Chaperone surA (P0ABZ6)

Identification
Name:Chaperone surA
Synonyms:
  • Peptidyl-prolyl cis-trans isomerase surA
  • PPIase surA
  • Rotamase surA
  • Survival protein A
Gene Name:surA
Enzyme Class:
Biological Properties
General Function:Posttranslational modification, protein turnover, chaperones
Specific Function:Chaperone involved in the correct folding and assembly of outer membrane proteins, such as ompA, ompF and lamB. It recognizes specific patterns of aromatic residues and the orientation of their side chains, which are found more frequently in integral outer membrane proteins. May act in both early periplasmic and late outer membrane-associated steps of protein maturation. Essential for the survival of E.coli in stationary phase. Required for pilus biogenesis
Cellular Location:Periplasm.
SMPDB Pathways:
  • Operon: 16S ribosomal RNA modification & chaperone PW001877
KEGG Pathways:Not Available
KEGG Reactions:
1.0Peptidylproline (omega=180) ↔ 1.0Peptidylproline (omega=0)
1.0Peptidylproline (omega=180) ↔ 1.0Peptidylproline (omega=0)
ReactionCard
Metabolites:
ECMDB IDNameView
GO Classification:
Function
catalytic activity
isomerase activity
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1287 bp
ATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACC
CAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTG
ACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCG
GCGTTTATTGGTGATGAAATCCCACAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTT
GCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCAC
GGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGACCCAT
ATTGCGGGTGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCA
GTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGC
AAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTT
GCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAA
GAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTG
GCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAG
CTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAG
TCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCA
CGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCG
ATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATC
TGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATG
CGTGAGTTAAAAGAACTGGGCTACACTTCGGAGCCGCACGCTGCCGTAGCTTATCGTGCG
CTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCG
AAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTG
GCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTG
CGTAAATTGATGATGAATCATCAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:428
Protein Molecular Weight:47283
Protein Theoretical pI:7
PDB File:1M5Y
Signaling Regions:
  • 1-20
Transmembrane Regions:
  • None
Protein Sequence:
>Chaperone surA
MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDGLMQSVKLNAAQARQQL
PDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIANIAKQNNMTLDQMRSRLAY
DGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESLAQQVGNQNDASTELNLSHIL
IPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIAHSADQQALNGGQMGWGRIQELP
GIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGESKNISVTEVHARHILLKPSPIMTD
EQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGSANQGGDLGWATPDIFDPAFRDALTRL
NKGQMSAPVHSSFGWHLIELLDTRNVDKTDAAQKDRAYRMLMNRKFSEEAASWMQEQRAS
AYVKILSN
References
External Links:
ResourceLink
Uniprot ID:P0ABZ6
Uniprot Name:SURA_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:1786185
PDB ID:1M5Y
CCDB:SURA_ECOLI
General Reference:
  • Behrens, S., Maier, R., de Cock, H., Schmid, F. X., Gross, C. A. (2001). "The SurA periplasmic PPIase lacking its parvulin domains functions in vivo and has chaperone activity." EMBO J 20:285-294. Pubmed: 11226178
  • Bitto, E., McKay, D. B. (2002). "Crystallographic structure of SurA, a molecular chaperone that facilitates folding of outer membrane porins." Structure 10:1489-1498. Pubmed: 12429090
  • Bitto, E., McKay, D. B. (2003). "The periplasmic molecular chaperone protein SurA binds a peptide motif that is characteristic of integral outer membrane proteins." J Biol Chem 278:49316-49322. Pubmed: 14506253
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hennecke, G., Nolte, J., Volkmer-Engert, R., Schneider-Mergener, J., Behrens, S. (2005). "The periplasmic chaperone SurA exploits two features characteristic of integral outer membrane proteins for selective substrate recognition." J Biol Chem 280:23540-23548. Pubmed: 15840585
  • Justice, S. S., Hunstad, D. A., Harper, J. R., Duguay, A. R., Pinkner, J. S., Bann, J., Frieden, C., Silhavy, T. J., Hultgren, S. J. (2005). "Periplasmic peptidyl prolyl cis-trans isomerases are not essential for viability, but SurA is required for pilus biogenesis in Escherichia coli." J Bacteriol 187:7680-7686. Pubmed: 16267292
  • Lazar, S. W., Kolter, R. (1996). "SurA assists the folding of Escherichia coli outer membrane proteins." J Bacteriol 178:1770-1773. Pubmed: 8626309
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Missiakas, D., Betton, J. M., Raina, S. (1996). "New components of protein folding in extracytoplasmic compartments of Escherichia coli SurA, FkpA and Skp/OmpH." Mol Microbiol 21:871-884. Pubmed: 8878048
  • Roa, B. B., Connolly, D. M., Winkler, M. E. (1989). "Overlap between pdxA and ksgA in the complex pdxA-ksgA-apaG-apaH operon of Escherichia coli K-12." J Bacteriol 171:4767-4777. Pubmed: 2670894
  • Rouviere, P. E., Gross, C. A. (1996). "SurA, a periplasmic protein with peptidyl-prolyl isomerase activity, participates in the assembly of outer membrane porins." Genes Dev 10:3170-3182. Pubmed: 8985185
  • Tormo, A., Almiron, M., Kolter, R. (1990). "surA, an Escherichia coli gene essential for survival in stationary phase." J Bacteriol 172:4339-4347. Pubmed: 2165476
  • Wilkins, M. R., Gasteiger, E., Tonella, L., Ou, K., Tyler, M., Sanchez, J. C., Gooley, A. A., Walsh, B. J., Bairoch, A., Appel, R. D., Williams, K. L., Hochstrasser, D. F. (1998). "Protein identification with N and C-terminal sequence tags in proteome projects." J Mol Biol 278:599-608. Pubmed: 9600841
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901