o-succinylbenzoate synthase (P29208)

Identification
Name:o-succinylbenzoate synthase
Synonyms:
  • OSB synthase
  • OSBS
  • 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase
  • o-succinylbenzoic acid synthase
Gene Name:menC
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Converts SHCHC to OSB
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Metabolic pathways eco01100
  • Ubiquinone and other terpenoid-quinone biosynthesis ec00130
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate ↔ 1.0Water + 1.02-Succinylbenzoate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.02-Succinylbenzoate + 1.0Water ↔ 1.0(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.0(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate → 1.02-Succinylbenzoate + 1.0Water
ReactionCard
SMPDB Reactions:
1.0(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate+1.0Thumb → 1.02-succinylbenzoate+1.0Thumb+1.0Thumb
1.0(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + 1.0(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate → 1.02-succinylbenzoate + 1.0Water + 1.02-Succinylbenzoate
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate ↔ 1.0Water + 1.02-Succinylbenzoate
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.0(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate → 1.02-Succinylbenzoate + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB20002(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylateMetaboCard
ECMDB200512-SuccinylbenzoateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
binding
carbon-oxygen lyase activity
catalytic activity
cation binding
hydro-lyase activity
ion binding
lyase activity
magnesium ion binding
metal ion binding
Process
fat-soluble vitamin metabolic process
menaquinone biosynthetic process
menaquinone metabolic process
metabolic process
small molecule metabolic process
vitamin K metabolic process
vitamin metabolic process
Gene Properties
Blattner:b2261
Gene OrientationCounterclockwise
Centisome Percentage:51.15
Left Sequence End2373022
Right Sequence End2373984
Gene Sequence:
>963 bp
ATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCA
CAGGCGGTGAACGTCACCGTGGCGTATCAAACCTCAGCCGAACCGGCGAAAGTGGCTCAG
GCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGC
GGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCC
AGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCA
AAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCAGCAAACCGGAAGATCTG
ATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCA
CTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCG
ATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTT
AACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGC
GCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTC
GTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGAC
GTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAA
GGTGACGTTCCGGGGCTGGTGAAGGGGAATACCTATCTGACGCCGCAGCAACAAACGGCA
GAACTGACCGGACCGGTGAACAAAGCGATCATCGACACCGCGCAGTTTTTGAAAGAGCAG
GGCAAGGTCCCGGCTGTAGCGAATGATTACAGCCAGTACGTTACCTCGCGCTTCGTGCAA
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:320
Protein Molecular Weight:35476
Protein Theoretical pI:5
PDB File:1FHU
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>o-succinylbenzoate synthase
MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGEISPLPGFSQETWEEAQ
SVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAANYRAAPLCNGDPDDLILK
LADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRLDANRAWTPLKGQQFAKYVNP
DYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLREPDFAFVAEEGVRAVVIKPTLTG
SLEKVREQVQAAHALGLTAVISSSIESSLGLTQLARIAAWLTPDTIPGLDTLDLMQAQQV
RRWPGSTLPVVEVDALERLL
References
External Links:
ResourceLink
Uniprot ID:P29208
Uniprot Name:MENC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674506
PDB ID:1FHU
Ecogene ID:EG11532
Ecocyc:EG11532
ColiBase:b2261
Kegg Gene:b2261
EchoBASE ID:EB1494
CCDB:MENC_ECOLI
BacMap:16130196
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Palmer, D. R., Garrett, J. B., Sharma, V., Meganathan, R., Babbitt, P. C., Gerlt, J. A. (1999). "Unexpected divergence of enzyme function and sequence: "N-acylamino acid racemase" is o-succinylbenzoate synthase." Biochemistry 38:4252-4258. Pubmed: 10194342
  • Sharma, V., Meganathan, R., Hudspeth, M. E. (1993). "Menaquinone (vitamin K2) biosynthesis: cloning, nucleotide sequence, and expression of the menC gene from Escherichia coli." J Bacteriol 175:4917-4921. Pubmed: 8335646
  • Thompson, T. B., Garrett, J. B., Taylor, E. A., Meganathan, R., Gerlt, J. A., Rayment, I. (2000). "Evolution of enzymatic activity in the enolase superfamily: structure of o-succinylbenzoate synthase from Escherichia coli in complex with Mg2+ and o-succinylbenzoate." Biochemistry 39:10662-10676. Pubmed: 10978150
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837