DNA polymerase II (P21189)

Identification
Name:DNA polymerase II
Synonyms:
  • Pol II
Gene Name:polB
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Thought to be involved in DNA repair and/or mutagenesis
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Deoxynucleoside triphosphate+1.0DNA ↔ 1.0Thumb
1.0Deoxynucleoside triphosphate + 1.0DNA ↔ 1.0Pyrophosphate
ReactionCard
EcoCyc Reactions:
1.0DNAn ? 1.0a nucleoside monophosphate
1.0DNAn ? 1.0a nucleoside monophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
binding
catalytic activity
DNA binding
DNA polymerase activity
DNA-directed DNA polymerase activity
nucleic acid binding
nucleotide binding
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular macromolecule metabolic process
cellular nitrogen compound metabolic process
DNA metabolic process
DNA replication
macromolecule metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
Gene Properties
Blattner:b0060
Gene OrientationCounterclockwise
Centisome Percentage:1.37
Left Sequence End63429
Right Sequence End65780
Gene Sequence:
>2352 bp
GTGGCGCAGGCAGGTTTTATCTTAACCCGACACTGGCGGGACACCCCGCAAGGGACAGAA
GTCTCCTTCTGGCTGGCGACGGACAACGGGCCGTTGCAGGTTACGCTTGCACCGCAAGAG
TCCGTGGCGTTTATTCCCGCCGATCAGGTTCCCCGCGCTCAGCATATTTTGCAGGGTGAA
CAAGGCTTTCGCCTGACACCGCTGGCGTTAAAGGATTTTCACCGCCAGCCGGTGTATGGC
CTTTACTGTCGCGCCCATCGCCAATTGATGAATTACGAAAAGCGCCTGCGTGAAGGTGGC
GTTACCGTCTACGAGGCCGATGTGCGTCCGCCAGAACGCTATCTGATGGAGCGGTTTATC
ACCTCACCGGTGTGGGTCGAGGGTGATATGCACAATGGCACTATCGTTAATGCCCGTCTG
AAACCGCATCCCGACTATCGTCCGCCGCTCAAGTGGGTTTCTATAGATATTGAAACCACC
CGCCACGGTGAGCTGTACTGCATCGGCCTGGAAGGCTGCGGGCAGCGCATCGTTTATATG
CTGGGGCCGGAGAATGGCGACGCCTCCTCGCTTGATTTCGAACTGGAATACGTCGCCAGC
CGCCCGCAGTTGCTGGAAAAACTCAACGCCTGGTTTGCCAACTACGATCCTGATGTGATC
ATCGGTTGGAACGTGGTGCAGTTCGATCTGCGAATGCTGCAAAAACATGCCGAGCGTTAC
CGTCTTCCGCTGCGTCTTGGGCGCGATAATAGCGAGCTGGAGTGGCGCGAGCACGGCTTT
AAAAACGGCGTCTTTTTTGCCCAGGCTAAAGGTCGGCTAATTATCGACGGTATCGAGGCG
CTGAAATCCGCGTTCTGGAATTTCTCTTCATTCTCGCTGGAAACTGTCGCTCAGGAGCTA
TTAGGCGAAGGAAAATCTATCGATAACCCGTGGGATCGAATGGACGAAATTGACCGCCGT
TTCGCCGAAGATAAACCTGCGCTGGCAACTTATAACCTGAAAGATTGCGAGCTGGTGACG
CAGATCTTCCACAAAACTGAAATCATGCCATTTTTACTCGAACGGGCAACGGTGAACGGC
CTGCCGGTGGACCGACACGGCGGTTCGGTGGCGGCATTTGGTCATCTCTATTTTCCGCGA
ATGCATCGCGCTGGTTATGTCGCGCCTAATCTCGGCGAAGTGCCGCCGCACGCCAGCCCT
GGCGGCTACGTGATGGATTCACGGCCAGGGCTTTATGATTCAGTGCTGGTGCTGGACTAT
AAAAGCCTGTACCCGTCGATCATCCGCACCTTTCTGATTGATCCCGTCGGGCTGGTGGAA
GGCATGGCGCAGCCTGATCCAGAGCACAGTACCGAAGGTTTTCTCGATGCCTGGTTCTCG
CGAGAAAAACATTGCCTGCCGGAGATTGTGACTAACATCTGGCACGGGCGCGATGAAGCC
AAACGCCAGGGTAACAAACCGCTGTCGCAGGCGCTGAAAATCATCATGAATGCCTTTTAT
GGCGTGCTCGGCACCACCGCCTGCCGCTTCTTCGATCCGCGGCTGGCATCGTCGATCACC
ATGCGTGGTCATCAGATCATGCGGCAAACCAAAGCGTTGATTGAAGCACAGGGCTACGAC
GTTATCTACGGCGATACCGACTCAACGTTTGTCTGGCTGAAAGGCGCACATTCGGAAGAA
GAAGCGGCGAAAATCGGTCGTGCACTGGTGCAGCACGTTAACGCCTGGTGGGCGGAAACG
CTGCAAAAACAACGGCTGACCAGCGCATTAGAACTGGAGTATGAAACCCATTTCTGCCGT
TTTCTGATGCCAACCATTCGCGGAGCCGATACCGGCAGTAAAAAGCGTTATGCCGGACTG
