ECMDB

Identification

Name:

Geranyltranstransferase

Synonyms:

Farnesyl-diphosphate synthase
FPP synthase

Gene Name:

ispA

Enzyme Class:

2.5.1.10

Biological Properties

General Function:

Involved in isoprenoid biosynthetic process

Specific Function:

Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate

Cellular Location:

Cytoplasm

SMPDB Pathways:

Secondary metabolites: methylerythritol phosphate and polyisoprenoid biosynthesis PW000958

KEGG Pathways:

Metabolic pathways eco01100
Terpenoid backbone biosynthesis ec00900

KEGG Reactions:

1.0

→

1.0

1.0Dimethylallylpyrophosphate + 1.0Isopentenyl pyrophosphate → 1.0Geranyl-PP + 1.0Pyrophosphate
ReactionCard

1.0

↔

1.0

1.0Geranyl-PP + 1.0Isopentenyl pyrophosphate + 1.0Geranyl diphosphate ↔ 1.0Farnesyl pyrophosphate + 1.0Pyrophosphate
ReactionCard

1.0

↔

1.0

1.0Dimethylallylpyrophosphate + 1.0Isopentenyl pyrophosphate ↔ 1.0Pyrophosphate + 1.0Geranyl-PP
ReactionCard

1.0

↔

1.0

1.0Geranyl-PP + 1.0Isopentenyl pyrophosphate ↔ 1.0Pyrophosphate + 1.0Farnesyl pyrophosphate
ReactionCard

SMPDB Reactions:

1.0Dimethylallylpyrophosphate

1.0Isopentenyl pyrophosphate

1.0

→

1.0Pyrophosphate

1.0Geranyl-PP

1.0

1.0Dimethylallylpyrophosphate + 1.0Isopentenyl pyrophosphate + 1.0Dimethylallylpyrophosphate + 1.0Isopentenyl pyrophosphate → 1.0Pyrophosphate + 1.0Geranyl-PP + 1.0Geranyl-PP
ReactionCard

1.0Geranyl-PP

1.0Isopentenyl pyrophosphate

1.0

→

1.0Pyrophosphate

1.0Farnesyl pyrophosphate

1.0

1.0Geranyl-PP + 1.0Isopentenyl pyrophosphate + 1.0Geranyl-PP + 1.0Isopentenyl pyrophosphate → 1.0Pyrophosphate + 1.0Farnesyl pyrophosphate + 1.0Farnesyl pyrophosphate
ReactionCard

EcoCyc Reactions:

1.0

→

1.0

1.0Dimethylallylpyrophosphate + 1.0Isopentenyl pyrophosphate → 1.0Geranyl-PP + 1.0Pyrophosphate
ReactionCard

1.0

↔

1.0

1.0Geranyl-PP + 1.0Isopentenyl pyrophosphate + 1.0Geranyl diphosphate ↔ 1.0Farnesyl pyrophosphate + 1.0Pyrophosphate
ReactionCard

1.0

↔

1.0

1.0Dimethylallylpyrophosphate + 1.0Isopentenyl pyrophosphate ↔ 1.0Pyrophosphate + 1.0Geranyl-PP
ReactionCard

1.0

↔

1.0

1.0Geranyl-PP + 1.0Isopentenyl pyrophosphate ↔ 1.0Pyrophosphate + 1.0Farnesyl pyrophosphate
ReactionCard

1.0

→

1.0

1.0Geranyl-PP + 1.0Isopentenyl pyrophosphate → 1.0Farnesyl pyrophosphate + 1.0Pyrophosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01120	Dimethylallylpyrophosphate	MetaboCard
ECMDB00961	Farnesyl pyrophosphate	MetaboCard
ECMDB23137	Geranyl diphosphate	MetaboCard
ECMDB01285	Geranyl-PP	MetaboCard
ECMDB01347	Isopentenyl pyrophosphate	MetaboCard
ECMDB04142	Pyrophosphate	MetaboCard

