Membrane-bound lytic murein transglycosylase C (P0C066)

Identification
Name:Membrane-bound lytic murein transglycosylase C
Synonyms:
  • Murein hydrolase C
Gene Name:mltC
Enzyme Class:Not Available
Biological Properties
General Function:Involved in hydrolase activity, acting on glycosyl bonds
Specific Function:Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division
Cellular Location:Cell outer membrane; Lipid-anchor (Probable)
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
EcoCyc Reactions:
1.0a peptidoglycan polymer ? 1.0a peptidoglycan polymer with 1,6-anhydromuropeptide end+1.0a peptidoglycan polymer with GlcNAc end
1.0a peptidoglycan polymer ? 1.0a peptidoglycan polymer with 1,6-anhydromuropeptide end + 1.0a peptidoglycan polymer with GlcNAc end
ReactionCard
Complex Reactions:
1.0two linked disacharide tetrapeptide and tripeptide murein units (uncrosslinked, middle of chain) → 1.0Thumb+1.0Thumb
1.0two linked disacharide tetrapeptide and tripeptide murein units (uncrosslinked, middle of chain) → 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide + 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide
ReactionCard
1.0two linked disacharide tripeptide murein units (uncrosslinked, middle of chain) → 2.0Thumb
1.0two linked disacharide tripeptide murein units (uncrosslinked, middle of chain) → 2.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide
ReactionCard
1.0two linked disacharide tetrapeptide murein units (uncrosslinked, middle of chain) → 2.0Thumb
1.0two linked disacharide tetrapeptide murein units (uncrosslinked, middle of chain) → 2.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide
ReactionCard
1.0three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala), one uncrosslinked tetrapaptide) (middle of chain) → 1.0Thumb+1.0two disacharide linked murein units, tetrapeptide corsslinked tetrapeptide (A2pm->D-ala) (middle of chain)
1.0three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala), one uncrosslinked tetrapaptide) (middle of chain) → 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide + 1.0two disacharide linked murein units, tetrapeptide corsslinked tetrapeptide (A2pm->D-ala) (middle of chain)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21249N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptideMetaboCard
ECMDB21250N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptideMetaboCard
GO Classification:
Component
cell part
membrane
Function
catalytic activity
lytic transglycosylase activity
transferase activity
transferase activity, transferring glycosyl groups
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b2963
Gene OrientationClockwise
Centisome Percentage:66.87
Left Sequence End3102455
Right Sequence End3103534
Gene Sequence:
>1080 bp
ATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATCTCCTGTTCGACGACC
AAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTG
ATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGTGATCGCT
GGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTC
GATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGC
CGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCC
GACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAAC
ACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAGCAACTTCGCGGATTATCTGCTGAAA
AACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATG
GTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCA
CGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTT
AACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACT
GCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTG
TTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTT
TATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAAC
GGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATT
ATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCG
GAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAA
Protein Properties
Pfam Domain Function:
Protein Residues:359
Protein Molecular Weight:40112
Protein Theoretical pI:10
Signaling Regions:
  • 1-16
Transmembrane Regions:
  • None
Protein Sequence:
>Membrane-bound lytic murein transglycosylase C
MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQFAHNIENIWGFKEVVIA
GPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRAIIKTLLMGDDPSSVDLYS
DVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLKNRLKSRSNGLRIIYSVTINM
VPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTESSFNPYAVSRSDALGLMQVVQHT
AGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAMLNNVYLGGIDNPTSRRYAVITAYN
GGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLTTRHPSAESRRYLYKVNTAQKSYRRR
References
External Links:
ResourceLink
Uniprot ID:P0C066
Uniprot Name:MLTC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675773
Ecogene ID:EG12986
Ecocyc:EG12986
ColiBase:b2963
Kegg Gene:b2963
EchoBASE ID:EB2810
CCDB:MLTC_ECOLI
BacMap:90111520
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Dijkstra, A. J., Keck, W. (1996). "Identification of new members of the lytic transglycosylase family in Haemophilus influenzae and Escherichia coli." Microb Drug Resist 2:141-145. Pubmed: 9158737
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Pomposiello, P. J., Koutsolioutsou, A., Carrasco, D., Demple, B. (2003). "SoxRS-regulated expression and genetic analysis of the yggX gene of Escherichia coli." J Bacteriol 185:6624-6632. Pubmed: 14594836