ECMDB

Identification

Name:

AMP nucleosidase

Synonyms:

Not Available

Gene Name:

amn

Enzyme Class:

3.2.2.4

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

Involved in regulation of AMP concentrations

Cellular Location:

Not Available

SMPDB Pathways:

adenine and adenosine salvage III PW002072

KEGG Pathways:

Purine metabolism ec00230

KEGG Reactions:

1.0

↔

1.0

1.0Adenosine monophosphate + 1.0Water ↔ 1.0Adenine + 1.0D-Ribose-5-phosphate
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Adenosine monophosphate + 1.0Water ↔ 1.0Adenine + 1.0D-Ribose-5-phosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00034	Adenine	MetaboCard
ECMDB00045	Adenosine monophosphate	MetaboCard
ECMDB02033	D-Ribose-5-phosphate	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
Process
cellular nitrogen compound metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside metabolic process

Gene Properties

Blattner:

b1982

Gene Orientation

Clockwise

Centisome Percentage:

44.25

Left Sequence End

2053085

Right Sequence End

2054539

Gene Sequence:

>1455 bp
ATGGAACATCAGAGAAAACTATTCCAGCAACGCGGCTATAGCGAAGATCTATTGCCGAAA
ACGCAAAGCCAGCGGACCTGGAAAACATTTAACTATTTTACCTTATGGATGGGTTCGGTT
CATAACGTTCCCAATTATGTGATGGTCGGCGGCTTTTTTATTCTCGGCTTGTCTACCTTT
AGTATTATGCTGGCAATTATCCTCAGCGCCTTTTTCATTGCCGCGGTAATGGTATTAAAC
GGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGT
GTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTT
GGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCG
GGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCTTAATT
ACTTTCTTAATCTTCTGGCTGGTCAACGTTGGTATAGGTTTTGGCGGTGGCAAAGTTTTA
AATAAATTCACTGCCATTCTTAACCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATT
TGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAG
AAAGCAGAAAACGGTGGCTTCCTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGG
GCGGCACCGGCGGTGAGCGCATCCGACTTTACGCAAAACGCCCACTCGTTTCGTGAGCAG
GCGCTGGGGCAAACGCTGGGTTTAGTTGTGGCCTATATTCTGTTTGCGGTCGCCGGGGTA
TGTATTATTGCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATT
GTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACA
ACTATCTCCACTAACGCGACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATT
GCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTG
ATCTGCCCGTGGAAATTAATGGAAAATCAGGACAGCATTTATCTTTTCCTCGATATTATC
GGCGGAATGCTTGGTCCGGTAATTGGTGTCATGATGGCGCATTATTTTGTGGTGATGCGC
GGACAAATTAATCTTGATGAACTGTATACCGCACCTGGCGATTATAAATATTACGATAAC
GGTTTTAACCTCACTGCGTTTTCAGTAACTCTGGTGGCCGTTATTTTATCTCTTGGCGGT
AAGTTTATTCACTTTATGGAACCGTTATCGCGTGTTTCATGGTTTGTCGGCGTCATCGTC
GCCTTTGCGGCCTACGCCTTATTAAAGAAACGTACAACAGCAGAAAAAACAGGAGAGCAA
AAAACCATAGGTTAA

Protein Properties

Pfam Domain Function:

PNP_UDP_1 (PF01048 )
AMNp_N (PF10423 )

Protein Residues:

484

Protein Molecular Weight:

53994

Protein Theoretical pI:

PDB File:

1T8Y

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>AMP nucleosidase
MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDENARKQGLFVYPSLTVT
WDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLTLLYQDYGAHISVQPSQHE
IPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETADGIYHPTEFSPLSHFDARRVD
FSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGCSQILDPDSPYIALSCAGGNWIT
AETEAPEEAISDLAWKKHQMPAWHLITADGQGITLVNIGVGPSNAKTICDHLAVLRPDVW
LMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAVLPPDIPIPSIAEVQRALYDATKLVSG
RPGEEVKQRLRTGTVVTTDDRNWELRYSASALRFNLSRAVAIDMESATIAAQGYRFRVPY
GTLLCVSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDRLHSRKLRTFNE
PPFR

References

External Links:

Resource	Link
Uniprot ID:	P0AE12
Uniprot Name:	AMN_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674649
PDB ID:	1T8Y
Ecogene ID:	EG10039
Ecocyc:	EG10039
ColiBase:	b1982
Kegg Gene:	b1982
EchoBASE ID:	EB0037
CCDB:	AMN_ECOLI
BacMap:	16129926

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
Leung, H. B., Kvalnes-Krick, K. L., Meyer, S. L., deRiel, J. K., Schramm, V. L. (1989). "Structure and regulation of the AMP nucleosidase gene (amn) from Escherichia coli." Biochemistry 28:8726-8733. Pubmed: 2690948
Whipp, M. J., Camakaris, H., Pittard, A. J. (1998). "Cloning and analysis of the shiA gene, which encodes the shikimate transport system of escherichia coli K-12." Gene 209:185-192. Pubmed: 9524262

AMP nucleosidase (P0AE12)