2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (P37355)

Identification
Name:2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
Synonyms:
  • SHCHC synthase
Gene Name:menH
Enzyme Class:
Biological Properties
General Function:Involved in 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase activity
Specific Function:Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also able to catalyze the hydrolysis of the thioester bond in palmitoyl-CoA in vitro
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Metabolic pathways eco01100
  • Ubiquinone and other terpenoid-quinone biosynthesis ec00130
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate ↔ 1.0(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + 1.0Pyruvic acid
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.02-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate → 1.0(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + 1.0Pyruvic acid
ReactionCard
SMPDB Reactions:
1.0Thumb → 1.0(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate+1.0Thumb+1.0Thumb
1.02-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate → 1.0(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + 1.0Pyruvic acid + 1.0(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate ↔ 1.0(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + 1.0Pyruvic acid
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.02-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate → 1.0(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + 1.0Pyruvic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB20002(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylateMetaboCard
ECMDB200502-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylateMetaboCard
ECMDB00243Pyruvic acidMetaboCard
GO Classification:
Function
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase activity
carbon-oxygen lyase activity
catalytic activity
lyase activity
Process
fat-soluble vitamin metabolic process
menaquinone biosynthetic process
menaquinone metabolic process
metabolic process
small molecule metabolic process
vitamin K metabolic process
vitamin metabolic process
Gene Properties
Blattner:b2263
Gene OrientationCounterclockwise
Centisome Percentage:51.19
Left Sequence End2374856
Right Sequence End2375614
Gene Sequence:
>759 bp
ATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCG
GTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGT
GGCCCAGGTAACAGCACCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGC
ATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTC
GTGGAAGCAGACATCCGCAACTGGCAACCGGTACAGGCACTCGATCTGATTTTTGCTAAT
GCCTCACTGCAATGGCTGCCCGACCACTACGAATTGTTTCCTCATCTGGTTTCTTTACTT
AATCCGCAGGGCGTGCTGGCAGTACAGATGCCAGATAACTGGCTGGAGCCGACCCATGTG
CTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCT
GGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGA
ACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACT
GGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGC
TACCATCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCA
TTTCCGCGTCTGTTTATTGTTGCCCGGCGTATGGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:252
Protein Molecular Weight:27682
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLPGHGGSAAISVDG
FDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAGLCGVIVEGGHPGLQNAEQ
RAERQRSDRQWVQRFLTEPLTAVFADWYQQPVFASLNDDQRRELVALRSNNNGATLAAML
EATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPA
GVIASLAQILRF
References
External Links:
ResourceLink
Uniprot ID:P37355
Uniprot Name:MENH_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675003
Ecogene ID:EG12438
Ecocyc:EG12438
ColiBase:b2263
Kegg Gene:b2263
EchoBASE ID:EB2333
CCDB:MENH_ECOLI
BacMap:16130198
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jiang, M., Chen, X., Guo, Z. F., Cao, Y., Chen, M., Guo, Z. (2008). "Identification and characterization of (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase in the menaquinone biosynthesis of Escherichia coli." Biochemistry 47:3426-3434. Pubmed: 18284213
  • Kuznetsova, E., Proudfoot, M., Sanders, S. A., Reinking, J., Savchenko, A., Arrowsmith, C. H., Edwards, A. M., Yakunin, A. F. (2005). "Enzyme genomics: Application of general enzymatic screens to discover new enzymes." FEMS Microbiol Rev 29:263-279. Pubmed: 15808744
  • Sharma, V., Suvarna, K., Meganathan, R., Hudspeth, M. E. (1992). "Menaquinone (vitamin K2) biosynthesis: nucleotide sequence and expression of the menB gene from Escherichia coli." J Bacteriol 174:5057-5062. Pubmed: 1629162
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837