| Identification |
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| Name: | Primosomal protein N' |
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| Synonyms: | Not Available |
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| Gene Name: | priA |
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| Enzyme Class: | Not Available |
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| Biological Properties |
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| General Function: | response to gamma radiation |
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| Specific Function: | Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA. Is also involved in initiation of normal DNA replication in various plasmids and phages. Binds to branched DNA structures that resemble D-loops or to the primosome assembly site (PAS). Binds to DNA in two distinct modes, either dependent on or independent of the 3' terminus recognition. |
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| Cellular Location: | Not Available |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | |
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| Metabolites: | |
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| GO Classification: | | Function |
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| ATP binding | | ATP-dependent 3'-5' DNA helicase activity | | DNA binding | | DNA recombination | | DNA replication | | DNA replication initiation | | DNA replication, synthesis of RNA primer | | DNA unwinding involved in DNA replication | | DNA-dependent DNA replication | | double-strand break repair | | helicase activity | | plasmid maintenance | | primosome complex | | response to antibiotic | | response to gamma radiation | | zinc ion binding |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >2199
atgcccgttgcccacgttgccttgcccgttccgcttcctcgtacctttgactatctgctg
ccagaaggcatgacggttaaagctgggtgtcgcgtgcgcgtgccgtttggcaaacagcag
gagcgcatcgggattgtggtatcagttagcgatgccagcgaactgccgctcaatgagcta
aaagcggtagtcgaagtgctggatagtgagccggtgtttactcactccgtctggcgattg
ctgctatgggcggcagattactatcatcatccgattggcgatgtgctgtttcatgccttg
ccgattttactacgccaggggcggcctgcggcgaacgcgccgatgtggtactggtttgcc
actgaacaaggccaggcggtggatctgaacagcctgaaacgctcccccaagcaacaacag
gcgctggcggcgttacggcaaggcaaaatctggcgcgaccaggtcgccacgctcgaattt
aatgatgccgcgttgcaggcgctacgcaaaaaaggtctgtgtgatttagcaagtgaaaca
ccagagtttagcgactggcgaacgaactatgccgtttctggtgagcggttgcgattgaat
accgaacaggccaccgccgttggcgcaattcatagcgcggcagatactttttctgcctgg
ctgctggcgggcgttaccggttccggtaaaacggaggtttatctcagcgtactggaaaac
gtgctcgctcagggcaaacaggcgctggtgatggtgccggaaatcggcctgacaccgcaa
actatcgcccgttttcgtgaacgttttaatgcccccgtggaagttctgcattccggcctg
aacgacagcgagcgtctttcggcgtggctgaaagcgaaaaatggtgaggcggcgattgtg
atcggcacccgctccgcgctgtttacgccgtttaaaaatctcggcgtgattgtcattgat
gaagagcacgacagctcctacaagcagcaggaaggctggcgctatcatgcccgcgacctg
gcggtgtatcgtgcgcacagcgagcaaatcccgattattcttggctccgcaacgcccgcg
ctggaaacgttatgcaacgtccagcagaaaaaataccgcctgctgcgcctgacccgtcgg
gcagggaatgcgcgtccggcaattcaacatgtgctggatttaaaaggtcagaaggtgcag
gcaggtctggctccggcgttaatcactcgtatgcgccagcatttacaggctgataaccag
gtcattctctttcttaaccgccgtggctttgcgcctgcactgctgtgccacgactgtggc
tggattgccgaatgcccacgttgcgatcactactacacgctgcatcaggcgcagcaccat
ctgcgctgccaccactgtgacagtcagcgtccggtgccgcgccagtgcccttcctgcggt
tccacgcacctggtccccgtggggctgggcaccgaacagcttgaacagacgctcgcgccg
ttgttccccggcgtgcccatttctcgtatcgaccgcgataccaccagccgcaaaggggcg
ctggaacagcaactggcagaagtacatcgcggcggcgcgcggattttgattggtacacaa
atgctggcgaaaggtcaccatttcccggatgtgacgctggttgcattactggacgtggac
ggcgcgctgttttctgccgattttcgctcggcagagcgtttcgctcagctttacacccag
gtcgccggtcgtgccgggcgtgcgggtaaacagggcgaagtggtgctgcaaacgcaccat
ccggaacatcctctgttgcaaacgttgctctataaaggctacgacgcctttgccgaacag
gcgctggctgagcggcgaatgatgcagctaccgccgtggaccagccatgtgattgtgcgt
gcggaagatcataacaatcagcacgcgccattgttcctgcaacaactgcgtaatctgatc
ctctccagcccactggcagacgagaaactgtgggttctcggtccggttccggctctggca
cctaaacgtggcggtcgctggcgctggcagatattgttgcagcacccttcccgcgtgcgc
ttgcaacacatcattaacggtacgctggcgctcatcaatacaataccggattcccgtaag
gtgaaatgggtgctggatgttgatccgattgagggttaa |
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| Protein Properties |
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| Pfam Domain Function: | Not Available |
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| Protein Residues: | 732 |
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| Protein Molecular Weight: | 81654 |
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| Protein Theoretical pI: | Not Available |
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| PDB File: | 2D7E |
| Signaling Regions: | Not Available |
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| Transmembrane Regions: | Not Available |
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| Protein Sequence: | >Primosomal protein N'
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGIVVSVSDASELPLNEL
KAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFA
TEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEFNDAALQALRKKGLCDLASET
PEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIV
IGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPA
LETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQAGLAPALITRMRQHLQADNQ
VILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQ
MLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHH
PEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLI
LSSPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG |
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| References |
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| External Links: | |
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| General Reference: | Not Available |
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