Membrane-bound lytic murein transglycosylase A (P0A935)

Identification
Name:Membrane-bound lytic murein transglycosylase A
Synonyms:
  • Mlt38
  • Murein hydrolase A
Gene Name:mltA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in hydrolase activity, hydrolyzing O-glycosyl compounds
Specific Function:Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Degrades murein glycan strands and insoluble, high-molecular weight murein sacculi
Cellular Location:Cell outer membrane; Lipid-anchor
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
EcoCyc Reactions:
1.0a peptidoglycan polymer ? 1.0a peptidoglycan polymer with 1,6-anhydromuropeptide end+1.0a peptidoglycan polymer with GlcNAc end
1.0a peptidoglycan polymer ? 1.0a peptidoglycan polymer with 1,6-anhydromuropeptide end + 1.0a peptidoglycan polymer with GlcNAc end
ReactionCard
Complex Reactions:
1.0two linked disacharide tetrapeptide and tripeptide murein units (uncrosslinked, middle of chain) → 1.0Thumb+1.0Thumb
1.0two linked disacharide tetrapeptide and tripeptide murein units (uncrosslinked, middle of chain) → 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide + 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide
ReactionCard
1.0two linked disacharide tripeptide murein units (uncrosslinked, middle of chain) → 2.0Thumb
1.0two linked disacharide tripeptide murein units (uncrosslinked, middle of chain) → 2.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide
ReactionCard
1.0two linked disacharide tetrapeptide murein units (uncrosslinked, middle of chain) → 2.0Thumb
1.0two linked disacharide tetrapeptide murein units (uncrosslinked, middle of chain) → 2.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide
ReactionCard
1.0three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala), one uncrosslinked tetrapaptide) (middle of chain) → 1.0Thumb+1.0two disacharide linked murein units, tetrapeptide corsslinked tetrapeptide (A2pm->D-ala) (middle of chain)
1.0three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala), one uncrosslinked tetrapaptide) (middle of chain) → 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide + 1.0two disacharide linked murein units, tetrapeptide corsslinked tetrapeptide (A2pm->D-ala) (middle of chain)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21249N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptideMetaboCard
ECMDB21250N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptideMetaboCard
GO Classification:
Component
cell part
membrane
outer membrane
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing O-glycosyl compounds
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan metabolic process
peptidoglycan turnover
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b2813
Gene OrientationCounterclockwise
Centisome Percentage:63.45
Left Sequence End2944103
Right Sequence End2945200
Gene Sequence:
>1098 bp
GTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGACGGCGGCTCAGGCTGAG
CGTATTCGCGATCTCACCAGTGTTCAGGGGGTAAGGCAAAACTCACTGATTGGCTATGGT
CTGGTGGTGGGGCTGGATGGCACCGGTGACCAGACAACCCAGACGCCGTTTACCACACAA
ACGCTTAATAACATGCTCTCACAGCTGGGAATTACCGTTCCGACGGGCACCAATATGCAG
CTAAAAAACGTCGCTGCGGTAATGGTGACAGCGTCACTTCCTCCGTTTGGACGTCAGGGG
CAAACCATCGATGTGGTGGTTTCTTCCATGGGAAATGCCAAAAGCTTGCGTGGAGGTACG
TTGTTGATGACACCGCTTAAGGGCGTTGACAGTCAGGTGTATGCGCTGGCGCAGGGCAAT
ATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTG
AACGGTGGACGGATCACCAATGGTGCGGTTATTGAACGTGAATTGCCCAGCCAGTTTGGC
GTCGGGAATACCCTTAATTTGCAACTTAACGACGAAGATTTCAGCATGGCGCAGCAAATC
GCTGACACCATCAACCGCGTGCGTGGATATGGCAGCGCCACCGCGTTAGATGCGCGGACT
ATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAG
AATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGT
TCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTC
TCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGA
CAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAA
AGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCT
ACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCA
AAACTGGAAATCATCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:365
Protein Molecular Weight:40410
Protein Theoretical pI:9
Signaling Regions:
  • 1-20
Transmembrane Regions:
  • None
Protein Sequence:
>Membrane-bound lytic murein transglycosylase A
MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLVNQPDAVGAPINAGDFA
EQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGIDAWQMEGADNYGNVQFTG
YYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGALSDKYILAYSNSLMDNFIMD
VQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDRGEVKKEDMSMQAIRHWGETHSE
AEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRASVASDRSIIPPGTTLLAEVPLLDN
NGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIGPEAGHRAGWYNHYGRVWVLKTAPGAG
NVFSG
References
External Links:
ResourceLink
Uniprot ID:P0A935
Uniprot Name:MLTA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062658
Ecogene ID:EG13085
Ecocyc:EG13085
ColiBase:b2813
Kegg Gene:b2813
EchoBASE ID:EB2894
CCDB:MLTA_ECOLI
BacMap:16130720
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lommatzsch, J., Templin, M. F., Kraft, A. R., Vollmer, W., Holtje, J. V. (1997). "Outer membrane localization of murein hydrolases: MltA, a third lipoprotein lytic transglycosylase in Escherichia coli." J Bacteriol 179:5465-5470. Pubmed: 9287002
  • Ursinus, A., Holtje, J. V. (1994). "Purification and properties of a membrane-bound lytic transglycosylase from Escherichia coli." J Bacteriol 176:338-343. Pubmed: 8288527
  • Vollmer, W., von Rechenberg, M., Holtje, J. V. (1999). "Demonstration of molecular interactions between the murein polymerase PBP1B, the lytic transglycosylase MltA, and the scaffolding protein MipA of Escherichia coli." J Biol Chem 274:6726-6734. Pubmed: 10037771