Uncharacterized protein YjeA (P0A8N7)

Identification
Name:Uncharacterized protein YjeA
Synonyms:
  • GX
Gene Name:poxA
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:With YjeK might be involved in the post-translational modification of elongation factor P (EF-P)
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0tRNA(Lys)+1.0tRNA(Lys) ↔ 1.0Thumb+1.0Thumb+1.0L-Lysyl-tRNA+1.0Thumb
1.0Adenosine triphosphate + 1.0L-Lysine + 1.0tRNA(Lys) + 1.0tRNA(Lys) ↔ 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0L-Lysyl-tRNA + 1.0L-Lysyl-tRNA
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB00182L-LysineMetaboCard
ECMDB23777L-Lysyl-tRNAMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
aminoacyl-tRNA ligase activity
ATP binding
binding
catalytic activity
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
ligase activity, forming carbon-oxygen bonds
lysine-tRNA ligase activity
nucleoside binding
nucleotide binding
purine nucleoside binding
Process
biosynthetic process
cellular macromolecule biosynthetic process
cellular macromolecule metabolic process
lysyl-tRNA aminoacylation
macromolecule biosynthetic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
translation
tRNA aminoacylation
tRNA aminoacylation for protein translation
tRNA metabolic process
Gene Properties
Blattner:b4155
Gene OrientationClockwise
Centisome Percentage:94.42
Left Sequence End4380666
Right Sequence End4381643
Gene Sequence:
>978 bp
ATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGT
CTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAG
ACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAT
CCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGG
GCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCTT
GAGTCCTTCAGCGACAGTTTGTTTGATCTTCTCAATTATTACGATATGCAACTCATTGGC
GGCGATACCACGCGTGGGCCATTATCAATGACGTTGGGTATCCACGGCTTTGTTCCGATG
GGACGAGCCTTAACGCGCTCTGGGGCGAAACCGGGTGACTGGATCTATGTGACCGGTACA
CCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCT
AAAGATGCGGACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGG
CAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATCTCTCTGACGGTTTGATTTCCGAT
CTCGGGCATATCGTGAAAGCCAGCGACTGCGGCGCACGTATTGACCTGGCATTGCTGCCG
TTTTCTGATGCGCTTTCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCT
GGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGAT
GTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATC
GAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGAC
CATTTTGCCACGCCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:325
Protein Molecular Weight:36976
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Uncharacterized protein YjeA
MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLVPFETRF
VGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNEEMGRYHNPEFTMLEWYRP
HYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEIDPLSADKTQLREVAAKLDLSN
VADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPASQASLAQISTEDHRVAERFEVYY
KGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQHPIDQNLIEALKVGMPDCSGVALG
VDRLVMLALGAETLAEVIAFSVDRA
References
External Links:
ResourceLink
Uniprot ID:P0A8N7
Uniprot Name:YJEA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674557
Ecogene ID:EG11211
Ecocyc:EG11211
ColiBase:b4155
Kegg Gene:b4155
EchoBASE ID:EB1196
CCDB:YJEA_ECOLI
BacMap:90111694
General Reference:
  • Bailly, M., de Crecy-Lagard, V. (2010). "Predicting the pathway involved in post-translational modification of elongation factor P in a subset of bacterial species." Biol Direct 5:3. Pubmed: 20070887
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Chang, Y. Y., Cronan, J. E. Jr (1982). "Mapping nonselectable genes of Escherichia coli by using transposon Tn10: location of a gene affecting pyruvate oxidase." J Bacteriol 151:1279-1289. Pubmed: 6286595
  • Cole, S. T. (1982). "Nucleotide sequence coding for the flavoprotein subunit of the fumarate reductase of Escherichia coli." Eur J Biochem 122:479-484. Pubmed: 7037404
  • Gampel, A., Tzagoloff, A. (1989). "Homology of aspartyl- and lysyl-tRNA synthetases." Proc Natl Acad Sci U S A 86:6023-6027. Pubmed: 2668951
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kong, L., Fromant, M., Blanquet, S., Plateau, P. (1991). "Evidence for a new Escherichia coli protein resembling a lysyl-tRNA synthetase." Gene 108:163-164. Pubmed: 1761227