D-alanyl-D-alanine carboxypeptidase dacD (P33013)

Identification
Name:D-alanyl-D-alanine carboxypeptidase dacD
Synonyms:
  • DD-carboxypeptidase
  • DD-peptidase
  • Penicillin-binding protein 6b
  • PBP-6b
Gene Name:dacD
Enzyme Class:
Biological Properties
General Function:Involved in carboxypeptidase activity
Specific Function:Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors
Cellular Location:Cell inner membrane; Peripheral membrane protein (Potential).
SMPDB Pathways:Not Available
KEGG Pathways:
EcoCyc Reactions:
1.0a lipid II+1.0Thumb ? 1.0a N-acetylglucosamine--N-acetylmuramyl-(tetrapeptide) pyrophosphoryl-undecaprenol+1.0Thumb
1.0a lipid II + 1.0Water ? 1.0a N-acetylglucosamine--N-acetylmuramyl-(tetrapeptide) pyrophosphoryl-undecaprenol + 1.0D-Alanine
ReactionCard
Complex Reactions:
1.0Thumb+1.0three disacharide linked murein units (pentapeptide crosslinked tetrapeptide (A2pm->D-ala) tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain) → 1.0Thumb+1.0three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala) & tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain)
1.0Water + 1.0three disacharide linked murein units (pentapeptide crosslinked tetrapeptide (A2pm->D-ala) tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain) → 1.0D-Alanine + 1.0three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala) & tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain)
ReactionCard
1.0Thumb+1.0two disacharide linked murein units, pentapeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain) → 1.0Thumb+1.0two disacharide linked murein units, tetrapeptide corsslinked tetrapeptide (A2pm->D-ala) (middle of chain)
1.0Water + 1.0two disacharide linked murein units, pentapeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain) → 1.0D-Alanine + 1.0two disacharide linked murein units, tetrapeptide corsslinked tetrapeptide (A2pm->D-ala) (middle of chain)
ReactionCard
1.0Thumb+1.0two linked disacharide pentapeptide and tetrapeptide murein units (uncrosslinked, middle of chain) → 1.0Thumb+1.0two linked disacharide tetrapeptide murein units (uncrosslinked, middle of chain)
1.0Water + 1.0two linked disacharide pentapeptide and tetrapeptide murein units (uncrosslinked, middle of chain) → 1.0D-Alanine + 1.0two linked disacharide tetrapeptide murein units (uncrosslinked, middle of chain)
ReactionCard
1.0Thumb+1.0two linked disacharide pentapeptide and tripeptide murein units (uncrosslinked, middle of chain) → 1.0Thumb+1.0two linked disacharide tetrapeptide and tripeptide murein units (uncrosslinked, middle of chain)
1.0Water + 1.0two linked disacharide pentapeptide and tripeptide murein units (uncrosslinked, middle of chain) → 1.0D-Alanine + 1.0two linked disacharide tetrapeptide and tripeptide murein units (uncrosslinked, middle of chain)
ReactionCard
1.0Thumb+1.0two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → 1.0Thumb+1.0two linked disacharide pentapeptide and tetrapeptide murein units (uncrosslinked, middle of chain)
1.0Water + 1.0two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → 1.0D-Alanine + 1.0two linked disacharide pentapeptide and tetrapeptide murein units (uncrosslinked, middle of chain)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01310D-AlanineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
carboxypeptidase activity
catalytic activity
exopeptidase activity
hydrolase activity
peptidase activity
peptidase activity, acting on L-amino acid peptides
serine-type carboxypeptidase activity
serine-type D-Ala-D-Ala carboxypeptidase activity
Process
macromolecule metabolic process
metabolic process
protein metabolic process
proteolysis
Gene Properties
Blattner:b2010
Gene OrientationCounterclockwise
Centisome Percentage:44.82
Left Sequence End2079405
Right Sequence End2080571
Gene Sequence:
>1167 bp
GTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATT
GCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGAC
TTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTG
TTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAA
CGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCC
GCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCG
ATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTA
CTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCT
ATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCG
ATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGT
GGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTG
AAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAA
GAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCG
TATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACG
CTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAA
CCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCT
GAGGGAACGACTTATCAGCAAATTATGGTGGTGGGGATCCTGTGCGGTATCGGTTTTACT
ATGTCTATCTTTATTGCCAGCCTGGCCTTTGGTAGCGTAGATCCAGAACTGATTAACTGG
GCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTA
CGCGTTCGTTTGCGTCCATCAGTTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:388
Protein Molecular Weight:43346
Protein Theoretical pI:7
Signaling Regions:
  • 1-21
Transmembrane Regions:
  • None
Protein Sequence:
>D-alanyl-D-alanine carboxypeptidase dacD
MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDYTTGQILTAGNEHQQRN
PASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSVRD
LSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLHLKDTHFETVHGLDAPGQHSS
AYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMNVDGLKTGHTSGAG
FNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLRWGQQNFTTVQILHRGKKVGTERIW
YGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLDGKELTAPISAHQRVGEIELYDRDKQV
AHWPLVTLESVGEGSMFSRLSDYFHHKA
References
External Links:
ResourceLink
Uniprot ID:P33013
Uniprot Name:DACD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321905
Ecogene ID:EG11893
Ecocyc:EG11893
ColiBase:b2010
Kegg Gene:b2010
EchoBASE ID:EB1839
CCDB:DACD_ECOLI
BacMap:162135904
General Reference:
  • Baquero, M. R., Bouzon, M., Quintela, J. C., Ayala, J. A., Moreno, F. (1996). "dacD, an Escherichia coli gene encoding a novel penicillin-binding protein (PBP6b) with DD-carboxypeptidase activity." J Bacteriol 178:7106-7111. Pubmed: 8955390
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040