Alpha-amylase (P25718)

Identification
Name:Alpha-amylase
Synonyms:
  • 1,4-alpha-D-glucan glucanohydrolase
Gene Name:malS
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, malF, malG, and malK, longer maltooligosaccharides must first be degraded by the periplasmic alpha-amylase, the malS protein
Cellular Location:Periplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Starch+1.0Thumb ↔ 1.0Thumb+1.0Starch
1.0Starch + 1.0Water ↔ 1.0Dextrin + 1.0Starch
ReactionCard
EcoCyc Reactions:
1.0a 1,4-α-D-glucan+1.0Thumb → 1.0a 1,4-α-D-glucan+1.0Thumb
1.0a 1,4-α-D-glucan + 1.0Water → 1.0a 1,4-α-D-glucan + 1.0Maltohexaose
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211101,4-alpha-D-glucanMetaboCard
ECMDB21651DextrinMetaboCard
ECMDB21243MaltohexaoseMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
ion binding
Process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b3571
Gene OrientationClockwise
Centisome Percentage:80.51
Left Sequence End3735520
Right Sequence End3737550
Gene Sequence:
>2031 bp
ATGAAACTCGCCGCCTGTTTTCTGACACTCCTTCCTGGCTTCGCCGTTGCCGCCAGCTGG
ACTTCTCCGGGGTTTCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCG
CAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCT
GCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCT
CAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCGCTCCGGTACG
CCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAA
TGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGA
GCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATG
TTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACA
TCCGCCCCTTTCGACTGGCATAACGCCACGGTTTACTTTGTGCTGACAGATCGTTTCGAA
AACGGCGATCCCAGTAATGACCAGAGTTACGGACGTCATAAAGACGGTATGGCGGAAATT
GGCACTTTTCACGGCGGCGATTTACGCGGCCTGACCAACAAACTGGATTACCTCCAGCAG
TTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGC
GGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGG
ACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCA
CATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACG
CTGGCGGATATGCAGGAGTATCAGTTTGGCGCGTTATATCTTTCTGGTGACGAAGTGAAA
AAATCGCTGGGTGAACGCTGGAGCGACTGGAAACCTGCCGCCGGGCAAACCTGGCATAGC
TTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAAC
TGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCG
CTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTC
TATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTAC
TTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGAT
ACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCG
CTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATG
ACCGGTGAAGCCTGGGGCCACGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTCGAT
GCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAG
ATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTAC
CTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTA
CTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGT
CCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGC
GGTAAATCTGCCGCCAGCGTCGCGCACTGGCAGAAAATCAGCCAGTTCCGCGCCCGCCAT
CCCGCAATTGGCGCGGGCAAACAAACGACACTTTTGCTGAAGCAGGGCTACGGCTTTGTT
CGTGAGCATGGCGACGATAAAGTGCTGGTCGTCTGGGCAGGGCAACAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:676
Protein Molecular Weight:75712
Protein Theoretical pI:5
Signaling Regions:
  • 1-17
Transmembrane Regions:
  • None
Protein Sequence:
>Alpha-amylase
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQP
ADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPTLMISIQNAAEPVASLVRE
CPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIMLQPAATSNGLLLLERAETDT
SAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSA
HQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDEVKKSLGERWSDWKPAAGQTWHS
FNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVF
YKNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQ
MDTTWQQMAEKLQGFNVLSYLSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFG
PTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGAGKQTTLLLKQGYGFV
REHGDDKVLVVWAGQQ
References
External Links:
ResourceLink
Uniprot ID:P25718
Uniprot Name:AMY1_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676472
Ecogene ID:EG11316
Ecocyc:EG11316
ColiBase:b3571
Kegg Gene:b3571
EchoBASE ID:EB1292
CCDB:AMY1_ECOLI
BacMap:16131442
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Schneider, E., Freundlieb, S., Tapio, S., Boos, W. (1992). "Molecular characterization of the MalT-dependent periplasmic alpha-amylase of Escherichia coli encoded by malS." J Biol Chem 267:5148-5154. Pubmed: 1544897
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
  • Spiess, C., Happersberger, H. P., Glocker, M. O., Spiess, E., Rippe, K., Ehrmann, M. (1997). "Biochemical characterization and mass spectrometric disulfide bond mapping of periplasmic alpha-amylase MalS of Escherichia coli." J Biol Chem 272:22125-22133. Pubmed: 9268356