Poly(A) polymerase (P0ABF1)

Identification
Name:Poly(A) polymerase
Synonyms:
  • PAP
  • Plasmid copy number protein
Gene Name:pcnB
Enzyme Class:
Biological Properties
General Function:Involved in RNA binding
Specific Function:Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0RNA+1.0RNA ↔ 1.0Thumb+1.0RNA
1.0Adenosine triphosphate + 1.0RNA + 1.0RNA ↔ 1.0Pyrophosphate + 1.0RNA
ReactionCard
Complex Reactions:
1.0Thumb+1.0RNA(n) → 1.0Thumb+1.0RNA(n+1)
1.0Adenosine triphosphate + 1.0RNA(n) → 1.0Pyrophosphate + 1.0RNA(n+1)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
adenylyltransferase activity
binding
catalytic activity
nucleic acid binding
nucleotidyltransferase activity
polynucleotide adenylyltransferase activity
RNA binding
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
mRNA 3'-end processing
mRNA polyadenylation
mRNA processing
RNA metabolic process
RNA processing
Gene Properties
Blattner:b0143
Gene OrientationCounterclockwise
Centisome Percentage:3.40
Left Sequence End157729
Right Sequence End159126
Gene Sequence:
>1398 bp
ATTTTTACCCGAGTCGCTAATTTTTGCCGCAAGGTGCTAAGCCGCGAGGAAAGCGAGGCT
GAACAGGCAGTCGCCCGTCCACAGGTGACGGTGATCCCGCGTGAGCAGCATGCTATTTCC
CGCAAAGATATCAGTGAAAATGCCCTGAAGGTAATGTACAGGCTCAATAAAGCGGGATAC
GAAGCCTGGCTGGTTGGCGGCGGCGTGCGCGACCTGTTACTTGGCAAAAAGCCGAAAGAT
TTTGACGTAACCACTAACGCCACGCCTGAGCAGGTGCGCAAACTGTTCCGTAACTGCCGC
CTGGTGGGTCGCCGTTTCCGTCTGGCTCATGTAATGTTTGGCCCGGAGATTATCGAAGTT
GCGACCTTCCGTGGACACCACGAAGGTAACGTCAGCGACCGCACGACCTCCCAACGCGGG
CAAAACGGCATGTTGCTGCGCGACAACATTTTCGGCTCCATCGAAGAAGACGCCCAGCGC
CGCGATTTCACTATCAACAGCCTGTATTACAGCGTAGCGGATTTTACCGTCCGTGATTAC
GTTGGCGGCATGAAGGATCTGAAGGACGGCGTTATCCGTCTGATTGGTAACCCGGAAACG
CGCTACCGTGAAGATCCGGTACGTATGCTGCGCGCGGTACGTTTTGCCGCCAAATTGGGT
ATGCGCATCAGCCCGGAAACCGCAGAACCGATCCCTCGCCTCGCTACCCTGCTGAACGAT
ATCCCACCGGCACGCCTGTTTGAAGAATCGCTTAAACTGCTACAAGCGGGCTACGGTTAC
GAAACCTATAAGCTGTTGTGTGAATATCATCTGTTCCAGCCGCTGTTCCCGACCATTACC
CGCTACTTCACGGAAAATGGCGACAGCCCGATGGAGCGGATCATTGAACAGGTGCTGAAG
AATACCGATACGCGTATCCATAACGATATGCGCGTGAACCCGGCGTTCCTGTTTGCCGCC
ATGTTCTGGTACCCACTGCTGGAGACGGCACAGAAGATCGCCCAGGAAAGCGGCCTGACC
TATCACGACGCTTTCGCGCTGGCGATGAACGACGTGCTGGACGAAGCCTGCCGTTCACTG
GCAATCCCGAAACGTCTGACGACATTAACCCGCGATATCTGGCAGTTGCAGTTGCGTATG
TCCCGTCGTCAGGGTAAACGCGCATGGAAACTGCTGGAGCATCCTAAGTTCCGTGCGGCT
TATGACCTGTTGGCCTTGCGAGCTGAAGTTGAGCGTAACGCTGAACTGCAGCGTCTGGTG
AAATGGTGGGGTGAGTTCCAGGTTTCCGCGCCACCAGACCAAAAAGGGATGCTCAACGAG
CTGGATGAAGAACCGTCACCGCGTCGTCGTACTCGTCGTCCACGCAAACGCGCACCACGT
CGTGAGGGTACCGCATGA
Protein Properties
Pfam Domain Function:
Protein Residues:465
Protein Molecular Weight:53870
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Poly(A) polymerase
MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDISENALKVMYRLNKAGY
EAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEV
ATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDY
VGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAKLGMRISPETAEPIPRLATLLND
IPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLFPTITRYFTENGDSPMERIIEQVLK
NTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIAQESGLTYHDAFALAMNDVLDEACRSL
AIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLV
KWWGEFQVSAPPDQKGMLNELDEEPSPRRRTRRPRKRAPRREGTA
References
External Links:
ResourceLink
Uniprot ID:P0ABF1
Uniprot Name:PCNB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674351
Ecogene ID:EG10690
Ecocyc:EG10690
ColiBase:b0143
Kegg Gene:b0143
EchoBASE ID:EB0684
CCDB:PCNB_ECOLI
BacMap:162135892
General Reference:
  • Binns, N., Masters, M. (2002). "Expression of the Escherichia coli pcnB gene is translationally limited using an inefficient start codon: a second chromosomal example of translation initiated at AUU." Mol Microbiol 44:1287-1298. Pubmed: 12068810
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cao, G. J., Sarkar, N. (1992). "Identification of the gene for an Escherichia coli poly(A) polymerase." Proc Natl Acad Sci U S A 89:10380-10384. Pubmed: 1438224
  • Fujita, N., Mori, H., Yura, T., Ishihama, A. (1994). "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Nucleic Acids Res 22:1637-1639. Pubmed: 8202364
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Liu, J. D., Parkinson, J. S. (1989). "Genetics and sequence analysis of the pcnB locus, an Escherichia coli gene involved in plasmid copy number control." J Bacteriol 171:1254-1261. Pubmed: 2537812
  • Masters, M., March, J. B., Oliver, I. R., Collins, J. F. (1990). "A possible role for the pcnB gene product of Escherichia coli in modulating RNA: RNA interactions." Mol Gen Genet 220:341-344. Pubmed: 1691435
  • Raynal, L. C., Carpousis, A. J. (1999). "Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation." Mol Microbiol 32:765-775. Pubmed: 10361280