GO Classification:

Process
cellular lipid metabolic process
isoprenoid biosynthetic process
isoprenoid metabolic process
lipid metabolic process
metabolic process
primary metabolic process

Gene Properties

Blattner:

b0421

Gene Orientation

Counterclockwise

Centisome Percentage:

9.47

Left Sequence End

439426

Right Sequence End

440325

Gene Sequence:

>900 bp
ATGGACTTTCCGCAGCAACTCGAAGCCTGCGTTAAGCAGGCCAACCAGGCGCTGAGCCGT
TTTATCGCCCCACTGCCCTTTCAGAACACTCCCGTGGTCGAAACCATGCAGTATGGCGCA
TTATTAGGTGGTAAGCGCCTGCGACCTTTCCTGGTTTATGCCACCGGTCATATGTTCGGC
GTTAGCACAAACACGCTGGACGCACCCGCTGCCGCCGTTGAGTGTATCCACGCTTACTCA
TTAATTCATGATGATTTACCGGCAATGGATGATGACGATCTGCGTCGCGGTTTGCCAACC
TGCCATGTGAAGTTTGGCGAAGCAAACGCGATTCTCGCTGGCGACGCTTTACAAACGCTG
GCGTTCTCGATTTTAAGCGATGCCGATATGCCGGAAGTGTCGGACCGCGACAGAATTTCG
ATGATTTCTGAACTGGCGAGCGCCAGTGGTATTGCCGGAATGTGCGGTGGTCAGGCATTA
GATTTAGACGCGGAAGGCAAACACGTACCTCTGGACGCGCTTGAGCGTATTCATCGTCAT
AAAACCGGCGCATTGATTCGCGCCGCCGTTCGCCTTGGTGCATTAAGCGCCGGAGATAAA
GGACGTCGTGCTCTGCCGGTACTCGACAAGTATGCAGAGAGCATCGGCCTTGCCTTCCAG
GTTCAGGATGACATCCTGGATGTGGTGGGAGATACTGCAACGTTGGGAAAACGCCAGGGT
GCCGACCAGCAACTTGGTAAAAGTACCTACCCTGCACTTCTGGGTCTTGAGCAAGCCCGG
AAGAAAGCCCGGGATCTGATCGACGATGCCCGTCAGTCGCTGAAACAACTGGCTGAACAG
TCACTCGATACCTCGGCACTGGAAGCGCTAGCGGACTACATCATCCAGCGTAATAAATAA

Protein Properties

Pfam Domain Function:

polyprenyl_synt (PF00348 )

Protein Residues:

299

Protein Molecular Weight:

32159

Protein Theoretical pI:

PDB File:

1RQJ

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Geranyltranstransferase
MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGGKRLRPFLVYATGHMFG
VSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCHVKFGEANAILAGDALQTL
AFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQALDLDAEGKHVPLDALERIHRH
KTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLAFQVQDDILDVVGDTATLGKRQG
ADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQLAEQSLDTSALEALADYIIQRNK

References

External Links:

Resource	Link
Uniprot ID:	P22939
Uniprot Name:	ISPA_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674561
PDB ID:	1RQJ
Ecogene ID:	EG10508
Ecocyc:	EG10508
ColiBase:	b0421
Kegg Gene:	b0421
EchoBASE ID:	EB0503
CCDB:	ISPA_ECOLI
BacMap:	16128406

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Fujisaki, S., Hara, H., Nishimura, Y., Horiuchi, K., Nishino, T. (1990). "Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli." J Biochem 108:995-1000. Pubmed: 2089044
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Hosfield, D. J., Zhang, Y., Dougan, D. R., Broun, A., Tari, L. W., Swanson, R. V., Finn, J. (2004). "Structural basis for bisphosphonate-mediated inhibition of isoprenoid biosynthesis." J Biol Chem 279:8526-8529. Pubmed: 14672944

Geranyltranstransferase (P22939)