Identification
Name:tRNA dimethylallyltransferase
Synonyms:
  • Dimethylallyl diphosphate:tRNA dimethylallyltransferase
  • DMAPP:tRNA dimethylallyltransferase
  • DMATase
  • Isopentenyl-diphosphate:tRNA isopentenyltransferase
  • IPP transferase
  • IPPT
  • IPTase
Gene Name:miaA
Enzyme Class:
Biological Properties
General Function:Involved in ATP binding
Specific Function:Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0tRNA1.0Thumb+1.0tRNA containing 6-isopentenyladenosine
1.0Dimethylallylpyrophosphate + 1.0tRNA ↔ 1.0Pyrophosphate + 1.0tRNA containing 6-isopentenyladenosine
ReactionCard
Complex Reactions:
1.0Thumb+1.0tRNA1.0Thumb+1.0tRNA containing 6-dimethylallyladenosine
1.0Dimethylallylpyrophosphate + 1.0tRNA → 1.0Pyrophosphate + 1.0tRNA containing 6-dimethylallyladenosine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01120DimethylallylpyrophosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
nucleoside binding
purine nucleoside binding
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
tRNA metabolic process
tRNA processing
Gene Properties
Blattner:b4171
Gene OrientationClockwise
Centisome Percentage:94.78
Left Sequence End4397275
Right Sequence End4398225
Gene Sequence:
>951 bp
ATGCAGATCCTGTTGGCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGC
ATTGTTGAAAACGCGCTGGCCATTTACGGCGCACCGATATATGTCCGTCACGAAGTGGTA
CATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATT
AGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTA
CGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACC
AAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGT
CACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGA
ATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAG
CTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCG
CTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACG
ACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTC
GGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAA
CGCGCGTTTTTGATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAA
TGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAGAATGTGGTGGCA
CGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATT
GTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:316
Protein Molecular Weight:35065
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>tRNA dimethylallyltransferase
MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSALIYKGMDIGTAKPNAE
ELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIPLLVGGTMLYFKALLEGLS
PLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARIHPNDPQRLSRALEVFFISGK
TLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRFHQMLASGFEAEVRALFARGDLH
TDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATRQLAKRQITWLRGWEGVHWLDSEKP
EQARDEVLQVVGAIAG
References
External Links:
ResourceLink
Uniprot ID:P16384
Uniprot Name:MIAA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321911
Ecogene ID:EG10595
Ecocyc:EG10595
ColiBase:b4171
Kegg Gene:b4171
EchoBASE ID:EB0590
CCDB:MIAA_ECOLI
BacMap:16131993
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Connolly, D. M., Winkler, M. E. (1989). "Genetic and physiological relationships among the miaA gene, 2-methylthio-N6-(delta 2-isopentenyl)-adenosine tRNA modification, and spontaneous mutagenesis in Escherichia coli K-12." J Bacteriol 171:3233-3246. Pubmed: 2656644
  • Connolly, D. M., Winkler, M. E. (1991). "Structure of Escherichia coli K-12 miaA and characterization of the mutator phenotype caused by miaA insertion mutations." J Bacteriol 173:1711-1721. Pubmed: 1999389
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kajitani, M., Ishihama, A. (1991). "Identification and sequence determination of the host factor gene for bacteriophage Q beta." Nucleic Acids Res 19:1063-1066. Pubmed: 2020545
  • Leung, H. C., Chen, Y., Winkler, M. E. (1997). "Regulation of substrate recognition by the MiaA tRNA prenyltransferase modification enzyme of Escherichia coli K-12." J Biol Chem 272:13073-13083. Pubmed: 9148919
  • Moore, J. A., Poulter, C. D. (1997). "Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: a binding mechanism for recombinant enzyme." Biochemistry 36:604-614. Pubmed: 9012675
  • Seif, E., Hallberg, B. M. (2009). "RNA-protein mutually induced fit: structure of Escherichia coli isopentenyl-tRNA transferase in complex with tRNA(Phe)." J Biol Chem 284:6600-6604. Pubmed: 19158097
  • Tsui, H. T., Mandavilli, B. S., Winkler, M. E. (1992). "Nonconserved segment of the MutL protein from Escherichia coli K-12 and Salmonella typhimurium." Nucleic Acids Res 20:2379. Pubmed: 1594459