N-acetylneuraminate epimerase (P39371)

Identification
Name:N-acetylneuraminate epimerase
Synonyms:
  • N-acetylneuraminate mutarotase
  • Neu5Ac mutarotase
  • Sialic acid epimerase
Gene Name:nanM
Enzyme Class:
Biological Properties
General Function:Involved in racemase and epimerase activity, acting on
Specific Function:Converts alpha-N-acetylneuranimic acid (Neu5Ac) to the beta-anomer, accelerating the equilibrium between the alpha- and beta-anomers. Probably facilitates sialidase-negative bacteria to compete sucessfully for limited amounts of extracellular Neu5Ac, which is likely taken up in the beta-anomer. In addition, the rapid removal of sialic acid from solution might be advantageous to the bacterium to damp down host responses
Cellular Location:Periplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb
1.0alpha-N-acetylneuraminate ↔ 1.0N-Acetylneuraminic acid
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb
1.0N-Acetyl-alpha-neuraminate → 1.0N-Acetylneuraminic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB23217N-Acetyl-alpha-neuraminateMetaboCard
ECMDB00830N-Acetylneuraminic acidMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1107 bp
ATGAATAAAACAATAACGGCGCTTGCTATCATGATGGCTTCATTTGCCGCAAACGCGTCT
GTATTACCGGAAACTCCTGTGCCATTTAAAAGTGGTACCGGAGCAATTGATAACGACACT
GTCTACATTGGTTTAGGTAGCGCAGGTACGGCATGGTACAAGCTGGATACACAGGCCAAA
GATAAAAAATGGACAGCGTTAGCTGCATTCCCTGGCGGACCAAGAGATCAAGCAACCTCT
GCATTTATTGATGGCAATCTGTATGTGTTTGGCGGCATTGGCAAAAACAGCGAGGGCTTG
ACTCAGGTATTTAATGACGTACACAAATACAACCCCAAAACCAATAGTTGGGTTAAATTG
ATGTCGCACGCGCCGATGGGCATGGCGGGCCATGTGACTTTTGTACACAACGGCAAGGCT
TATGTTACTGGTGGTGTTAACCAGAATATCTTCAATGGCTATTTTGAAGATCTCAACGAG
GCTGGAAAAGATTCAACCGCTATAGATAAAATCAATGCTCACTATTTTGACAAAAAAGCA
GAAGATTATTTCTTCAATAAGTTTCTGTTGTCTTTTGATCCCTCAACACAGCAATGGAGT
TACGCTGGCGAATCGCCCTGGTACGGAACGGCTGGTGCGGCGGTTGTGAATAAAGGTGAT
AAAACCTGGCTTATTAATGGCGAAGCCAAACCAGGATTGCGAACGGATGCCGTATTTGAA
CTTGATTTCACCGGTAATAATTTAAAATGGAATAAGCTTGCTCCCGTCTCATCACCAGAT
GGCGTAGCTGGCGGTTTTGCGGGGATAAGCAATGATTCTCTTATATTTGCCGGAGGGGCC
GGATTCAAAGGTTCACGAGAAAATTACCAGAACGGTAAGAACTATGCGCATGAAGGCCTG
AAAAAATCATATAGCACTGATATTCATCTTTGGCATAACGGGAAATGGGATAAATCGGGT
GAATTATCGCAAGGTCGGGCCTACGGAGTATCATTGCCCTGGAATAATAGTCTATTGATT
ATTGGCGGTGAAACTGCAGGCGGCAAAGCGGTGACGGATTCAGTTTTGATCACTGTGAAG
GATAATAAAGTCACAGTACAAAACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:368
Protein Molecular Weight:39572
Protein Theoretical pI:8
Signaling Regions:
  • 1-19
Transmembrane Regions:
  • None
Protein Sequence:
>N-acetylneuraminate epimerase
MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIGLGSAGTAWYKLDTQAK
DKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQVFNDVHKYNPKTNSWVKL
MSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNEAGKDSTAIDKINAHYFDKKA
EDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNKGDKTWLINGEAKPGLRTDAVFE
LDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIFAGGAGFKGSRENYQNGKNYAHEGL
KKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPWNNSLLIIGGETAGGKAVTDSVLITVK
DNKVTVQN
References
External Links:
ResourceLink
Uniprot ID:P39371
Uniprot Name:NANM_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:87082419
CCDB:NANM_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Condemine, G., Berrier, C., Plumbridge, J., Ghazi, A. (2005). "Function and expression of an N-acetylneuraminic acid-inducible outer membrane channel in Escherichia coli." J Bacteriol 187:1959-1965. Pubmed: 15743943
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Severi, E., Muller, A., Potts, J. R., Leech, A., Williamson, D., Wilson, K. S., Thomas, G. H. (2008). "Sialic acid mutarotation is catalyzed by the Escherichia coli beta-propeller protein YjhT." J Biol Chem 283:4841-4849. Pubmed: 18063573