Identification
Name:Xaa-Pro aminopeptidase
Synonyms:
  • Aminoacylproline aminopeptidase
  • Aminopeptidase P II
  • APP-II
  • X-Pro aminopeptidase
Gene Name:pepP
Enzyme Class:
Biological Properties
General Function:Involved in aminopeptidase activity
Specific Function:Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
GO Classification:
Function
aminopeptidase activity
binding
catalytic activity
cation binding
exopeptidase activity
hydrolase activity
ion binding
manganese ion binding
metal ion binding
metalloexopeptidase activity
metallopeptidase activity
peptidase activity
peptidase activity, acting on L-amino acid peptides
transition metal ion binding
Process
cellular process
macromolecule metabolic process
metabolic process
protein metabolic process
proteolysis
Gene Properties
Blattner:b2908
Gene OrientationCounterclockwise
Centisome Percentage:65.77
Left Sequence End3051537
Right Sequence End3052862
Gene Sequence:
>1326 bp
ATGAGTGAGATATCCCGGCAAGAGTTTCAGCGTCGCCGTCAGGCCCTGGTGGAGCAAATG
CAACCCGGCAGCGCCGCGCTGATTTTTGCTGCACCAGAAGTAACACGTAGCGCCGACAGC
GAATACCCCTATCGTCAGAACAGTGACTTCTGGTACTTCACCGGCTTTAACGAACCGGAA
GCGGTGCTGGTGCTGATTAAAAGCGATGACACTCATAACCACAGCGTTCTGTTTAACCGC
GTTCGCGACCTGACGGCGGAGATCTGGTTTGGCCGTCGCTTAGGCCAGGATGCCGCGCCA
GAGAAACTGGGCGTTGACCGCGCACTGGCATTCAGCGAAATCAATCAGCAACTTTATCAA
CTACTTAACGGCCTGGATGTGGTTTACCATGCCCAGGGCGAATATGCATATGCTGATGTA
ATCGTGAACAGTGCGCTGGAAAAACTGCGTAAAGGTTCGCGGCAAAATCTCACCGCACCG
GCAACGATGATCGACTGGCGTCCTGTTGTTCATGAAATGCGCCTGTTCAAATCGCCAGAA
GAGATTGCCGTACTCCGCCGCGCGGGAGAAATCACCGCCATGGCACATACACGGGCGATG
GAAAAATGCCGTCCGGGAATGTTCGAGTACCATCTGGAAGGCGAAATTCACCACGAATTT
AACCGCCACGGTGCGCGCTATCCGTCCTATAACACCATTGTCGGCAGCGGTGAAAACGGC
TGCATTCTGCACTACACCGAAAACGAGTGTGAAATGCGCGACGGCGACCTGGTGTTGATT
GACGCGGGTTGTGAATACAAAGGTTACGCTGGCGATATTACCCGCACCTTCCCGGTCAAC
GGCAAATTCACCCAGGCCCAGCGTGAAATCTACGACATTGTGCTGGAGTCTCTCGAAACC
AGCCTGCGCCTGTATCGTCCGGGAACTTCCATTCTGGAAGTCACTGGTGAAGTGGTGCGC
ATCATGGTTAGCGGCCTGGTAAAACTCGGCATCCTGAAAGGTGATGTTGATGAACTGATC
GCTCAGAACGCCCATCGTCCTTTCTTTATGCATGGCCTTAGCCACTGGTTAGGACTGGAT
GTCCATGACGTGGGTGTTTATGGTCAGGATCGCTCGCGCATTCTGGAACCGGGCATGGTA
CTGACCGTAGAGCCAGGGCTGTATATTGCGCCGGATGCAGAAGTGCCAGAACAATATCGC
GGTATCGGCATTCGTATTGAAGACGACATTGTGATTACCGAAACCGGTAACGAAAACCTC
ACCGCCAGCGTGGTGAAAAAGCCGGAAGAAATCGAAGCGTTGATGGTTGCTGCGAGAAAG
CAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:441
Protein Molecular Weight:49815
Protein Theoretical pI:5
PDB File:1M35
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Xaa-Pro aminopeptidase
MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPYRQNSDFWYFTGFNEPE
AVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEKLGVDRALAFSEINQQLYQ
LLNGLDVVYHAQGEYAYADVIVNSALEKLRKGSRQNLTAPATMIDWRPVVHEMRLFKSPE
EIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHHEFNRHGARYPSYNTIVGSGENG
CILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRTFPVNGKFTQAQREIYDIVLESLET
SLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKGDVDELIAQNAHRPFFMHGLSHWLGLD
VHDVGVYGQDRSRILEPGMVLTVEPGLYIAPDAEVPEQYRGIGIRIEDDIVITETGNENL
TASVVKKPEEIEALMVAARKQ
References
External Links:
ResourceLink
Uniprot ID:P15034
Uniprot Name:AMPP_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675720
PDB ID:1M35
Ecogene ID:EG10697
Ecocyc:EG10697
ColiBase:b2908
Kegg Gene:b2908
EchoBASE ID:EB0691
CCDB:AMPP_ECOLI
BacMap:16130810
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Nakahigashi, K., Miyamoto, K., Nishimura, K., Inokuchi, H. (1992). "Isolation and characterization of a light-sensitive mutant of Escherichia coli K-12 with a mutation in a gene that is required for the biosynthesis of ubiquinone." J Bacteriol 174:7352-7359. Pubmed: 1339425
  • Wilce, M. C., Bond, C. S., Dixon, N. E., Freeman, H. C., Guss, J. M., Lilley, P. E., Wilce, J. A. (1998). "Structure and mechanism of a proline-specific aminopeptidase from Escherichia coli." Proc Natl Acad Sci U S A 95:3472-3477. Pubmed: 9520390
  • Yoshimoto, T., Tone, H., Honda, T., Osatomi, K., Kobayashi, R., Tsuru, D. (1989). "Sequencing and high expression of aminopeptidase P gene from Escherichia coli HB101." J Biochem 105:412-416. Pubmed: 2659585