ECMDB

Identification

Name:

DNA-directed RNA polymerase subunit beta'

Synonyms:

RNAP subunit beta'
RNA polymerase subunit beta'
Transcriptase subunit beta'

Gene Name:

rpoC

Enzyme Class:

2.7.7.6

Biological Properties

General Function:

Involved in DNA binding

Specific Function:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Cellular Location:

Cytoplasmic

SMPDB Pathways:

Lac Operon PW000955
Lac Operon Inactivation 2 PW000956
Operon: 16S ribosomal RNA modification & chaperone PW001877
Operon: Carbamoyl-phosphate synthetase PW001874
Operon: DNA biosynthesis/heat shock protein PW001872
Operon: Elongation factor PW000990
Operon: Fix, redox process PW001876
Operon: General Secretory pathway PW000992
Operon: Iron superoxide dismutase PW000991
Operon: acetolactate synthase III activation PW001881
Operon: arabinose activation PW001878
Operon: cytidyl pyrophosphate biosynthesis of isoprenoids PW000988
Operon: leucine biosynthesis PW001880
Operon: murGC , peptidoglycan synthesis PW001883
Operon: probable carnitine operon PW001875
Operon: tRNA synthetase & peptidase PW001873
Pyrimidine metabolism PW000942
Trp Operon PW000965
Trp Operon Inactivation PW000966

KEGG Pathways:

Metabolic pathways eco01100
Purine metabolism ec00230
Pyrimidine metabolism ec00240

KEGG Reactions:

1.0

1.0RNA

↔

1.0

1.0RNA

1.0Adenosine triphosphate + 1.0RNA + 1.0RNA ↔ 1.0Pyrophosphate + 1.0RNA
ReactionCard

1.0

1.0RNA

↔

1.0

1.0RNA

1.0Guanosine triphosphate + 1.0RNA ↔ 1.0Pyrophosphate + 1.0RNA
ReactionCard

1.0

1.0RNA

↔

1.0

1.0RNA

1.0Cytidine triphosphate + 1.0RNA ↔ 1.0Pyrophosphate + 1.0RNA
ReactionCard

1.0

1.0RNA

↔

1.0

1.0RNA

1.0Uridine triphosphate + 1.0RNA ↔ 1.0Pyrophosphate + 1.0RNA
ReactionCard

1.0Nucleoside triphosphate

1.0RNA

↔

1.0

1.0Nucleoside triphosphate + 1.0RNA ↔ 1.0Pyrophosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB00082	Cytidine triphosphate	MetaboCard
ECMDB01273	Guanosine triphosphate	MetaboCard
ECMDB04142	Pyrophosphate	MetaboCard
ECMDB00285	Uridine triphosphate	MetaboCard

GO Classification:

Function
binding
catalytic activity
DNA binding
DNA-directed RNA polymerase activity
nucleic acid binding
nucleotidyltransferase activity
RNA polymerase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
biosynthetic process
cellular macromolecule biosynthetic process
macromolecule biosynthetic process
metabolic process
transcription

Gene Properties

Blattner:

b3988

Gene Orientation

Clockwise

Centisome Percentage:

90.17

Left Sequence End

4183373

Right Sequence End

4187596

Gene Sequence:

>4224 bp
GTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATC
AAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAG
CCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGT
ATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACAC
CGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAG
CGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTG
CCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTAC
TTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTG
ACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATG
GGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAG
CTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGT
ATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACC
GTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTC
GCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAA
CGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAG
GAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAAC
AAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAG
AACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATAC
CTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTC
ATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATG
GTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCG
GTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTA
CTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGAC
TTCGATGGTGACCAGATGGCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCG
CGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATC
GTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCC
AAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGT
CTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAAC
GGTGAATTAGTAGCGAAAACCAGCCTGAAAGACACGACTGTTGGCCGTGCCATTCTGTGG
ATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCA
ATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTT
GCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGT
ATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAA
GTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAAC
AAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAAC
CTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAAC
AGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTT
GCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACC
GCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGT
AAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTG
GTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATC
ATGATGACTCCGGTTATCGAGGGTGGTGACGTTAAAGAGCCGCTGCGCGATCGCGTACTG
GGTCGTGTAACTGCTGAAGACGTTCTGAAGCCGGGTACTGCTGATATCCTCGTTCCGCGC
AACACGCTGCTGCACGAACAGTGGTGTGACCTGCTGGAAGAGAACTCTGTCGACGCGGTT
AAAGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGT
CGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCA
CAGTCCATCGGTGAACCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCG
GCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTC
AGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACT
GAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGT
GCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGG
GACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATG
ATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTG
GTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATC
GTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTC
CTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACC
CTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGC
GTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGC
GGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTA
GACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAA
GGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATT
CTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTA
TACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATG
CTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTT
GAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCA
ACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATC
TCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAA
CGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGT
ACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCT
GCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGT
CTGGGCGGTTCTGATAACGAGTAA

