Identification |
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Name: | DNA topoisomerase 3 |
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Synonyms: | Not Available |
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Gene Name: | topB |
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Enzyme Class: | |
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Biological Properties |
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General Function: | DNA topological change |
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Specific Function: | Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. TOP3 is a potent decatenase. |
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Cellular Location: | Not Available |
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SMPDB Pathways: | Not Available |
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KEGG Pathways: | Not Available |
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Metabolites: | |
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GO Classification: | Function |
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chromosome separation | DNA binding | DNA recombination | DNA topoisomerase type I activity | DNA topological change | magnesium ion binding |
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Gene Properties |
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Blattner: | Not Available |
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Gene Orientation | Not Available |
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Centisome Percentage: | Not Available |
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Left Sequence End | Not Available |
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Right Sequence End | Not Available |
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Gene Sequence: | >1962
atgcggttgtttattgccgaaaaaccgagtctggcgcgcgccattgctgatgtcctgccc
aaaccgcaccggaaaggcgatggctttatcgagtgcggtaatggtcaggtggtgacctgg
tgtatcggtcacctgcttgagcaggcgcagccagacgcctacgacagccgctatgcgcgc
tggaatcttgcggatttgccgattgtcccggaaaagtggcaattacagccccgaccctcc
gtgaccaaacaacttaacgtcatcaaacggttcctgcatgaagccagcgaaatcgttcac
gccggggacccggatcgtgaagggcaattgctggtggatgaagtgctggactatctgcaa
ctggcaccggaaaagcgccagcaggtacagcgttgcttgataaacgacctgaacccgcag
gcggttgagcgggcgatcgaccgtcttcgttccaacagtgagtttgtaccgctgtgcgtt
tctgcgctggcgcgagcgcgtgccgactggctgtacggcatcaatatgacccgtgcgtat
accattctcggtcgcaatgccggttatcagggcgtactttccgtgggacgcgtgcagacg
cccgtgcttgggctggtggtgcgccgcgatgaagagattgaaaacttcgtggcgaaagac
ttctttgaagtcaaagcacatatcgtgacacctgccgatgagcggtttaccgctatctgg
caaccgagcgaagcgtgtgaaccgtaccaggatgaagaagggcgcttgttacatcgtcca
ctggcggagcatgtggttaaccgcattagtggtcaaccggctattgtcaccagctataac
gataaacgggaatcagaatccgcgccgctgcctttttcgctttcagcgttgcagattgaa
gcggcaaaacgttttggtctgagtgcgcagaacgtgcttgatatctgccagaaactgtac
gaaacgcacaagctaatcacttatccgcgttctgattgtcgctatttgccagaagaacat
tttgccggacgccacgcggtgatgaatgccatcagtgttcatgcaccggatctgttgccg
cagccagtggtagatccagatatacgcaaccgctgttgggatgacaaaaaggtcgatgcg
caccacgccatcattccgaccgcacggagttctgcgatcaacctgacggagaacgaagcg
aaggtctataacctgattgcccgtcagtatctgatgcaattctgcccggatgcggtgttc
cgcaagtgtgttatcgaactggacattgccaaaggcaaatttgtcgctaaagcgcgtttt
cttgctgaagcaggctggcgcacgctgttaggcagcaaagagcgcgatgaagaaaacgac
ggcacgccactgcctgtggtggcgaaaggcgatgagttgctgtgtgaaaaaggtgaagtg
gtagagcggcaaacccagccgccgcgccattttaccgatgcaacactgctttcggcgatg
accgggatcgcgcgctttgtgcaggataaagatctgaaaaagatcctccgtgcgaccgat
ggtctggggacagaggcaacgcgtgccgggattattgaactgttgttcaagcgtggtttc
ctgaccaaaaaagggcgctatatccactccaccgacgccggaaaagcgctattccattcg
ctgccggagatggcgacgcgaccggacatgaccgcgcactgggaatcggtgctgacgcaa
atcagcgaaaagcagtgtcgctatcaggactttatgcagccgctggtggggacgctatat
cagcttattgatcaagccaaacgtacgccggtgcggcagtttcgcggcattgtggctccg
ggcagtggtggcagtgctgataagaaaaaggctgcaccgcgtaaacgtagtgcgaaaaaa
agtccgccagcagatgaagtcggaagcggggcgatagcgtaa |
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Protein Properties |
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Pfam Domain Function: | Not Available |
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Protein Residues: | 653 |
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Protein Molecular Weight: | 73216 |
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Protein Theoretical pI: | Not Available |
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PDB File: | 1D6M |
Signaling Regions: | Not Available |
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Transmembrane Regions: | Not Available |
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Protein Sequence: | >DNA topoisomerase 3
MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGHLLEQAQPDAYDSRYAR
WNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAGDPDREGQLLVDEVLDYLQ
LAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCVSALARARADWLYGINMTRAY
TILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVAKDFFEVKAHIVTPADERFTAIW
QPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIVTSYNDKRESESAPLPFSLSALQIE
AAKRFGLSAQNVLDICQKLYETHKLITYPRSDCRYLPEEHFAGRHAVMNAISVHAPDLLP
QPVVDPDIRNRCWDDKKVDAHHAIIPTARSSAINLTENEAKVYNLIARQYLMQFCPDAVF
RKCVIELDIAKGKFVAKARFLAEAGWRTLLGSKERDEENDGTPLPVVAKGDELLCEKGEV
VERQTQPPRHFTDATLLSAMTGIARFVQDKDLKKILRATDGLGTEATRAGIIELLFKRGF
LTKKGRYIHSTDAGKALFHSLPEMATRPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLY
QLIDQAKRTPVRQFRGIVAPGSGGSADKKKAAPRKRSAKKSPPADEVGSGAIA |
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References |
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External Links: | |
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General Reference: | Not Available |
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