Ribosomal large subunit pseudouridine synthase D (P33643)

Identification
Name:Ribosomal large subunit pseudouridine synthase D
Synonyms:
  • rRNA pseudouridylate synthase D
  • rRNA-uridine isomerase D
Gene Name:rluD
Enzyme Class:
Biological Properties
General Function:Translation, ribosomal structure and biogenesis
Specific Function:Responsible for synthesis of pseudouridine from uracil at positions 1911, 1915 and 1917 in 23S ribosomal RNA
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
1.023S rRNA uridine(1911)/uridine(1915)/uridine(1917) → 1.023S rRNA pseudouridine(1911)/pseudouridine(1915)/pseudouridine(1917)
1.023S rRNA uridine(1911)/uridine(1915)/uridine(1917) → 1.023S rRNA pseudouridine(1911)/pseudouridine(1915)/pseudouridine(1917)
ReactionCard
Metabolites:
ECMDB IDNameView
GO Classification:
Function
binding
catalytic activity
intramolecular transferase activity
isomerase activity
nucleic acid binding
pseudouridine synthase activity
RNA binding
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
pseudouridine synthesis
RNA metabolic process
RNA modification
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>981 bp
ATGGCACAACGAGTACAGCTCACTGCAACGGTGTCCGAAAACCAACTCGGTCAACGCTTA
GATCAGGCTTTGGCCGAAATGTTCCCGGATTATTCACGTTCGCGAATAAAAGAATGGATC
CTCGACCAGCGAGTGCTGGTTAACGGCAAAGTTTGTGATAAGCCGAAAGAAAAAGTATTG
GGTGGCGAGCAGGTTGCCATCAACGCTGAGATTGAAGAAGAAGCGCGTTTTGAACCGCAG
GATATCCCGCTGGATATCGTCTATGAAGATGAAGACATTATTATCATTAATAAACCGCGC
GACCTGGTGGTACATCCTGGCGCGGGTAACCCGGATGGCACGGTACTGAATGCGTTGCTT
CATTACTATCCACCCATTGCCGATGTACCGCGTGCGGGCATCGTCCATCGTCTGGATAAA
GACACCACTGGCCTGATGGTTGTGGCAAAAACCGTTCCGGCTCAGACGCGTTTAGTCGAA
TCTTTGCAACGGCGTGAAATTACTCGTGAGTATGAAGCGGTGGCGATTGGTCATATGACC
GCAGGTGGCACGGTGGACGAGCCAATCAGTCGCCACCCGACCAAACGTACCCATATGGCG
GTGCATCCGATGGGCAAACCAGCGGTGACTCACTATCGCATCATGGAACACTTCCGTGTG
CACACGCGTCTGCGGTTGCGTCTGGAAACTGGACGTACGCACCAGATCCGCGTGCATATG
GCCCATATCACTCATCCGCTGGTGGGCGATCCGGTTTATGGTGGCCGTCCGCGTCCGCCA
AAAGGTGCTTCGGAAGCATTTATCTCCACGCTGCGTAAGTTTGACCGCCAGGCGCTACAT
GCAACCATGCTGCGTCTTTATCACCCGATCTCCGGCATCGAAATGGAATGGCATGCGCCT
ATTCCACAAGATATGGTGGAGCTGATTGAGGTGATGCGCGCCGATTTCGAAGAACATAAG
GATGAAGTGGACTGGTTATGA
Protein Properties
Pfam Domain Function:
Protein Residues:326
Protein Molecular Weight:37121
Protein Theoretical pI:7
PDB File:1V9F
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribosomal large subunit pseudouridine synthase D
MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQRVLVNGKVCDKPKEKVL
GGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIIINKPRDLVVHPGAGNPDGTVLNALL
HYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVESLQRREITREYEAVAIGHMT
AGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHFRVHTRLRLRLETGRTHQIRVHM
AHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDRQALHATMLRLYHPISGIEMEWHAP
IPQDMVELIEVMRADFEEHKDEVDWL
References
External Links:
ResourceLink
Uniprot ID:P33643
Uniprot Name:RLUD_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:1788946
PDB ID:1V9F
CCDB:RLUD_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gutgsell, N. S., Del Campo, M., Raychaudhuri, S., Ofengand, J. (2001). "A second function for pseudouridine synthases: A point mutant of RluD unable to form pseudouridines 1911, 1915, and 1917 in Escherichia coli 23S ribosomal RNA restores normal growth to an RluD-minus strain." RNA 7:990-998. Pubmed: 11453071
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kitagawa, M., Wada, C., Yoshioka, S., Yura, T. (1991). "Expression of ClpB, an analog of the ATP-dependent protease regulatory subunit in Escherichia coli, is controlled by a heat shock sigma factor (sigma 32)." J Bacteriol 173:4247-4253. Pubmed: 1906060
  • Raychaudhuri, S., Conrad, J., Hall, B. G., Ofengand, J. (1998). "A pseudouridine synthase required for the formation of two universally conserved pseudouridines in ribosomal RNA is essential for normal growth of Escherichia coli." RNA 4:1407-1417. Pubmed: 9814761
  • Wrzesinski, J., Bakin, A., Ofengand, J., Lane, B. G. (2000). "Isolation and properties of Escherichia coli 23S-RNA pseudouridine 1911, 1915, 1917 synthase (RluD)." IUBMB Life 50:33-37. Pubmed: 11087118
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837