4-aminobutyrate aminotransferase (P22256)

Identification
Name:4-aminobutyrate aminotransferase
Synonyms:
  • (S)-3-amino-2-methylpropionate transaminase
  • GABA aminotransferase
  • GABA-AT
  • Gamma-amino-N-butyrate transaminase
  • GABA transaminase
  • Glutamate:succinic semialdehyde transaminase
  • L-AIBAT
Gene Name:gabT
Enzyme Class:
Biological Properties
General Function:Involved in 4-aminobutyrate transaminase activity
Specific Function:4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0gamma-Aminobutyric acid + 1.0alpha-Ketoglutarate ↔ 1.0L-Glutamate + 1.0Succinic acid semialdehyde
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0beta-Alanine + 1.0alpha-Ketoglutarate ↔ 1.0Malonic semialdehyde + 1.0L-Glutamate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0(S)-b-aminoisobutyric acid + 1.0alpha-Ketoglutarate ↔ 1.0(S)-Methylmalonic acid semialdehyde + 1.0L-Glutamate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0L-Glutamic acid+1.0Thumb
1.0gamma-Aminobutyric acid + 1.0Oxoglutaric acid → 1.0Succinic acid semialdehyde + 1.0L-Glutamic acid + 1.0L-Glutamate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0gamma-Aminobutyric acid + 1.0Oxoglutaric acid → 1.0Succinic acid semialdehyde + 1.0L-Glutamate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0(S)-b-aminoisobutyric acid + 1.0Oxoglutaric acid → 1.02-Methyl-3-oxopropanoate + 1.0L-Glutamate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB02166(S)-b-aminoisobutyric acidMetaboCard
ECMDB02217(S)-Methylmalonic acid semialdehydeMetaboCard
ECMDB231262-Methyl-3-oxopropanoateMetaboCard
ECMDB02812alpha-KetoglutarateMetaboCard
ECMDB00056beta-AlanineMetaboCard
ECMDB04017gamma-Aminobutyric acidMetaboCard
ECMDB00148L-GlutamateMetaboCard
ECMDB03015Malonic semialdehydeMetaboCard
ECMDB04123Oxoglutaric acidMetaboCard
ECMDB01259Succinic acid semialdehydeMetaboCard
GO Classification:
Function
4-aminobutyrate transaminase activity
binding
catalytic activity
cofactor binding
pyridoxal phosphate binding
transaminase activity
transferase activity
transferase activity, transferring nitrogenous groups
Process
cellular amino acid and derivative metabolic process
cellular amino acid derivative metabolic process
cellular metabolic process
gamma-aminobutyric acid metabolic process
metabolic process
Gene Properties
Blattner:b2662
Gene OrientationClockwise
Centisome Percentage:60.15
Left Sequence End2790757
Right Sequence End2792037
Gene Sequence:
>1281 bp
ATGACCGAACATACCAGCAGTTACTACGCCGCCAGTGCGAATAAATATGCACCATTCGAC
ACGCTGAATGAGTCAATCACCTGCGACGTTTGCGTGGTTGGCGGCGGCTATACCGGGCTC
TCCTCCGCGCTGCATCTGGCGGAAGCGGGCTTTGACGTAGTGGTTCTCGAAGCCTCACGC
ATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGAC
ATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATG
TTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTAC
CGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAG
AAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATT
CGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCAT
ATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGC
GTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACT
GCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGAT
AAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACC
GAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGAC
TGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGC
GGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTG
CTGAAAACCTTCCCGCAGCTGAAGGGCGTGAAAATTGATTACCGCTGGACGGGCAACTTC
CTGCTGACCCTGTCGCGTATGCCGCAGTTTGGTCGCCTCGATACCAACATCTATTACATG
CAGGGCTACAGCGGCCACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAA
CTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTC
CCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTG
CGCGATCGTCTGGGCGTTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:426
Protein Molecular Weight:45774
Protein Theoretical pI:6
PDB File:1SFF
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>4-aminobutyrate aminotransferase
MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYLDFAGGIAVLNTGHLHP
KVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFAKKTLLVTTGSEAVENAVK
IARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMGLMPGHVYRALYPCPLHGISE
DDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASSPAFMQRLRALCDEHGIMLIADE
VQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPLAGVTGRAEVMDAVAPGGLGGTYAG
NPIACVAALEVLKVFEQENLLQKANDLGQKLKDGLLAIAEKHPEIGDVRGLGAMIAIELF
EDGDHNKPDAKLTAEIVARARDKGLILLSCGPYYNVLRILVPLTIEDAQIRQGLEIISQC
FDEAKQ
References
External Links:
ResourceLink
Uniprot ID:P22256
Uniprot Name:GABT_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742131
PDB ID:1SFF
Ecogene ID:EG10361
Ecocyc:EG10361
ColiBase:b2662
Kegg Gene:b2662
EchoBASE ID:EB0356
CCDB:GABT_ECOLI
BacMap:16130576
General Reference:
  • Bartsch, K., von Johnn-Marteville, A., Schulz, A. (1990). "Molecular analysis of two genes of the Escherichia coli gab cluster: nucleotide sequence of the glutamate:succinic semialdehyde transaminase gene (gabT) and characterization of the succinic semialdehyde dehydrogenase gene (gabD)." J Bacteriol 172:7035-7042. Pubmed: 2254272
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Schulz, A., Taggeselle, P., Tripier, D., Bartsch, K. (1990). "Stereospecific production of the herbicide phosphinothricin (glufosinate) by transamination: isolation and characterization of a phosphinothricin-specific transaminase from Escherichia coli." Appl Environ Microbiol 56:1-6. Pubmed: 2178550
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837