Identification
Name:Octaprenyl-diphosphate synthase
Synonyms:
  • Octaprenyl pyrophosphate synthase
  • OPP synthase
Gene Name:ispB
Enzyme Class:
Biological Properties
General Function:Involved in isoprenoid biosynthetic process
Specific Function:Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone
Cellular Location:Not Available
SMPDB Pathways:
  • Secondary Metabolites: Ubiquinol biosynthesis PW000981
  • Secondary Metabolites: Ubiquinol biosynthesis 2 PW002036
  • Secondary metabolites: methylerythritol phosphate and polyisoprenoid biosynthesis PW000958
KEGG Pathways:
  • Terpenoid backbone biosynthesis ec00900
KEGG Reactions:
1.0Thumb+5.0Thumb1.0Thumb+5.0Thumb
SMPDB Reactions:
1.0Farnesyl pyrophosphate+5.0Isopentenyl pyrophosphate+1.0Thumb+5.0Thumb5.0Pyrophosphate+1.0Octaprenyl diphosphate+1.0Thumb
1.0Farnesyl pyrophosphate + 5.0Isopentenyl pyrophosphate + 1.0Farnesyl pyrophosphate + 5.0Isopentenyl pyrophosphate → 5.0Pyrophosphate + 1.0Octaprenyl diphosphate + 1.0Octaprenyl diphosphate
ReactionCard
Complex Reactions:
1.0Thumb+5.0Thumb5.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB00961Farnesyl pyrophosphateMetaboCard
ECMDB01347Isopentenyl pyrophosphateMetaboCard
ECMDB01094Octaprenyl diphosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Process
cellular lipid metabolic process
isoprenoid biosynthetic process
isoprenoid metabolic process
lipid metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b3187
Gene OrientationClockwise
Centisome Percentage:71.81
Left Sequence End3331732
Right Sequence End3332703
Gene Sequence:
>972 bp
GTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATG
CGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATT
ATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATT
ACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTT
GAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT
GCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAA
TCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCA
GACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTAC
GTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGAC
GTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGATTGACCTGACCATTGTG
GCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACCGT
ATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCC
TTGACCCAGACGCTGCACCGTACCTTGATCACATCCGGTACTACCTTGATGGTTATCCTG
ATGCTGTACCTCTTCGGTGGTCCGGTACTGGAAGGCTTCTCGCTGACCATGCTTATCGGT
GTTTCCATCGGTACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGT
ATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCA
ATTCTGCCGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:323
Protein Molecular Weight:35217
Protein Theoretical pI:4
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Octaprenyl-diphosphate synthase
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVG
YEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
QMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITEENYMRVIYSKTARLFEAAAQ
CSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNADGEQLGKNVGDDLNEGKPTLPL
LHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIAALQ
VLPDTPWREALIGLAHIAVQRDR
References
External Links:
ResourceLink
Uniprot ID:P0AD57
Uniprot Name:ISPB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674549
Ecogene ID:EG10017
Ecocyc:EG10017
ColiBase:b3187
Kegg Gene:b3187
EchoBASE ID:EB0017
CCDB:ISPB_ECOLI
BacMap:16131077
General Reference:
  • Asai, K., Fujisaki, S., Nishimura, Y., Nishino, T., Okada, K., Nakagawa, T., Kawamukai, M., Matsuda, H. (1994). "The identification of Escherichia coli ispB (cel) gene encoding the octaprenyl diphosphate synthase." Biochem Biophys Res Commun 202:340-345. Pubmed: 8037730
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Choi, Y. L., Nishida, T., Kawamukai, M., Utsumi, R., Sakai, H., Komano, T. (1989). "Cloning and sequencing of an Escherichia coli gene, nlp, highly homologous to the ner genes of bacteriophages Mu and D108." J Bacteriol 171:5222-5225. Pubmed: 2670911
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jeong, J. H., Kitakawa, M., Isono, S., Isono, K. (1993). "Cloning and nucleotide sequencing of the genes, rpIU and rpmA, for ribosomal proteins L21 and L27 of Escherichia coli." DNA Seq 4:59-67. Pubmed: 8312607