Endo-type membrane-bound lytic murein transglycosylase A (P0C960)

Identification
Name:Endo-type membrane-bound lytic murein transglycosylase A
Synonyms:
  • Peptidoglycan lytic endotransglycosylase
Gene Name:emtA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in lytic endotransglycosylase activity
Specific Function:Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Preferentially cleaves at a distance of more than two disaccharide units from the ends of the glycan chain. Prefers cross-linked murein in vivo
Cellular Location:Cell outer membrane; Lipid-anchor (Probable)
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
EcoCyc Reactions:
1.0a peptidoglycan polymer ? 1.0a peptidoglycan polymer with 1,6-anhydromuropeptide end+1.0a peptidoglycan polymer with GlcNAc end
1.0a peptidoglycan polymer ? 1.0a peptidoglycan polymer with 1,6-anhydromuropeptide end + 1.0a peptidoglycan polymer with GlcNAc end
ReactionCard
Complex Reactions:
1.0two linked disacharide tetrapeptide and tripeptide murein units (uncrosslinked, middle of chain) → 1.0Thumb+1.0Thumb
1.0two linked disacharide tetrapeptide and tripeptide murein units (uncrosslinked, middle of chain) → 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide + 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide
ReactionCard
1.0two linked disacharide tripeptide murein units (uncrosslinked, middle of chain) → 2.0Thumb
1.0two linked disacharide tripeptide murein units (uncrosslinked, middle of chain) → 2.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide
ReactionCard
1.0two linked disacharide tetrapeptide murein units (uncrosslinked, middle of chain) → 2.0Thumb
1.0two linked disacharide tetrapeptide murein units (uncrosslinked, middle of chain) → 2.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide
ReactionCard
1.0three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala), one uncrosslinked tetrapaptide) (middle of chain) → 1.0Thumb+1.0two disacharide linked murein units, tetrapeptide corsslinked tetrapeptide (A2pm->D-ala) (middle of chain)
1.0three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala), one uncrosslinked tetrapaptide) (middle of chain) → 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide + 1.0two disacharide linked murein units, tetrapeptide corsslinked tetrapeptide (A2pm->D-ala) (middle of chain)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21249N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptideMetaboCard
ECMDB21250N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptideMetaboCard
GO Classification:
Component
cell part
membrane
Function
catalytic activity
lytic transglycosylase activity
transferase activity
transferase activity, transferring glycosyl groups
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b1193
Gene OrientationClockwise
Centisome Percentage:26.78
Left Sequence End1242403
Right Sequence End1243014
Gene Sequence:
>612 bp
GTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCAT
GACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATG
CCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATT
ATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGTAAATCGAATGCCATTGGTTTG
ATGCAGTTAAAAGCTTCAACCTCCGGACGTGATGTTTATCGCCGTATGGGCTGGAGTGGT
GAGCCGACGACCAGCGAGCTGAAAAATCCAGAGCGTAATATTTCAATGGGGGCGGCTTAC
CTGAATATTCTGGAAACCGGCCCGCTGGCAGGCATTGAAGATCCGAAGGTACTGCAATAT
GCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTACGGACTTTCTCGTCAGAT
CGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTA
GCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTG
GACGCGATGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:203
Protein Molecular Weight:22226
Protein Theoretical pI:10
Signaling Regions:
  • 1-15
Transmembrane Regions:
  • None
Protein Sequence:
>Endo-type membrane-bound lytic murein transglycosylase A
MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQKAGAAWGVDPQLITAI
IAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEPTTSELKNPERNISMGAAY
LNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSDRKKAISKINDLDADEFLEHV
ARNHPAPQAPRYIYKLEQALDAM
References
External Links:
ResourceLink
Uniprot ID:P0C960
Uniprot Name:EMTA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674869
Ecogene ID:EG13897
Ecocyc:EG13897
ColiBase:b1193
Kegg Gene:b1193
EchoBASE ID:EB3656
CCDB:EMTA_ECOLI
BacMap:90111231
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kraft, A. R., Templin, M. F., Holtje, J. V. (1998). "Membrane-bound lytic endotransglycosylase in Escherichia coli." J Bacteriol 180:3441-3447. Pubmed: 9642199
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232