Probable diguanylate cyclase AdrA (P0AAP1)

Identification
Name:Probable diguanylate cyclase AdrA
Synonyms:
  • DGC
Gene Name:adrA
Enzyme Class:
Biological Properties
General Function:Signal transduction mechanisms
Specific Function:A probable diguanylate cyclase. The last member of a cascade of expressed proteins, its expression requires YdaM. AdrA production induces biosynthesis of cellulose in some E.coli isolates, but not in K12 strains. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria
Cellular Location:Cell inner membrane; Multi-pass membrane protein (Potential)
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
2.0Thumb ↔ 1.03',5'-Cyclic diGMP+2.0Thumb+1.0Thumb
2.0Guanosine triphosphate ↔ 1.03',5'-Cyclic diGMP + 2.0Pyrophosphate + 1.0Cyclic di-3',5'-guanylate
ReactionCard
Complex Reactions:
2.0Thumb → 2.0Thumb+1.0Thumb
2.0Guanosine triphosphate → 2.0Pyrophosphate + 1.0Cyclic di-3',5'-guanylate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB23177Cyclic di-3',5'-guanylateMetaboCard
ECMDB01273Guanosine triphosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1116 bp
ATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAG
GAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGC
GTCAGACTACCCCGTGCGGTTGGCCTGGCTGGCATGTTCTTACCGATTGCTTCAACGCTG
GTTTCACACCCGCCGCCGGGCTGGTGGTGGCTGGTGTTGGTCGGCTGGGCGTTCGTCTGG
CCGCATTTAGCCTGGCAGATAGCGAGCAGGGCCGTCGATCCGCTTAGCCGGGAAATTTAC
AACTTAAAAACCGATGCAGTATTAGCGGGAATGTGGGTAGGCGTAATGGGCGTAAACGTG
CTGCCTTCCACCGCGATGTTGATGATTATGTGTCTGAATTTGATGGGGGCAGGCGGCCCC
CGTCTGTTTGTCGCGGGTCTGGTGTTGATGGTGGTTTCCTGCCTTGTCACCCTCGAGCTG
ACGGGCATTACCGTGTCGTTCAATAGTGCGCCGCTGGAATGGTGGCTCTCCCTTCCCATT
ATTGTCATTTATCCTCTGCTGTTTGGCTGGGTCAGCTACCAGACGGCAACCAAACTGGCG
GAACATAAACGCAGGTTGCAGGTCATGAGTACCCGCGACGGCATGACGGGCGTGTATAAC
CGACGTCATTGGGAAACTATGTTACGCAATGAATTTGATAACTGTCGGCGGCATAATCGC
GATGCAACGTTACTGATTATCGATATCGACCATTTCAAGAGCATCAACGATACCTGGGGC
CATGATGTGGGCGATGAAGCGATTGTGGCGCTTACCCGACAGTTACAAATTACCCTGCGC
GGTAGCGATGTGATTGGTCGGTTTGGCGGCGATGAGTTTGCAGTAATCATGTCCGGTACG
CCAGCTGAGAGCGCCATTACCGCCATGTTACGGGTGCATGAAGGGCTAAATACATTACGT
TTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCA
CAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAG
AAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:371
Protein Molecular Weight:41537
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • 46-66
  • 68-88
  • 112-132
  • 143-163
  • 171-191
Protein Sequence:
>Probable diguanylate cyclase AdrA
MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLPRAVGLAGMFLPIASTL
VSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNLKTDAVLAGMWVGVMGVNV
LPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLELTGITVSFNSAPLEWWLSLPI
IVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGVYNRRHWETMLRNEFDNCRRHNR
DATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQITLRGSDVIGRFGGDEFAVIMSGT
PAESAITAMLRVHEGLNTLRLPNTPQVTLRISVGVAPLNPQMSHYREWLKSADLALYKAK
KAGRNRTEVAA
References
External Links:
ResourceLink
Uniprot ID:P0AAP1
Uniprot Name:ADRA_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:1786584
CCDB:ADRA_ECOLI
General Reference:
  • Antoniani, D., Bocci, P., Maciag, A., Raffaelli, N., Landini, P. (2010). "Monitoring of diguanylate cyclase activity and of cyclic-di-GMP biosynthesis by whole-cell assays suitable for high-throughput screening of biofilm inhibitors." Appl Microbiol Biotechnol 85:1095-1104. Pubmed: 19707751
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Brombacher, E., Baratto, A., Dorel, C., Landini, P. (2006). "Gene expression regulation by the Curli activator CsgD protein: modulation of cellulose biosynthesis and control of negative determinants for microbial adhesion." J Bacteriol 188:2027-2037. Pubmed: 16513732
  • Chang, C. N., Kuang, W. J., Chen, E. Y. (1986). "Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli." Gene 44:121-125. Pubmed: 3533724
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Sommerfeldt, N., Possling, A., Becker, G., Pesavento, C., Tschowri, N., Hengge, R. (2009). "Gene expression patterns and differential input into curli fimbriae regulation of all GGDEF/EAL domain proteins in Escherichia coli." Microbiology 155:1318-1331. Pubmed: 19332833
  • Weber, H., Pesavento, C., Possling, A., Tischendorf, G., Hengge, R. (2006). "Cyclic-di-GMP-mediated signalling within the sigma network of Escherichia coli." Mol Microbiol 62:1014-1034. Pubmed: 17010156
  • Zogaj, X., Nimtz, M., Rohde, M., Bokranz, W., Romling, U. (2001). "The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix." Mol Microbiol 39:1452-1463. Pubmed: 11260463