ABC transporter ATP-binding protein yojI (P33941)

Identification
Name:ABC transporter ATP-binding protein yojI
Synonyms:Not Available
Gene Name:yojI
Enzyme Class:Not Available
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Mediates resistance to the antibacterial peptide microcin J25. Functions as an efflux pump for microcin J25. Requires tolC for export of microcin J25
Cellular Location:Cell inner membrane; Multi-pass membrane protein (Probable)
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Function
active transmembrane transporter activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ATPase activity
ATPase activity, coupled to transmembrane movement of substances
binding
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
P-P-bond-hydrolysis-driven transmembrane transporter activity
peptide transporter activity
primary active transmembrane transporter activity
purine nucleoside binding
pyrophosphatase activity
substrate-specific transporter activity
transmembrane transporter activity
transporter activity
Process
establishment of localization
peptide transport
transmembrane transport
transport
Gene Properties
Blattner:b2211
Gene OrientationCounterclockwise
Centisome Percentage:49.68
Left Sequence End2304994
Right Sequence End2306637
Gene Sequence:
>1644 bp
ATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAAT
ACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATT
ATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGCTGGGTTGGCAATGGTGTCTACGTT
TCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACG
CAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCC
ACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTG
GTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTG
GTGAATCAGTTTAAAACCACCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATC
GCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAAC
GATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGAT
CAACGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAG
GATGGCGGCACTTATAACATCACGATGGCCAATGGTTACTCACTGGTTCAGGGAAGTACG
GCGCGGCAACTGGCGGCAGTTCCTTCCAGCGCTGACCCTTCTCGTACGACTGTCGCTTAT
GTTGATGGGACGGCAGGCAATATTGAGATCCCGGAGAAATTACTGAATACCGGGTCGCTG
GGCGGCATTCTGACATTCCGTTCTCAGGATCTGGACCAGACGCGTAATACGCTTGGACAA
CTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAAC
GGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAA
AACAAAGGTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACA
GATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACC
ACTTTTACGGTGACGCCGGATGCCAACGGTAAAGTGGCATTTGATGGTCTGGAGTTGACG
TTTACAGGAACGCCTGCCGTTAACGACAGCTTCACGCTGAAACCAGTAAGTGACGCCATC
GTCAACATGGATGTATTAATCACCGACGAAGCGAAAATAGCGATGGCGAGCGAAGAAGAT
GCGGGTGATAGCGATAACCGCAACGGTCAGGCCCTGCTGGATCTGCAAAGCAACAGTAAA
ACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGT
AATAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTT
TCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAA
CGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTT
GATGCGCTGATTAACATTCGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:547
Protein Molecular Weight:61552
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • 16-36
  • 48-68
  • 125-145
  • 149-169
  • 240-260
  • 270-290
Protein Sequence:
>ABC transporter ATP-binding protein yojI
MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETADTSLLVLPEFLGLLLL
LMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQLGSASLLAGLTSDVRNITI
AFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAITIWGGFVLVARVYKHMATLRE
TEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDAQEYRHHIIRADTFHLSAVNWSN
IMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLRTPLLSAVGALPTLLTAQVAFNKLN
KFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQDNAFSVGPINLTIKRGELLFLIGGNGS
GKSTLAMLLTGLYQPQSGEILLDGKPVSGEQPEDYRKLFSAVFTDVWLFDQLLGPEGKPA
NPQLVEKWLAQLKMAHKLELSNGRIVNLKLSKGQKKRVALLLALAEERDIILLDEWAADQ
DPHFRREFYQVLLPLMQEMGKTIFAISHDDHYFIHADRLLEMRNGQLSELTGEERDAASR
DAVARTA
References
External Links:
ResourceLink
Uniprot ID:P33941
Uniprot Name:YOJI_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651528
Ecogene ID:EG12070
Ecocyc:EG12070
ColiBase:b2211
Kegg Gene:b2211
EchoBASE ID:EB1997
CCDB:YOJI_ECOLI
BacMap:16130148
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Delgado, M. A., Vincent, P. A., Farias, R. N., Salomon, R. A. (2005). "YojI of Escherichia coli functions as a microcin J25 efflux pump." J Bacteriol 187:3465-3470. Pubmed: 15866933
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040