ECMDB: Inactive ribonuclease PH (P0CG19)

Identification

Name:

Inactive ribonuclease PH

Synonyms:

RNase PH
tRNA nucleotidyltransferase

Gene Name:

rph

Enzyme Class:

2.7.7.56

Biological Properties

General Function:

Cell cycle control, cell division, chromosome partitioning

Specific Function:

Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates. Also acts to regulate the attenuation of pyrE

Cellular Location:

Cytoplasmic

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

Complex Reactions:

1.0tRNA(n+1)

1.0

→

1.0tRNA(n)

1.0a nucleoside diphosphate

1.0tRNA(n+1) + 1.0Inorganic phosphate → 1.0tRNA(n) + 1.0a nucleoside diphosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21380	Inorganic phosphate	MetaboCard

GO Classification:

Function
3'-5' exonuclease activity
3'-5'-exoribonuclease activity
binding
catalytic activity
exonuclease activity
hydrolase activity
hydrolase activity, acting on ester bonds
nuclease activity
nucleic acid binding
ribonuclease activity
RNA binding
tRNA binding
tRNA-specific ribonuclease activity
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
RNA processing
tRNA metabolic process
tRNA processing

Gene Properties

Blattner:

Not Available

Gene Orientation

Not Available

Centisome Percentage:

Not Available

Left Sequence End

Not Available

Right Sequence End

Not Available

Gene Sequence:

>687 bp
ATGCGTCCAGCAGGCCGTAGCAATAATCAGGTGCGTCCCGTTACCCTGACTCGTAACTAT
ACAAAACATGCAGAAGGCTCGGTGCTGGTCGAATTTGGCGATACCAAAGTGTTGTGTACC
GCCTCTATTGAAGAAGGCGTGCCGCGCTTCCTGAAAGGTCAGGGCCAGGGCTGGATCACC
GCAGAGTACGGCATGCTGCCACGTTCTACCCACACCCGTAACGCTCGTGAAGCGGCGAAA
GGTAAGCAGGGTGGACGCACAATGGAAATCCAGCGTCTGATCGCCCGTGCTCTTCGCGCG
GCAGTAGATTTGAAAGCGCTGGGTGAGTTCACCATTACGCTGGACTGCGACGTGCTTCAG
GCTGATGGTGGCACGCGTACCGCGTCGATTACGGGTGCCTGCGTGGCGCTGGTAGATGCG
CTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCC
GCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGAC
TCTGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTG
CAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTG
GCCCGAGGGGAATCGAATCCATTGTAG

Protein Properties

Pfam Domain Function:

RNase_PH (PF01138 )
RNase_PH_C (PF03725 )

Protein Residues:

228

Protein Molecular Weight:

24425

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Inactive ribonuclease PH
MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWIT
AEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQ
ADGGTRTASITGACVALVDALQKLVENGKLKTNPMKGMVAAVSVGIVNGEAVCDLEYVED
SAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLILLALARGESNPL

References

External Links:

Resource	Link
Uniprot ID:	P0CG19
Uniprot Name:	RNPH_ECOLI
GenBank Gene ID:	L10328
Genebank Protein ID:	290493
CCDB:	RNPH_ECOLI

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Jensen, K. F. (1993). "The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels." J Bacteriol 175:3401-3407. Pubmed: 8501045