dTDP-glucose 4,6-dehydratase 1 (P37759)

Identification
Name:dTDP-glucose 4,6-dehydratase 1
Synonyms:Not Available
Gene Name:rfbB
Enzyme Class:
Biological Properties
General Function:Involved in dTDP-glucose 4,6-dehydratase activity
Specific Function:Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0dTDP-D-Glucose ↔ 1.04,6-Dideoxy-4-oxo-dTDP-D-glucose + 1.0Water
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0dTDP-D-Glucose ↔ 1.04,6-Dideoxy-4-oxo-dTDP-D-glucose + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB013994,6-Dideoxy-4-oxo-dTDP-D-glucoseMetaboCard
ECMDB01328dTDP-D-GlucoseMetaboCard
ECMDB20298TDP-GlucoseMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
binding
carbon-oxygen lyase activity
catalytic activity
coenzyme binding
cofactor binding
dTDP-glucose 4,6-dehydratase activity
hydro-lyase activity
lyase activity
Process
cellular metabolic process
cellular nitrogen compound metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleotide-sugar metabolic process
Gene Properties
Blattner:b2041
Gene OrientationCounterclockwise
Centisome Percentage:45.48
Left Sequence End2110000
Right Sequence End2111085
Gene Sequence:
>1086 bp
ATGGAAAGTGGTCATCGCTTTGATGCTCAGACGCTGCACAGTTTTATTCAGGCTGTATTT
CGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCA
AACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGG
AGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCG
GTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTG
GGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCAT
ATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCAC
TTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTT
GGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGAT
TACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCC
GTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATG
CAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCA
GCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACG
TTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAA
CTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGAAAGCC
TCGCCGCATGATGATGATAAGCCGATTTTGCTACCGGGCGAGTGGGAAGTGAACACGCGT
CGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGAT
GCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCC
AGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:361
Protein Molecular Weight:40558
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>dTDP-glucose 4,6-dehydratase 1
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNRESLADVSDSERYVFEHADIC
DAPAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDS
DKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTAYAPSSPYSASKASSDHLVRA
WKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVADRPGH
DRRYAIDAEKIGRALGWKPQETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEQNYEGR
Q
References
External Links:
ResourceLink
Uniprot ID:P37759
Uniprot Name:RMLB1_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062362
Ecogene ID:EG12412
Ecocyc:EG12412
ColiBase:b2041
Kegg Gene:b2041
EchoBASE ID:EB2311
CCDB:RMLB1_ECOLI
BacMap:16129981
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Stevenson, G., Neal, B., Liu, D., Hobbs, M., Packer, N. H., Batley, M., Redmond, J. W., Lindquist, L., Reeves, P. (1994). "Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster." J Bacteriol 176:4144-4156. Pubmed: 7517391