ATTCAGGAGGGCGACAAGCAGCGGATGGTGTTTAAAGGGCTGGAAACCGTGCGCACCGAC
TGGACGCCGCTGGCCCAGCAGTTTCAGCAGGAGCTATACCTGCGCATCTTCCGCAACGAG
CCATATCAGGAATATGTACGCGAAACCATCGACAAACTGATGGCGGGTGAACTGGATGCG
CGACTGGTTTACCGTAAACGCCTTCGCCGTCCGCTGAGCGAGTATCAGCGTAATGTGCCG
CCTCATGTACGCGCCGCTCGCCTTGCCGATGAAGAAAACCAAAAGCGTGGTCGCCCCTTG
CAATATCAGAATCGCGGCACCATTAAGTACGTATGGACCACCAACGGCCCGGAGCCGCTG
GACTACCAACGTTCACCACTGGATTACGAACACTATCTGACCCGCCAGCTACAACCCGTG
GCGGAGGGAATACTCCCTTTTATTGAGGATAATTTTGCTACACTTATGACCGGGCAACTT
GGGCTATTTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:783
Protein Molecular Weight:90052
Protein Theoretical pI:7
PDB File:1Q8I
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>DNA polymerase II
MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAFIPADQVPRAQHILQGE
QGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVTVYEADVRPPERYLMERFI
TSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETTRHGELYCIGLEGCGQRIVYM
LGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPDVIIGWNVVQFDLRMLQKHAERY
RLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIIDGIEALKSAFWNFSSFSLETVAQEL
LGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLKDCELVTQIFHKTEIMPFLLERATVNG
LPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLGEVPPHASPGGYVMDSRPGLYDSVLVLDY
KSLYPSIIRTFLIDPVGLVEGMAQPDPEHSTEGFLDAWFSREKHCLPEIVTNIWHGRDEA
KRQGNKPLSQALKIIMNAFYGVLGTTACRFFDPRLASSITMRGHQIMRQTKALIEAQGYD
VIYGDTDSTFVWLKGAHSEEEAAKIGRALVQHVNAWWAETLQKQRLTSALELEYETHFCR
FLMPTIRGADTGSKKRYAGLIQEGDKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNE
PYQEYVRETIDKLMAGELDARLVYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPL
QYQNRGTIKYVWTTNGPEPLDYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQL
GLF
References
External Links:
ResourceLink
Uniprot ID:P21189
Uniprot Name:DPO2_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674307
PDB ID:1Q8I
Ecogene ID:EG10747
Ecocyc:EG10747
ColiBase:b0060
Kegg Gene:b0060
EchoBASE ID:EB0740
CCDB:DPO2_ECOLI
BacMap:16128054
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bonner, C. A., Hays, S., McEntee, K., Goodman, M. F. (1990). "DNA polymerase II is encoded by the DNA damage-inducible dinA gene of Escherichia coli." Proc Natl Acad Sci U S A 87:7663-7667. Pubmed: 2217198
  • Chen, H., Sun, Y., Stark, T., Beattie, W., Moses, R. E. (1990). "Nucleotide sequence and deletion analysis of the polB gene of Escherichia coli." DNA Cell Biol 9:631-635. Pubmed: 2261080
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Iwasaki, H., Ishino, Y., Toh, H., Nakata, A., Shinagawa, H. (1991). "Escherichia coli DNA polymerase II is homologous to alpha-like DNA polymerases." Mol Gen Genet 226:24-33. Pubmed: 2034216
  • Qiu, Z., Goodman, M. F. (1997). "The Escherichia coli polB locus is identical to dinA, the structural gene for DNA polymerase II. Characterization of Pol II purified from a polB mutant." J Biol Chem 272:8611-8617. Pubmed: 9079692
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901