ECMDB: Phosphoglycolate phosphatase (P32662)

Identification

Name:

Phosphoglycolate phosphatase

Synonyms:

PGP
PGPase

Gene Name:

gph

Enzyme Class:

3.1.3.18

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stress

Cellular Location:

Cytoplasmic

SMPDB Pathways:

Not Available

KEGG Pathways:

Glyoxylate and dicarboxylate metabolism ec00630
Metabolic pathways eco01100

KEGG Reactions:

1.0

↔

1.0

1.0Phosphoglycolic acid + 1.0Water ↔ 1.0Glycolic acid + 1.0Phosphate + 1.0Glycolate
ReactionCard

1.0

↔

1.0

1.0Phosphoglycolic acid + 1.0Water ↔ 1.0Glycolic acid + 1.0Phosphate
ReactionCard

1.0

→

1.0

1.0Water + 1.0Phosphoglycolic acid → 1.0Glycolic acid + 1.0Phosphate
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Phosphoglycolic acid + 1.0Water ↔ 1.0Glycolic acid + 1.0Phosphate + 1.0Glycolate
ReactionCard

1.0

→

1.0

1.0Water + 1.0Phosphoglycolic acid → 1.0Glycolic acid + 1.0Phosphate
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Phosphoglycolic acid + 1.0Water → 1.0Glycolic acid + 1.0Inorganic phosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB23741	Glycolate	MetaboCard
ECMDB03035	Glycolic acid	MetaboCard
ECMDB21380	Inorganic phosphate	MetaboCard
ECMDB01429	Phosphate	MetaboCard
ECMDB04130	Phosphoglycolic acid	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
phosphatase activity
phosphoglycolate phosphatase activity
phosphoric ester hydrolase activity
Process
metabolic process

Gene Properties

Blattner:

b3385

Gene Orientation

Counterclockwise

Centisome Percentage:

75.69

Left Sequence End

3511653

Right Sequence End

3512411

Gene Sequence:

>759 bp
ATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCG
GTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGT
GGCCCAGGTAACAGCACCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGC
ATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTC
GTGGAAGCAGACATCCGCAACTGGCAACCGGTACAGGCACTCGATCTGATTTTTGCTAAT
GCCTCACTGCAATGGCTGCCCGACCACTACGAATTGTTTCCTCATCTGGTTTCTTTACTT
AATCCGCAGGGCGTGCTGGCAGTACAGATGCCAGATAACTGGCTGGAGCCGACCCATGTG
CTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCT
GGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGA
ACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACT
GGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGC
TACCATCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCA
TTTCCGCGTCTGTTTATTGTTGCCCGGCGTATGGAGTAA

Protein Properties

Pfam Domain Function:

Hydrolase (PF00702 )

Protein Residues:

252

Protein Molecular Weight:

27389

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Phosphoglycolate phosphatase
MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMER
ALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTL
GALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGGDDVQNKKPHPDPLLLVAERM
GIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
LPHSENQESKND

References

External Links:

Resource	Link
Uniprot ID:	P32662
Uniprot Name:	GPH_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85675003
Ecogene ID:	EG11871
Ecocyc:	EG11871
ColiBase:	b3385
Kegg Gene:	b3385
EchoBASE ID:	EB1817
CCDB:	GPH_ECOLI
BacMap:	16131263

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Lyngstadaas, A., Lobner-Olesen, A., Boye, E. (1995). "Characterization of three genes in the dam-containing operon of Escherichia coli." Mol Gen Genet 247:546-554. Pubmed: 7603433
Lyngstadaas, A., Lobner-Olesen, A., Grelland, E., Boye, E. (1999). "The gene for 2-phosphoglycolate phosphatase (gph) in Escherichia coli is located in the same operon as dam and at least five other diverse genes." Biochim Biophys Acta 1472:376-384. Pubmed: 10572959
Teresa Pellicer, M., Felisa Nunez, M., Aguilar, J., Badia, J., Baldoma, L. (2003). "Role of 2-phosphoglycolate phosphatase of Escherichia coli in metabolism of the 2-phosphoglycolate formed in DNA repair." J Bacteriol 185:5815-5821. Pubmed: 13129953