| Identification |
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| Name: | Alpha-amylase |
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| Synonyms: | - 1,4-alpha-D-glucan glucanohydrolase
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| Gene Name: | malS |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | Involved in catalytic activity |
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| Specific Function: | Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, malF, malG, and malK, longer maltooligosaccharides must first be degraded by the periplasmic alpha-amylase, the malS protein |
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| Cellular Location: | Periplasm |
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| SMPDB Pathways: | |
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| KEGG Pathways: | |
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| KEGG Reactions: | |
1.0Starch | + | 1.0 | ↔ | 1.0 | + | 1.0Starch |
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| EcoCyc Reactions: | |
1.0a 1,4-α-D-glucan | + | 1.0 | → | 1.0a 1,4-α-D-glucan | + | 1.0 |
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| Metabolites: | |
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| GO Classification: | | Function |
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| binding | | catalytic activity | | cation binding | | ion binding | | Process |
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| carbohydrate metabolic process | | metabolic process | | primary metabolic process |
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| Gene Properties |
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| Blattner: | b3571 |
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| Gene Orientation | Clockwise |
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| Centisome Percentage: | 80.51 |
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| Left Sequence End | 3735520 |
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| Right Sequence End | 3737550 |
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| Gene Sequence: | >2031 bp
ATGAAACTCGCCGCCTGTTTTCTGACACTCCTTCCTGGCTTCGCCGTTGCCGCCAGCTGG
ACTTCTCCGGGGTTTCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCG
CAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCT
GCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCT
CAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCGCTCCGGTACG
CCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAA
TGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGA
GCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATG
TTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACA
TCCGCCCCTTTCGACTGGCATAACGCCACGGTTTACTTTGTGCTGACAGATCGTTTCGAA
AACGGCGATCCCAGTAATGACCAGAGTTACGGACGTCATAAAGACGGTATGGCGGAAATT
GGCACTTTTCACGGCGGCGATTTACGCGGCCTGACCAACAAACTGGATTACCTCCAGCAG
TTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGC
GGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGG
ACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCA
CATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACG
CTGGCGGATATGCAGGAGTATCAGTTTGGCGCGTTATATCTTTCTGGTGACGAAGTGAAA
AAATCGCTGGGTGAACGCTGGAGCGACTGGAAACCTGCCGCCGGGCAAACCTGGCATAGC
TTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAAC
TGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCG
CTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTC
TATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTAC
TTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTTCGGGTCGAT
ACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCG
CTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATG
ACCGGTGAAGCCTGGGGCCACGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTCGAT
GCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAG
ATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTAC
CTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTA
CTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGT
CCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGC
GGTAAATCTGCCGCCAGCGTCGCGCACTGGCAGAAAATCAGCCAGTTCCGCGCCCGCCAT
CCCGCAATTGGCGCGGGCAAACAAACGACACTTTTGCTGAAGCAGGGCTACGGCTTTGTT
CGTGAGCATGGCGACGATAAAGTGCTGGTCGTCTGGGCAGGGCAACAGTAA |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 676 |
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| Protein Molecular Weight: | 75712 |
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| Protein Theoretical pI: | 5 |
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| Signaling Regions: | |
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| Transmembrane Regions: | |
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| Protein Sequence: | >Alpha-amylase
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQP
ADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPTLMISIQNAAEPVASLVRE
CPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIMLQPAATSNGLLLLERAETDT
SAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSA
HQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDEVKKSLGERWSDWKPAAGQTWHS
FNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVF
YKNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQ
MDTTWQQMAEKLQGFNVLSYLSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFG
PTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGAGKQTTLLLKQGYGFV
REHGDDKVLVVWAGQQ |
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| References |
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| External Links: | |
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| General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Schneider, E., Freundlieb, S., Tapio, S., Boos, W. (1992). "Molecular characterization of the MalT-dependent periplasmic alpha-amylase of Escherichia coli encoded by malS." J Biol Chem 267:5148-5154. Pubmed: 1544897
- Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
- Spiess, C., Happersberger, H. P., Glocker, M. O., Spiess, E., Rippe, K., Ehrmann, M. (1997). "Biochemical characterization and mass spectrometric disulfide bond mapping of periplasmic alpha-amylase MalS of Escherichia coli." J Biol Chem 272:22125-22133. Pubmed: 9268356
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