Outer membrane protein A (P0A910)

Identification
Name:Outer membrane protein A
Synonyms:
  • Outer membrane protein II*
Gene Name:ompA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in structural molecule activity
Specific Function:Required for the action of colicins K and L and for the stabilization of mating aggregates in conjugation. Serves as a receptor for a number of T-even like phages. Also acts as a porin with low permeability that allows slow penetration of small solutes
Cellular Location:Cell outer membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell outer membrane
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
outer membrane
Function
structural molecule activity
Gene Properties
Blattner:b0957
Gene OrientationCounterclockwise
Centisome Percentage:21.95
Left Sequence End1018236
Right Sequence End1019276
Gene Sequence:
>1041 bp
ATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTG
CTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTG
CAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGC
CCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAG
GTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCG
GCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTA
AAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAG
CGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGTTT
TACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTG
CGCGATGCCGGGATCAAAGTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAA
GATCGCGCCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCA
ATCAACATGCTGGTGAAGGTGAAAGGCACGCCGCTTGCCGATAACGATGATGTCGATGCC
TTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGC
CTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGC
GCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAA
GACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGG
GATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATAT
TACAACGCGGCAGCATTATGA
Protein Properties
Pfam Domain Function:
Protein Residues:346
Protein Molecular Weight:37201
Protein Theoretical pI:6
PDB File:1QJP
Signaling Regions:
  • 1-21
Transmembrane Regions:
  • 27-37
  • 55-66
  • 70-78
  • 96-107
  • 112-124
  • 138-151
  • 156-163
  • 182-190
Protein Sequence:
>Outer membrane protein A
MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGFINNNGPTHENQLGAGA
FGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTAKLGYPITDDLDIYTRLGG
MVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRLEYQWTNNIGDAHTIGTRPDN
GMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLKSDVLFNFNKATLKPEGQAALDQ
LYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPADKISARGM
GESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVKGIKDVVTQPQA
References
External Links:
ResourceLink
Uniprot ID:P0A910
Uniprot Name:OMPA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674758
PDB ID:1QJP
Ecogene ID:EG10669
Ecocyc:EG10669
ColiBase:b0957
Kegg Gene:b0957
EchoBASE ID:EB0663
CCDB:OMPA_ECOLI
BacMap:16128924
General Reference:
  • Arora, A., Abildgaard, F., Bushweller, J. H., Tamm, L. K. (2001). "Structure of outer membrane protein A transmembrane domain by NMR spectroscopy." Nat Struct Biol 8:334-338. Pubmed: 11276254
  • Beck, E., Bremer, E. (1980). "Nucleotide sequence of the gene ompA coding the outer membrane protein II of Escherichia coli K-12." Nucleic Acids Res 8:3011-3027. Pubmed: 6253901
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Chen, R., Schmidmayr, W., Kramer, C., Chen-Schmeisser, U., Henning, U. (1980). "Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12." Proc Natl Acad Sci U S A 77:4592-4596. Pubmed: 7001461
  • Gromiha, M. M., Ponnuswamy, P. K. (1993). "Prediction of transmembrane beta-strands from hydrophobic characteristics of proteins." Int J Pept Protein Res 42:420-431. Pubmed: 8106193
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Koebnik, R. (1999). "Structural and functional roles of the surface-exposed loops of the beta-barrel membrane protein OmpA from Escherichia coli." J Bacteriol 181:3688-3694. Pubmed: 10368142
  • Kuhn, A., Kiefer, D., Kohne, C., Zhu, H. Y., Tschantz, W. R., Dalbey, R. E. (1994). "Evidence for a loop-like insertion mechanism of pro-Omp A into the inner membrane of Escherichia coli." Eur J Biochem 226:891-897. Pubmed: 7813480
  • le Coutre, J., Whitelegge, J. P., Gross, A., Turk, E., Wright, E. M., Kaback, H. R., Faull, K. F. (2000). "Proteomics on full-length membrane proteins using mass spectrometry." Biochemistry 39:4237-4242. Pubmed: 10757971
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Molloy, M. P., Herbert, B. R., Walsh, B. J., Tyler, M. I., Traini, M., Sanchez, J. C., Hochstrasser, D. F., Williams, K. L., Gooley, A. A. (1998). "Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis." Electrophoresis 19:837-844. Pubmed: 9629924
  • Morona, R., Klose, M., Henning, U. (1984). "Escherichia coli K-12 outer membrane protein (OmpA) as a bacteriophage receptor: analysis of mutant genes expressing altered proteins." J Bacteriol 159:570-578. Pubmed: 6086577
  • Morona, R., Kramer, C., Henning, U. (1985). "Bacteriophage receptor area of outer membrane protein OmpA of Escherichia coli K-12." J Bacteriol 164:539-543. Pubmed: 3902787
  • Movva, N. R., Nakamura, K., Inouye, M. (1980). "Gene structure of the OmpA protein, a major surface protein of Escherichia coli required for cell-cell interaction." J Mol Biol 143:317-328. Pubmed: 6260961
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Pautsch, A., Schulz, G. E. (1998). "Structure of the outer membrane protein A transmembrane domain." Nat Struct Biol 5:1013-1017. Pubmed: 9808047
  • Pautsch, A., Schulz, G. E. (2000). "High-resolution structure of the OmpA membrane domain." J Mol Biol 298:273-282. Pubmed: 10764596
  • Stenberg, F., Chovanec, P., Maslen, S. L., Robinson, C. V., Ilag, L. L., von Heijne, G., Daley, D. O. (2005). "Protein complexes of the Escherichia coli cell envelope." J Biol Chem 280:34409-34419. Pubmed: 16079137
  • Sugawara, E., Nikaido, H. (1992). "Pore-forming activity of OmpA protein of Escherichia coli." J Biol Chem 267:2507-2511. Pubmed: 1370823