Inactive ribonuclease PH (P0CG19)

Identification
Name:Inactive ribonuclease PH
Synonyms:
  • RNase PH
  • tRNA nucleotidyltransferase
Gene Name:rph
Enzyme Class:
Biological Properties
General Function:Cell cycle control, cell division, chromosome partitioning
Specific Function:Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates. Also acts to regulate the attenuation of pyrE
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
1.0tRNA(n+1)+1.0Thumb → 1.0tRNA(n)+1.0a nucleoside diphosphate
1.0tRNA(n+1) + 1.0Inorganic phosphate → 1.0tRNA(n) + 1.0a nucleoside diphosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21380Inorganic phosphateMetaboCard
GO Classification:
Function
3'-5' exonuclease activity
3'-5'-exoribonuclease activity
binding
catalytic activity
exonuclease activity
hydrolase activity
hydrolase activity, acting on ester bonds
nuclease activity
nucleic acid binding
ribonuclease activity
RNA binding
tRNA binding
tRNA-specific ribonuclease activity
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
RNA processing
tRNA metabolic process
tRNA processing
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>687 bp
ATGCGTCCAGCAGGCCGTAGCAATAATCAGGTGCGTCCCGTTACCCTGACTCGTAACTAT
ACAAAACATGCAGAAGGCTCGGTGCTGGTCGAATTTGGCGATACCAAAGTGTTGTGTACC
GCCTCTATTGAAGAAGGCGTGCCGCGCTTCCTGAAAGGTCAGGGCCAGGGCTGGATCACC
GCAGAGTACGGCATGCTGCCACGTTCTACCCACACCCGTAACGCTCGTGAAGCGGCGAAA
GGTAAGCAGGGTGGACGCACAATGGAAATCCAGCGTCTGATCGCCCGTGCTCTTCGCGCG
GCAGTAGATTTGAAAGCGCTGGGTGAGTTCACCATTACGCTGGACTGCGACGTGCTTCAG
GCTGATGGTGGCACGCGTACCGCGTCGATTACGGGTGCCTGCGTGGCGCTGGTAGATGCG
CTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCC
GCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGAC
TCTGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTG
CAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTG
GCCCGAGGGGAATCGAATCCATTGTAG
Protein Properties
Pfam Domain Function:
Protein Residues:228
Protein Molecular Weight:24425
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Inactive ribonuclease PH
MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWIT
AEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQ
ADGGTRTASITGACVALVDALQKLVENGKLKTNPMKGMVAAVSVGIVNGEAVCDLEYVED
SAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLILLALARGESNPL
References
External Links:
ResourceLink
Uniprot ID:P0CG19
Uniprot Name:RNPH_ECOLI
GenBank Gene ID:L10328
Genebank Protein ID:290493
CCDB:RNPH_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jensen, K. F. (1993). "The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels." J Bacteriol 175:3401-3407. Pubmed: 8501045