Identification
Name:DNA gyrase subunit B
Synonyms:Not Available
Gene Name:gyrB
Enzyme Class:
Biological Properties
General Function:Involved in ATP binding
Specific Function:DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
GO Classification:
Component
chromosome
intracellular non-membrane-bounded organelle
non-membrane-bounded organelle
organelle
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
DNA binding
DNA topoisomerase (ATP-hydrolyzing) activity
DNA topoisomerase activity
nucleic acid binding
nucleoside binding
purine nucleoside binding
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA topological change
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:b3699
Gene OrientationCounterclockwise
Centisome Percentage:83.53
Left Sequence End3875728
Right Sequence End3878142
Gene Sequence:
>2415 bp
ATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTG
GCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAG
AAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCA
CGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAG
CAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAA
GCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATCCCCCTGGCCTGAAA
CCGCAGGCGGGCAGCGTATTGCTGGCCCCACGCCTGATGGCACTGCTTAACCTGAAAACG
GGCGACACCATTGACGTGGGCGATGCCACCTTGCGGATTGCCGGAGAAGTGATTCAGGAA
CCGGATTCCGGTTTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGAT
GTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGTCACCTGGCGTTATAAATTCGGC
GGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAA
CAACGCTGGTACGGTCTGGAACAGGACGAAGGCGCGCTGGGGCGATCGATGGAACGCTCG
CAACAGTTCCTGCTGCTTTCGGCGCTTCTGACCTTGCTGCTGGCAGTGGCAGCGGTGGCG
GTAGCGATGAATCATTACTGTCGCAGTCGCTACGATCTGGTGGCGATCCTCAAAACGCTG
GGGGCAGGGCGAGCGCAACTGCGTAAGCTAATCGTCGGTCAGTGGTTGATGGTGCTGACG
CTTTCAGCCGTTACCGGTGGGGCCATAGGCCTGTTGTTCGAAAACGTGTTGATGGTGCTG
CTCAAGCCCGTTCTGCCTGCTGCACTACCGCCAGCCAGCCTCTGGCCGTGGCTGTGGGCG
CTTGGCACCATGACGGTCATCTCGCTGCTGGTGGGGCTACGACCATATCGCTTGTTGCTG
GCAACGCAGCCTTTACGCGTATTACGTAATGATGTGGTAGCGAACGTCTGGCCGCTGAAG
TTTTATCTGCCGATTGTCAGTGTGGTGGTTGTGCTGCTGCTCGCCGGATTAATGGGTGGC
AGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTG
CTGGGCTGGATGCTGCTGAATGTACTTCGCCGCATGACGCTGAAATCGCTGCCTCTGCGC
CTGGCGGTTAGCCGCCTGTTACGTCAGCCGTGGTCAACGTTAAGTCAGCTTTCGGCATTT
TCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGC
TGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAA
CAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTAT
CCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGAT
GAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCG
ATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCG
AAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTC
CGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTAT
TTTATTTTCCCTGAAGGGGCATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGC
TGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTG
TTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCG
CTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTG
CAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGG
AAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTCCGGC
CTGGTGGCCGCAATTGGTGCGGAAACGGCGCTGGCAGTGTTGCAGGCGAAAGTGTTTGAT
TTTCCGTGGGAGCCAGACTGGCGATTGTGGATTGTTCTGCCGTGCAGCGGAGCGCTGCTG
CTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGG
CAGTTTGCGGGGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:804
Protein Molecular Weight:89949
Protein Theoretical pI:6
PDB File:1EI1
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>DNA gyrase subunit B
MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEII
VTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
SVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGETEKTGTMVRFWPSLETFTNVT
EFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKAFVEYLNKNKTPIHPNI
FYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKE
GYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNELLAEY
LLENPTDAKIVVGKIIDAARAREAARRAREMTRRKGALDLAGLPGKLADCQERDPALSEL
YLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLYKVKKG
KQEQYIKDDEAMDQYQISIALDGATLHTNASAPALAGEALEKLVSEYNATQKMINRMERR
YPKAMLKELIYQPTLTEADLSDEQTVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLF
EPIVRVRTHGVDTDYPLDHEFITGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFE
QALDWLVKESRRGLSIQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTL
MGDAVEPRRAFIEENALKAANIDI
References
External Links:
ResourceLink
Uniprot ID:P0AES6
Uniprot Name:GYRB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674635
PDB ID:1EI1
Ecogene ID:EG10424
Ecocyc:EG10424
ColiBase:b3699
Kegg Gene:b3699
EchoBASE ID:EB0419
CCDB:GYRB_ECOLI
BacMap:49176395
General Reference:
  • Adachi, T., Mizuuchi, K., Menzel, R., Gellert, M. (1984). "DNA sequence and transcription of the region upstream of the E. coli gyrB gene." Nucleic Acids Res 12:6389-6395. Pubmed: 6089112
  • Adachi, T., Mizuuchi, M., Robinson, E. A., Appella, E., O'Dea, M. H., Gellert, M., Mizuuchi, K. (1987). "DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy." Nucleic Acids Res 15:771-784. Pubmed: 3029692
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
  • Funatsuki, K., Tanaka, R., Inagaki, S., Konno, H., Katoh, K., Nakamura, H. (1997). "acrB mutation located at carboxyl-terminal region of gyrase B subunit reduces DNA binding of DNA gyrase." J Biol Chem 272:13302-13308. Pubmed: 9148951
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Holdgate, G. A., Tunnicliffe, A., Ward, W. H., Weston, S. A., Rosenbrock, G., Barth, P. T., Taylor, I. W., Pauptit, R. A., Timms, D. (1997). "The entropic penalty of ordered water accounts for weaker binding of the antibiotic novobiocin to a resistant mutant of DNA gyrase: a thermodynamic and crystallographic study." Biochemistry 36:9663-9673. Pubmed: 9245398
  • Menzel, R., Gellert, M. (1987). "Fusions of the Escherichia coli gyrA and gyrB control regions to the galactokinase gene are inducible by coumermycin treatment." J Bacteriol 169:1272-1278. Pubmed: 3029031
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293
  • Tamura, J. K., Gellert, M. (1990). "Characterization of the ATP binding site on Escherichia coli DNA gyrase. Affinity labeling of Lys-103 and Lys-110 of the B subunit by pyridoxal 5'-diphospho-5'-adenosine." J Biol Chem 265:21342-21349. Pubmed: 2174443
  • Vizan, J. L., Hernandez-Chico, C., del Castillo, I., Moreno, F. (1991). "The peptide antibiotic microcin B17 induces double-strand cleavage of DNA mediated by E. coli DNA gyrase." EMBO J 10:467-476. Pubmed: 1846808
  • Wigley, D. B., Davies, G. J., Dodson, E. J., Maxwell, A., Dodson, G. (1991). "Crystal structure of an N-terminal fragment of the DNA gyrase B protein." Nature 351:624-629. Pubmed: 1646964
  • Yamagishi, J., Yoshida, H., Yamayoshi, M., Nakamura, S. (1986). "Nalidixic acid-resistant mutations of the gyrB gene of Escherichia coli." Mol Gen Genet 204:367-373. Pubmed: 3020376
  • Yoshida, H., Bogaki, M., Nakamura, M., Yamanaka, L. M., Nakamura, S. (1991). "Quinolone resistance-determining region in the DNA gyrase gyrB gene of Escherichia coli." Antimicrob Agents Chemother 35:1647-1650. Pubmed: 1656869