tRNA-specific 2-thiouridylase mnmA (P25745)

Identification
Name:tRNA-specific 2-thiouridylase mnmA
Synonyms:Not Available
Gene Name:mnmA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in sulfurtransferase activity
Specific Function:Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by iscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:
EcoCyc Reactions:
1.0a tRNA uridine34+1.0a [TusE sulfur carrier protein]-S-sulfanylcysteine+1.0Thumb → 1.0a tRNA 2-thiouridine34+1.0a [TusE sulfur carrier protein]-L-cysteine+1.0Thumb+1.0Thumb
1.0a tRNA uridine34 + 1.0a [TusE sulfur carrier protein]-S-sulfanylcysteine + 1.0Adenosine triphosphate → 1.0a tRNA 2-thiouridine34 + 1.0a [TusE sulfur carrier protein]-L-cysteine + 1.0Adenosine monophosphate + 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
transferase activity
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
tRNA metabolic process
tRNA processing
Gene Properties
Blattner:b1133
Gene OrientationCounterclockwise
Centisome Percentage:25.69
Left Sequence End1191890
Right Sequence End1192996
Gene Sequence:
>1107 bp
ATGCTCAGTCAAATTCAACGCTTTGGCGGCGCGATGTTCACGCCAGTGCTGCTGTTTCCC
TTCGCCGGGATTGTGGTGGGTCTTGCCATCTTGCTGCAAAACCCGATGTTTGTCGGGGAA
TCACTGACCGATCCGAACAGTTTATTCGCGCAAATCGTACACATTATTGAAGAGGGCGGT
TGGACGGTATTCCGTAATATGCCGCTGATTTTTGCTGTCGGTTTACCCATTGGCCTTGCT
AAGCAAGCGCAGGGGCGTGCTTGTCTGGCGGTGATGGTGAGTTTCCTGACCTGGAACTAT
TTCATCAACGCGATGGGAATGACCTGGGGAAGCTACTTCGGCGTCGATTTCACTCAGGAC
GCGGTGGCAGGTAGCGGTCTGACAATGATGGCCGGGATTAAAACCCTCGATACCAGCATT
ATCGGCGCAATTATCATTTCCGGCATTGTGACGGCGCTGCATAACCGTCTGTTCGATAAA
AAACTGCCGGTTTTTCTCGGCATTTTCCAGGGGACGTCTTATGTGGTGATTATCGCCTTC
CTGGTGATGATCCCCTGTGCCTGGCTGACGTTGCTCGGCTGGCCAAAAGTACAAATGGGG
ATTGAATCTCTGCAAGCGTTCCTGCGTTCGGCGGGTGCACTTGGGGTCTGGGTTTACACC
TTCCTCGAACGTATTCTGATCCCAACCGGTTTACACCACTTCATCTACGGACAGTTTATC
TTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAG
TTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCAC
GGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTAGCGATGTACTTCACTGCCGCA
CCGGAAAATCGGGTAAAAGTGGCGGGCTTGCTGATTCCCGCAACCTTAACCGCCATGCTG
GTGGGAATTACCGAACCGCTGGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCG
GTACACGCCGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGC
AACATGGGCGGAGGTCTGATTGACTAG
Protein Properties
Pfam Domain Function:
Protein Residues:368
Protein Molecular Weight:40959
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>tRNA-specific 2-thiouridylase mnmA
MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDDGEEYCTAAADLADAQA
VCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILCNKEIKFKAFLEFAAEDLG
ADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSHEQIAQSLFPVGELEKPQVRK
IAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQPGKIITVDGDEIGEHQGLMYHTL
GQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQGHEHPRLMSVGLIAQQLHWVDREPF
TGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFDEPVAAVTPGQSAVFYNGEVCLGGGII
EQRLPLPV
References
External Links:
ResourceLink
Uniprot ID:P25745
Uniprot Name:MNMA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676361
Ecogene ID:EG11344
Ecocyc:EG11344
ColiBase:b1133
Kegg Gene:b1133
EchoBASE ID:EB1320
CCDB:MNMA_ECOLI
BacMap:90111218
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Green, S. M., Malik, T., Giles, I. G., Drabble, W. T. (1996). "The purB gene of Escherichia coli K-12 is located in an operon." Microbiology 142 ( Pt 11):3219-3230. Pubmed: 8969519
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • He, B., Smith, J. M., Zalkin, H. (1992). "Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR." J Bacteriol 174:130-136. Pubmed: 1729205
  • Ikeuchi, Y., Shigi, N., Kato, J., Nishimura, A., Suzuki, T. (2006). "Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions." Mol Cell 21:97-108. Pubmed: 16387657
  • Kambampati, R., Lauhon, C. T. (2003). "MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli." Biochemistry 42:1109-1117. Pubmed: 12549933
  • Numata, T., Ikeuchi, Y., Fukai, S., Adachi, H., Matsumura, H., Takano, K., Murakami, S., Inoue, T., Mori, Y., Sasaki, T., Suzuki, T., Nureki, O. (2006). "Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNA(Glu)." Acta Crystallogr Sect F Struct Biol Cryst Commun 62:368-371. Pubmed: 16582487
  • Numata, T., Ikeuchi, Y., Fukai, S., Suzuki, T., Nureki, O. (2006). "Snapshots of tRNA sulphuration via an adenylated intermediate." Nature 442:419-424. Pubmed: 16871210
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Sullivan, M. A., Cannon, J. F., Webb, F. H., Bock, R. M. (1985). "Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine." J Bacteriol 161:368-376. Pubmed: 3881393