGGTAAGTTGCAGCCAGGAGAACATCGCGACGTTTATCAACAAAATCAAAGCG
TCACCGTGGTTTAAAGATACCGTCATCGTCGTCTCTTCTGACCATTTAGCGATGAACAAC
ACGGCGTGGAAATACCTCAATAAGCAGGATCGCAATAACCTGTTTTTTGTCATTCGTGGC
GACAAGCCGCAGCAAGAGACGCTGGCAGTGAAGCGTAACACGATGGATAACGGCGCGACG
GTGCTGGACATTCTCGGTGGCGATAACTATCTCGGACTTGGTCGTAGCAGTTTATCCGGG
CAGTCGATGTCGGAAATCTTCCTCAATATCAAAGAGAAAACATTGGCGTGGAAGCCAGAT
ATCATCCGCCTGTGGAAATTCCCTAAAGAGATGAAAGAGTTCACCATCGACCAGCAGAAA
AACATGATTGCCTTCTCGGGTAGCCATTTCCGTTTGCCGCTGCTGTTGCGGGTTTCAGAC
AAACGCGTGGAACCGCTGCCGGAAAGCGAATACTCAGCACCGCTGCGTTTCCAGCTGGCC
GATTTCGCTCCACGCGACAATTTCGTCTGGGTTGACCGTTGCTACAAGATGGCACAACTC
TGGGCTCCGGAACTGGCACTTTCCACCGACTGGTGTGTCTCGCAAGGGCAGCTTGGCGGT
CAGCAAATTGTTCAGCATGTTGACAAAACAACATGGCAGGGCAAAACGGCATTTAAAGAT
ACGGTCATCGACATGGCGCGTTACAAAGGCAATGTCGATACGCTGAAGATTGTTGATAAC
GATATTCGCTACAAAGCCGACAGTTTCATCTTTAACGTCGCCGGTGCGCCAGAAGAGGTG
AAACAGTTTAGCGGGATTTCCCGTCCGGAGTCGTGGGGCCGCTGGTCCAACGCGCAGCTG
GGCGATGAAGTAAAAATCGAGTACAAGCATCCGCTGCCGAAGAAATTTGACCTGGTGATT
ACCGCCAAAGCATACGGCAATAACGCCAGCCGTCCTATTCCGGTACGCGTAGGCAATGAA
GAACAAACTCTTGTGCTGGGCAATGAAGTGACCACCACCACACTGCATTTCGATAACCCA
ACCGATGCCGACACACTGGTAATTGTGCCGCCGGAACCTGTCTCAACCAACGAAGGGAAT
ATCCTCGGACACTCGCCGCGTAAGCTCGGGATCGGCATGGTGGAAATTAAAGTGGTAGAA
CGTGAAGGGTAA |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 763 |
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| Protein Molecular Weight: | 85493 |
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| Protein Theoretical pI: | 7 |
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| Signaling Regions: | |
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| Transmembrane Regions: | |
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| Protein Sequence: | >Phosphoglycerol transferase I
MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNITLFASDYFTGDGINDAV
LYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRRHHPHHFGYSLLALLLALG
SVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDPKLNLVYIYGESLERTYFDNE
AFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVASQCGIPLFAPFEGNASASVSSFF
PQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKSHGFDHLYGSEELKSVVADPHYRND
WGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVDTHHPDGFISRTCNRKKYDFDGKPNQS
FSAVSCSQENIATFINKIKASPWFKDTVIVVSSDHLAMNNTAWKYLNKQDRNNLFFVIRG
DKPQQETLAVKRNTMDNGATVLDILGGDNYLGLGRSSLSGQSMSEIFLNIKEKTLAWKPD
IIRLWKFPKEMKEFTIDQQKNMIAFSGSHFRLPLLLRVSDKRVEPLPESEYSAPLRFQLA
DFAPRDNFVWVDRCYKMAQLWAPELALSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKD
TVIDMARYKGNVDTLKIVDNDIRYKADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQL
GDEVKIEYKHPLPKKFDLVITAKAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNP
TDADTLVIVPPEPVSTNEGNILGHSPRKLGIGMVEIKVVEREG |
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| References |
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| External Links: | |
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| General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
- Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
- Fiedler, W., Rotering, H. (1985). "Characterization of an Escherichia coli mdoB mutant strain unable to transfer sn-1-phosphoglycerol to membrane-derived oligosaccharides." J Biol Chem 260:4799-4806. Pubmed: 2985566
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Jackson, B. J., Bohin, J. P., Kennedy, E. P. (1984). "Biosynthesis of membrane-derived oligosaccharides: characterization of mdoB mutants defective in phosphoglycerol transferase I activity." J Bacteriol 160:976-981. Pubmed: 6094515
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