Penicillin-insensitive murein endopeptidase (P0C0T5)

Identification
Name:Penicillin-insensitive murein endopeptidase
Synonyms:
  • D-alanyl-D-alanine-endopeptidase
  • DD-endopeptidase
Gene Name:mepA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in serine-type endopeptidase activity
Specific Function:Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein
Cellular Location:Periplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
1.0Thumb+1.0three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala) & tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain) → 1.0three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala), one uncrosslinked tetrapaptide) (middle of chain)
1.0Water + 1.0three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala) & tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain) → 1.0three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala), one uncrosslinked tetrapaptide) (middle of chain)
ReactionCard
1.0Thumb+1.0two disacharide linked murein units, pentapeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain) → 1.0two linked disacharide pentapeptide and tetrapeptide murein units (uncrosslinked, middle of chain)
1.0Water + 1.0two disacharide linked murein units, pentapeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain) → 1.0two linked disacharide pentapeptide and tetrapeptide murein units (uncrosslinked, middle of chain)
ReactionCard
1.0Thumb+1.0two disacharide linked murein units, tetrapeptide corsslinked tetrapeptide (A2pm->D-ala) (middle of chain) → 1.0two linked disacharide tetrapeptide murein units (uncrosslinked, middle of chain)
1.0Water + 1.0two disacharide linked murein units, tetrapeptide corsslinked tetrapeptide (A2pm->D-ala) (middle of chain) → 1.0two linked disacharide tetrapeptide murein units (uncrosslinked, middle of chain)
ReactionCard
1.0Thumb+1.0two disacharide linked murein units, tripeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain) → 1.0two linked disacharide tetrapeptide and tripeptide murein units (uncrosslinked, middle of chain)
1.0Water + 1.0two disacharide linked murein units, tripeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain) → 1.0two linked disacharide tetrapeptide and tripeptide murein units (uncrosslinked, middle of chain)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
outer membrane-bounded periplasmic space
periplasmic space
Function
catalytic activity
endopeptidase activity
hydrolase activity
peptidase activity
peptidase activity, acting on L-amino acid peptides
serine-type endopeptidase activity
Process
macromolecule metabolic process
metabolic process
protein metabolic process
proteolysis
Gene Properties
Blattner:b2328
Gene OrientationCounterclockwise
Centisome Percentage:52.67
Left Sequence End2443582
Right Sequence End2444406
Gene Sequence:
>825 bp
GTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCG
CAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAA
CATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG
TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCAT
TTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTG
CCGGATACCAACGAACTGGCAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGC
TGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGG
CGGACAGTGGGTTGGCTGCGAATGTTAAAACGTCTGCGCAAAGCGCGGGAACCACGGCCT
TTACGCTTAAGTCCATTGCATATGGATGTCCACGCCGGAAATTTAGTGCATAGCGCGTCA
GGGTTAAAACTCATCGACTGGGAGTATGCCGGAGATGGTGATATCGCGCTGGAACTGGCG
GCGGTGTGGGTGGAAAATACTGAACAGCACCGGCAATTGGTCAATGACTATGCCACTCGC
GCGAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTG
ATGCTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATC
AGGCTGGCCGATGACACCTGGCGGCAGCTATTAATAAAACAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:274
Protein Molecular Weight:30136
Protein Theoretical pI:8
PDB File:1TZP
Signaling Regions:
  • 1-19
Transmembrane Regions:
  • None
Protein Sequence:
>Penicillin-insensitive murein endopeptidase
MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGCIVGADTLPIQSEHYQV
MRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPAGGRFNGGHASHQTGLDVD
IFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEIFSLIKLAAQDKDVTRIFVNP
AIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPADSLECEDQPLPPSGDGCGAELQ
SWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI
References
External Links:
ResourceLink
Uniprot ID:P0C0T5
Uniprot Name:MEPA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062670
PDB ID:1TZP
Ecogene ID:EG10580
Ecocyc:EG10580
ColiBase:b2328
Kegg Gene:b2328
EchoBASE ID:EB0575
CCDB:MEPA_ECOLI
BacMap:16130263
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Keck, W., van Leeuwen, A. M., Huber, M., Goodell, E. W. (1990). "Cloning and characterization of mepA, the structural gene of the penicillin-insensitive murein endopeptidase from Escherichia coli." Mol Microbiol 4:209-219. Pubmed: 2187143
  • Marcyjaniak, M., Odintsov, S. G., Sabala, I., Bochtler, M. (2004). "Peptidoglycan amidase MepA is a LAS metallopeptidase." J Biol Chem 279:43982-43989. Pubmed: 15292190
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837