| Identification |
|---|
| Name: | Transcription-repair-coupling factor |
|---|
| Synonyms: | Not Available |
|---|
| Gene Name: | mfd |
|---|
| Enzyme Class: | Not Available |
|---|
| Biological Properties |
|---|
| General Function: | transcription-coupled nucleotide-excision repair, DNA damage recognition |
|---|
| Specific Function: | Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site. Can also dissociate RNAP that is blocked by low concentration of nucleoside triphosphates or by physical obstruction, such as bound proteins. In addition, can rescue arrested complexes by promoting forward translocation. Has ATPase activity, which is required for removal of stalled RNAP, but seems to lack helicase activity. May act through a translocase activity that rewinds upstream DNA, leading either to translocation or to release of RNAP when the enzyme active site can not continue elongation. |
|---|
| Cellular Location: | Not Available |
|---|
| SMPDB Pathways: | Not Available |
|---|
| KEGG Pathways: | |
|---|
| Metabolites: | |
|---|
| GO Classification: | | Function |
|---|
| ATP binding | | ATP-dependent helicase activity | | cellular response to DNA damage stimulus | | cytoplasm | | damaged DNA binding | | DNA binding | | DNA repair | | regulation of transcription, DNA-templated | | transcription-coupled nucleotide-excision repair, DNA damage recognition |
|
|---|
| Gene Properties |
|---|
| Blattner: | Not Available |
|---|
| Gene Orientation | Not Available |
|---|
| Centisome Percentage: | Not Available |
|---|
| Left Sequence End | Not Available |
|---|
| Right Sequence End | Not Available |
|---|
| Gene Sequence: | >3447
atgcctgaacaatatcgttatacgctgcccgtcaaagcgggtgagcagcgtctgctgggc
gagttaaccggcgcagcctgtgcaacgctggtagcggaaattgccgaacgtcacgccggt
ccggtggtactcattgcaccagatatgcaaaatgctctgcgtttgcatgatgaaatcagc
cagtttaccgatcaaatggtgatgaatctggcggactgggaaactcttccctacgacagt
ttttcgcctcatcaggacattatctcctcgcgcctttccaccctttaccagctaccgacg
atgcagcgtggcgtactgattgttccggtgaatacgcttatgcagcgcgtttgcccacac
agttttctccacggtcatgcgctggtgatgaaaaaaggtcagcgcctgtcacgagatgca
ttacgaacccaactggacagcgccggttatcgccatgttgaccaggtgatggagcacggc
gaatacgccacgcgcggcgcgttgctggatctcttcccgatggggagtgagctgccttat
cgtcttgatttctttgatgatgaaatcgacagcctgcgggtgtttgacgtcgacagccag
cgcacgctggaggaagtagaagcgatcaatctgctgcccgcgcacgaatttccgaccgat
aaagcggcaattgaactgttccgcagccagtggcgcgataccttcgaagtgaagcgcgat
ccagaacatatttaccagcaagtgagtaaaggcacattacctgccgggatcgagtactgg
cagccattgttcttcagcgaaccactgccgccgctgttcagttatttccctgccaatacc
ttgctcgtgaatactggcgatctggaaaccagtgccgaacgtttccaggctgacacgctg
gcgcgttttgagaatcgcggcgtcgatccgatgcgcccgctgttgccaccacaatcgctc
tggctgcgggtggacgagctcttctcagagctgaaaaactggccccgggtgcagctaaaa
actgaacatttaccgacaaaagccgcgaatgccaatttaggtttccagaaactgccagac
ctggccgttcaggcacaacaaaaagcgccgctggatgcgctgcgtaagttcctcgagact
ttcgacggtccggtggtgttctcggtagaaagtgaaggtcgccgtgaagcgctgggtgaa
ctgctcgcacgaattaaaattgctccgcaacgcattatgcgtcttgatgaagccagcgac
cgtgggcgttatctgatgattggcgctgccgaacatggttttgtcgatacggtgcgtaat
ctggcgctgatttgcgaaagcgatctgctcggtgaacgcgttgcccgtcgtcgtcaggat
tctcgccgcaccatcaaccccgatacactgatccgtaaccttgcggaactgcatattggt
cagccggtggtccatctggagcacggcgtcggtcgttatgccggaatgaccacgctggaa
gcgggtggcattactggcgagtatctgatgctcacctatgccaacgacgccaaactgtat
gttccggtgtcgtcactgcatctgattagccgttacgcaggtggcgcggaagaaaacgcc
ccgctgcataaacttggcggcgatgcgtggtcacgcgcgcggcagaaagcggcggaaaaa
gtgcgtgatgtggcggcggaattgctggatatctacgcgcaacgcgccgccaaagagggc
ttcgcgtttaaacacgatcgtgagcagtatcagttgttctgcgacagcttcccgtttgaa
accacgccggatcaggcgcaggccattaatgcggtacttagcgacatgtgtcagccgctg
