Identification
Name:DNA topoisomerase 3
Synonyms:Not Available
Gene Name:topB
Enzyme Class:
Biological Properties
General Function:DNA topological change
Specific Function:Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. TOP3 is a potent decatenase.
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
GO Classification:
Function
chromosome separation
DNA binding
DNA recombination
DNA topoisomerase type I activity
DNA topological change
magnesium ion binding
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1962
atgcggttgtttattgccgaaaaaccgagtctggcgcgcgccattgctgatgtcctgccc
aaaccgcaccggaaaggcgatggctttatcgagtgcggtaatggtcaggtggtgacctgg
tgtatcggtcacctgcttgagcaggcgcagccagacgcctacgacagccgctatgcgcgc
tggaatcttgcggatttgccgattgtcccggaaaagtggcaattacagccccgaccctcc
gtgaccaaacaacttaacgtcatcaaacggttcctgcatgaagccagcgaaatcgttcac
gccggggacccggatcgtgaagggcaattgctggtggatgaagtgctggactatctgcaa
ctggcaccggaaaagcgccagcaggtacagcgttgcttgataaacgacctgaacccgcag
gcggttgagcgggcgatcgaccgtcttcgttccaacagtgagtttgtaccgctgtgcgtt
tctgcgctggcgcgagcgcgtgccgactggctgtacggcatcaatatgacccgtgcgtat
accattctcggtcgcaatgccggttatcagggcgtactttccgtgggacgcgtgcagacg
cccgtgcttgggctggtggtgcgccgcgatgaagagattgaaaacttcgtggcgaaagac
ttctttgaagtcaaagcacatatcgtgacacctgccgatgagcggtttaccgctatctgg
caaccgagcgaagcgtgtgaaccgtaccaggatgaagaagggcgcttgttacatcgtcca
ctggcggagcatgtggttaaccgcattagtggtcaaccggctattgtcaccagctataac
gataaacgggaatcagaatccgcgccgctgcctttttcgctttcagcgttgcagattgaa
gcggcaaaacgttttggtctgagtgcgcagaacgtgcttgatatctgccagaaactgtac
gaaacgcacaagctaatcacttatccgcgttctgattgtcgctatttgccagaagaacat
tttgccggacgccacgcggtgatgaatgccatcagtgttcatgcaccggatctgttgccg
cagccagtggtagatccagatatacgcaaccgctgttgggatgacaaaaaggtcgatgcg
caccacgccatcattccgaccgcacggagttctgcgatcaacctgacggagaacgaagcg
aaggtctataacctgattgcccgtcagtatctgatgcaattctgcccggatgcggtgttc
cgcaagtgtgttatcgaactggacattgccaaaggcaaatttgtcgctaaagcgcgtttt
cttgctgaagcaggctggcgcacgctgttaggcagcaaagagcgcgatgaagaaaacgac
ggcacgccactgcctgtggtggcgaaaggcgatgagttgctgtgtgaaaaaggtgaagtg
gtagagcggcaaacccagccgccgcgccattttaccgatgcaacactgctttcggcgatg
accgggatcgcgcgctttgtgcaggataaagatctgaaaaagatcctccgtgcgaccgat
ggtctggggacagaggcaacgcgtgccgggattattgaactgttgttcaagcgtggtttc
ctgaccaaaaaagggcgctatatccactccaccgacgccggaaaagcgctattccattcg
ctgccggagatggcgacgcgaccggacatgaccgcgcactgggaatcggtgctgacgcaa
atcagcgaaaagcagtgtcgctatcaggactttatgcagccgctggtggggacgctatat
cagcttattgatcaagccaaacgtacgccggtgcggcagtttcgcggcattgtggctccg
ggcagtggtggcagtgctgataagaaaaaggctgcaccgcgtaaacgtagtgcgaaaaaa
agtccgccagcagatgaagtcggaagcggggcgatagcgtaa
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:653
Protein Molecular Weight:73216
Protein Theoretical pI:Not Available
PDB File:1D6M
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>DNA topoisomerase 3
MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGHLLEQAQPDAYDSRYAR
WNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAGDPDREGQLLVDEVLDYLQ
LAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCVSALARARADWLYGINMTRAY
TILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVAKDFFEVKAHIVTPADERFTAIW
QPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIVTSYNDKRESESAPLPFSLSALQIE
AAKRFGLSAQNVLDICQKLYETHKLITYPRSDCRYLPEEHFAGRHAVMNAISVHAPDLLP
QPVVDPDIRNRCWDDKKVDAHHAIIPTARSSAINLTENEAKVYNLIARQYLMQFCPDAVF
RKCVIELDIAKGKFVAKARFLAEAGWRTLLGSKERDEENDGTPLPVVAKGDELLCEKGEV
VERQTQPPRHFTDATLLSAMTGIARFVQDKDLKKILRATDGLGTEATRAGIIELLFKRGF
LTKKGRYIHSTDAGKALFHSLPEMATRPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLY
QLIDQAKRTPVRQFRGIVAPGSGGSADKKKAAPRKRSAKKSPPADEVGSGAIA
References
External Links:
ResourceLink
Uniprot ID:P14294
Uniprot Name:TOP3_ECOLI
PDB ID:1D6M
CCDB:TOP3_ECOLI
General Reference:Not Available