Identification |
---|
Name: | Lysyl-tRNA synthetase |
---|
Synonyms: | - Lysine--tRNA ligase
- LysRS
|
---|
Gene Name: | lysS |
---|
Enzyme Class: | |
---|
Biological Properties |
---|
General Function: | Involved in nucleotide binding |
---|
Specific Function: | ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys) |
---|
Cellular Location: | Cytoplasm |
---|
SMPDB Pathways: | Not Available |
---|
KEGG Pathways: | - Aminoacyl-tRNA biosynthesis ec00970
|
---|
KEGG Reactions: | |
1.0 | + | 1.0 | + | 1.0tRNA(Lys) | + | 1.0tRNA(Lys) | ↔ | 1.0 | + | 1.0 | + | 1.0L-Lysyl-tRNA | + | 1.0 |
| |
|
---|
Complex Reactions: | |
1.0 | + | 1.0 | + | 1.0tRNA(Lys) | → | 1.0 | + | 1.0L-Lysine-tRNA (Lys) | + | 1.0 |
| | |
1.0 | + | 1.0 | + | 1.0tRNA(Lys) | → | 1.0 | + | 1.0 | + | 1.0L-lysyl-tRNA(Lys) |
| |
|
---|
Metabolites: | |
---|
GO Classification: | Component |
---|
cell part | cytoplasm | intracellular part | Function |
---|
adenyl nucleotide binding | adenyl ribonucleotide binding | aminoacyl-tRNA ligase activity | ATP binding | binding | catalytic activity | ligase activity | ligase activity, forming aminoacyl-tRNA and related compounds | ligase activity, forming carbon-oxygen bonds | lysine-tRNA ligase activity | nucleic acid binding | nucleoside binding | nucleotide binding | purine nucleoside binding | Process |
---|
biosynthetic process | cellular macromolecule biosynthetic process | cellular macromolecule metabolic process | lysyl-tRNA aminoacylation | macromolecule biosynthetic process | macromolecule metabolic process | metabolic process | ncRNA metabolic process | RNA metabolic process | translation | tRNA aminoacylation | tRNA aminoacylation for protein translation | tRNA metabolic process |
|
---|
Gene Properties |
---|
Blattner: | b2890 |
---|
Gene Orientation | Counterclockwise |
---|
Centisome Percentage: | 65.34 |
---|
Left Sequence End | 3031679 |
---|
Right Sequence End | 3033196 |
---|
Gene Sequence: | >1518 bp
ATGGCACAGATTGATTTCCGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAG
GGCGTTAAAACCGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATC
AACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAAT
GCCGCCGTGCGCACGCGTCTTACCCACTCGATGGAAGTCCAGCAGGTGGGGCGCTACATC
GCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCATACGGCCTGGAT
GAACTGACCGGTCCCTTTGAAAGCATTGTTGAGATGTCATGCCTGATGCACGATATCGGC
AATCCGCCGTTTGGTCATTTTGGCGAAGCGGCGATAAATGACTGGTTTCGCCAACGTTTG
CACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTA
CGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTA
TGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAAT
CTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGC
GAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAA
GCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTA
ACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCG
GTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGC
CAGCATGAGAAAGGTTCGCTCTTTTCGCTGGTGGTTGAAAATGCCTGGGAAAAATCACGC
TCAAATAGTTTAAGCCGCAGTACGGAAGATCAGTTTTTTATGTATTTACGGGTAAACACC
CTAAATAAACTGGTACCCTACGCGGCACAACGATTTATTGATAATCTGCCTGCGATTTTC
GCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAG
CTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAA
TTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTG
TCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGTTTCCCTATTGAA
TCGCGCTTATTCCACAAACTCTCGACGCGCCATCGGCTGGCCTATGTCGAGGCTGTCAGT
AAATTACCGTCAGATTCTCCTGAGTTTCCGCTATGGGAATATTATTACCGTTGCCGCCTG
CTGCAGGATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTG
ATGGCCGTAGAACAATAA |
---|
Protein Properties |
---|
Pfam Domain Function: | |
---|
Protein Residues: | 505 |
---|
Protein Molecular Weight: | 57603 |
---|
Protein Theoretical pI: | 5 |
---|
PDB File: | 1BBW |
Signaling Regions: | |
---|
Transmembrane Regions: | |
---|
Protein Sequence: | >Lysyl-tRNA synthetase
MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDHTSDQLHAEFDGKENEE
LEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARDDLPEGVYNEQFKKWDLGD
ILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGLQDQEARYRQRYLDLISNDES
RNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGAAARPFITHHNALDLDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLIELTESLFRT
LAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIKKYRPETDMADLDNFDSAKAIAESIGI
HVEKSWGLGRIVTEIFEEVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFFIGG
REIGNGFSELNDAEDQAQRFLDQVAAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID
RMVMLFTNSHTIRDVILFPAMRPVK |
---|
References |
---|
External Links: | |
---|
General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Commans, S., Plateau, P., Blanquet, S., Dardel, F. (1995). "Solution structure of the anticodon-binding domain of Escherichia coli lysyl-tRNA synthetase and studies of its interaction with tRNA(Lys)." J Mol Biol 253:100-113. Pubmed: 7473706
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Kawakami, K., Jonsson, Y. H., Bjork, G. R., Ikeda, H., Nakamura, Y. (1988). "Chromosomal location and structure of the operon encoding peptide-chain-release factor 2 of Escherichia coli." Proc Natl Acad Sci U S A 85:5620-5624. Pubmed: 2456575
- Leveque, F., Plateau, P., Dessen, P., Blanquet, S. (1990). "Homology of lysS and lysU, the two Escherichia coli genes encoding distinct lysyl-tRNA synthetase species." Nucleic Acids Res 18:305-312. Pubmed: 2183178
|
---|