gcaatggatcgtctggtgtgcggcgatgttggctttggtaaaacagaagtggcgatgcgc
gcagctttcctggcagtagataaccacaagcaggtggcggtgctggtgcctaccaccctt
ctcgcgcagcagcattacgacaacttccgcgaccgtttcgccaactggccggtacgtatc
gaaatgatctcccgtttccgcagcgccaaagagcagacgcaaatccttgcggaagtggcg
gaagggaaaatcgatattctgatcggtacgcacaaactgctgcaaagtgacgtcaagttt
aaagatttaggcctgctgattgtcgatgaagaacaccgcttcggggtgcgtcataaagag
cgcattaaagcgatgcgcgcgaacgtggatattctgacgcttactgcaacgccgatccca
cgtacgctgaatatggcaatgagcggaatgcgtgacctgtcgattatcgccacgccgccc
gcccgtcgtctggcagttaaaacctttgtccgtgagtatgacagcatggtggtccgggag
gcgatcctgcgtgaaattttgcgcggaggacaggtttattatctctacaatgatgtggaa
aacattcagaaagccgccgaacggctggcagaactggtgccagaagcgcggatcgccatc
ggtcacgggcagatgcgcgagcgcgaactggaacgggtgatgaatgatttccatcatcaa
cgtttcaacgtgctggtttgtacaaccattatcgaaaccgggatcgacatcccgacagcc
aacactattatcattgaacgcgcggatcacttcggtctggcgcagctgcaccagttacgc
ggtcgcgtcggacgttcgcatcatcaggcatatgcatggttgctgacaccgcatccaaaa
gcgatgactaccgatgcacaaaaacgtcttgaagcaattgcctcgctggaagatctcggg
gcaggttttgcgctggcaacgcacgatctggagattcgcggcgcgggtgaactgcttggc
gaagaacaaagcggctcaatggaaaccatcggtttctcgctgtatatggagttgctggaa
aacgccgtcgatgcactgaaagccggacgcgagccgtcgctggaagatctcaccagccag
caaacagaagtcgagctgcggatgccgtcgctattgccagatgatttcatccctgacgtg
aacacgcgtctgtcgttctacaaacgtattgccagcgccaaaacggaaaacgaactggaa
gagatcaaagtcgagcttatcgatcgcttcggcctgctgccagatccggcgcgtaccctg
ctggatattgcccgtctgcgccagcaagcgcagaaactggggatcaggaagctggaaggt
aatgagaaaggcggggtgatcgaatttgccgagaagaatcacgttaatccggcctggttg
attggtttgctgcaaaaacagccgcagcattaccgccttgatggtccgacgcgcctgaaa
tttattcaggatttgagtgagcggaaaacgcgtatcgaatgggtacgccagtttatgcgt
gaactggaagagaacgcgatcgcttaa |
|---|
| Protein Properties |
|---|
| Pfam Domain Function: | Not Available |
|---|
| Protein Residues: | 1148 |
|---|
| Protein Molecular Weight: | 129981 |
|---|
| Protein Theoretical pI: | Not Available |
|---|
| PDB File: | 2B2N |
| Signaling Regions: | Not Available |
|---|
| Transmembrane Regions: | Not Available |
|---|
| Protein Sequence: | >Transcription-repair-coupling factor
MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVLIAPDMQNALRLHDEIS
QFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQRGVLIVPVNTLMQRVCPH
SFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHGEYATRGALLDLFPMGSELPY
RLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFPTDKAAIELFRSQWRDTFEVKRD
PEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYFPANTLLVNTGDLETSAERFQADTL
ARFENRGVDPMRPLLPPQSLWLRVDELFSELKNWPRVQLKTEHLPTKAANANLGFQKLPD
LAVQAQQKAPLDALRKFLETFDGPVVFSVESEGRREALGELLARIKIAPQRIMRLDEASD
RGRYLMIGAAEHGFVDTVRNLALICESDLLGERVARRRQDSRRTINPDTLIRNLAELHIG
QPVVHLEHGVGRYAGMTTLEAGGITGEYLMLTYANDAKLYVPVSSLHLISRYAGGAEENA
PLHKLGGDAWSRARQKAAEKVRDVAAELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFE
TTPDQAQAINAVLSDMCQPLAMDRLVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTL
LAQQHYDNFRDRFANWPVRIEMISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKF
KDLGLLIVDEEHRFGVRHKERIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPP
ARRLAVKTFVREYDSMVVREAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAI
GHGQMRERELERVMNDFHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLR
GRVGRSHHQAYAWLLTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLG
EEQSGSMETIGFSLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDV
NTRLSFYKRIASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEG
NEKGGVIEFAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMR
ELEENAIA |
|---|
| References |
|---|
| External Links: | |
|---|
| General Reference: | Not Available |
|---|