Protein Properties

Pfam Domain Function:

RNA_pol_Rpb1_1 (PF04997 )
RNA_pol_Rpb1_2 (PF00623 )
RNA_pol_Rpb1_3 (PF04983 )
RNA_pol_Rpb1_4 (PF05000 )
RNA_pol_Rpb1_5 (PF04998 )

Protein Residues:

1407

Protein Molecular Weight:

155159

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>DNA-directed RNA polymerase subunit beta'
MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCAR
IFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERMGHIELASPTAHIWFLKSL
PSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQILTEEQYLDALEEFGDEFDAKM
GAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLTKRIKLLEAFVQSGNKPEWMILT
VLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQ
EAVDALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPY
LRLHQCGLPKKMALELFKPFIYGKLELRGLATTIKAAKKMVEREEAVVWDILDEVIREHP
VLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQMAVHVPLTLEAQLEA
RALMMSTNNILSPANGEPIIVPSQDVVLGLYYMTRDCVNAKGEGMVLTGPKEAERLYRSG
LASLHARVKVRITEYEKDANGELVAKTSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKA
ISKMLNTCYRILGLKPTVIFADQIMYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAE
VAEIQEQFQSGLVTAGERYNKVIDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFN
SIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGAR
KGLADTALKTANSGYLTRRLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVL
GRVTAEDVLKPGTADILVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYG
RDLARGHIINKGEAIGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKL
SNVKSVVNSSGKLVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANW
DPHTMPVITEVSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKI
VDAQGNDVLIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPR
VADLFEARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFE
GERVERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQM
LRKATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEAPA
APQVTAEDASASLAELLNAGLGGSDNE

References

External Links:

Resource	Link
Uniprot ID:	P0A8T7
Uniprot Name:	RPOC_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85676082
Ecogene ID:	EG10895
Ecocyc:	EG10895
ColiBase:	b3988
Kegg Gene:	b3988
EchoBASE ID:	EB0888
CCDB:	RPOC_ECOLI
BacMap:	16131818

General Reference:

Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Ovchinnikov, Y. A., Monastyrskaya, G. S., Gubanov, V. V., Guryev, S. O., Chertov, O. Y. u., Modyanov, N. N., Grinkevich, V. A., Makarova, I. A., Marchenko, T. V., Polovnikova, I. N., Lipkin, V. M., Sverdlov, E. D. (1981). "The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit." Eur J Biochem 116:621-629. Pubmed: 6266829
Ovchinnikov, Y. u. A., Monastyrskaya, G. S., Gubanov, V. V., Guryev, S. O., Salomatina, I. S., Shuvaeva, T. M., Lipkin, V. M., Sverdlov, E. D. (1982). "The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit." Nucleic Acids Res 10:4035-4044. Pubmed: 6287430
Squires, C., Krainer, A., Barry, G., Shen, W. F., Squires, C. L. (1981). "Nucleotide sequence at the end of the gene for the RNA polymerase beta' subunit (rpoC)." Nucleic Acids Res 9:6827-6840. Pubmed: 6278450
Zhang, J., Sprung, R., Pei, J., Tan, X., Kim, S., Zhu, H., Liu, C. F., Grishin, N. V., Zhao, Y. (2009). "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Mol Cell Proteomics 8:215-225. Pubmed: 18723842

DNA-directed RNA polymerase subunit beta' (P0A8T7)