DNA-directed RNA polymerase subunit alpha (P0A7Z4)

Identification
Name:DNA-directed RNA polymerase subunit alpha
Synonyms:
  • RNAP subunit alpha
  • RNA polymerase subunit alpha
  • Transcriptase subunit alpha
Gene Name:rpoA
Enzyme Class:
Biological Properties
General Function:Involved in DNA-directed RNA polymerase activity
Specific Function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0RNA+1.0RNA ↔ 1.0Thumb+1.0RNA
1.0Adenosine triphosphate + 1.0RNA + 1.0RNA ↔ 1.0Pyrophosphate + 1.0RNA
ReactionCard
1.0Thumb+1.0RNA ↔ 1.0Thumb+1.0RNA
1.0Guanosine triphosphate + 1.0RNA ↔ 1.0Pyrophosphate + 1.0RNA
ReactionCard
1.0Thumb+1.0RNA ↔ 1.0Thumb+1.0RNA
1.0Cytidine triphosphate + 1.0RNA ↔ 1.0Pyrophosphate + 1.0RNA
ReactionCard
1.0Thumb+1.0RNA ↔ 1.0Thumb+1.0RNA
1.0Uridine triphosphate + 1.0RNA ↔ 1.0Pyrophosphate + 1.0RNA
ReactionCard
1.0Nucleoside triphosphate+1.0RNA ↔ 1.0Thumb
1.0Nucleoside triphosphate + 1.0RNA ↔ 1.0Pyrophosphate
ReactionCard
Complex Reactions:
1.0Nucleoside triphosphate+1.0RNA(n) → 1.0Thumb+1.0RNA(n+1)
1.0Nucleoside triphosphate + 1.0RNA(n) → 1.0Pyrophosphate + 1.0RNA(n+1)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB00082Cytidine triphosphateMetaboCard
ECMDB01273Guanosine triphosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB00285Uridine triphosphateMetaboCard
GO Classification:
Function
binding
catalytic activity
DNA binding
DNA-directed RNA polymerase activity
nucleic acid binding
nucleotidyltransferase activity
protein binding
protein dimerization activity
RNA polymerase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
biosynthetic process
cellular macromolecule biosynthetic process
macromolecule biosynthetic process
metabolic process
transcription
transcription, DNA-dependent
Gene Properties
Blattner:b3295
Gene OrientationCounterclockwise
Centisome Percentage:74.10
Left Sequence End3438062
Right Sequence End3439051
Gene Sequence:
>990 bp
ATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATT
ACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGC
GTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCC
AGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGACTTT
ACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACC
AATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCATT
AACGTCAGTGTCGACAGTCTGGACGCCCGCCAGTTTCACGCTATTACCGGGCAGGATAAA
TTCAACCAGGTCATGGCAGGGATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAA
GTCAATACCGTGCTGATGCGTGATGTTAATCATCACCAGCTCGACACCTTTCTGAACTGG
ATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGC
GAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGC
GGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCA
GATTACGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGC
AACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGC
GTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATT
TCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATT
ACGCAAAACTTATCGTACATTGGCGGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:329
Protein Molecular Weight:36511
Protein Theoretical pI:5
PDB File:1BDF
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>DNA-directed RNA polymerase subunit alpha
MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
IDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTLNKSGIGPVTAADITHDGD
VEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIHSEEDERPIGRLLVDACYSPV
ERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIRRAATILAEQLEAFVDLRDVRQP
EVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSL
TEIKDVLASRGLSLGMRLENWPPASIADE
References
External Links:
ResourceLink
Uniprot ID:P0A7Z4
Uniprot Name:RPOA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651354
PDB ID:1BDF
Ecogene ID:EG10893
Ecocyc:EG10893
ColiBase:b3295
Kegg Gene:b3295
EchoBASE ID:EB0886
CCDB:RPOA_ECOLI
BacMap:16131174
General Reference:
  • Bedwell, D., Davis, G., Gosink, M., Post, L., Nomura, M., Kestler, H., Zengel, J. M., Lindahl, L. (1985). "Nucleotide sequence of the alpha ribosomal protein operon of Escherichia coli." Nucleic Acids Res 13:3891-3903. Pubmed: 2989779
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Igarashi, K., Fujita, N., Ishihama, A. (1990). "Sequence analysis of two temperature-sensitive mutations in the alpha subunit gene (rpoA) of Escherichia coli RNA polymerase." Nucleic Acids Res 18:5945-5948. Pubmed: 2235479
  • Jeon, Y. H., Negishi, T., Shirakawa, M., Yamazaki, T., Fujita, N., Ishihama, A., Kyogoku, Y. (1995). "Solution structure of the activator contact domain of the RNA polymerase alpha subunit." Science 270:1495-1497. Pubmed: 7491496
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Meek, D. W., Hayward, R. S. (1984). "Nucleotide sequence of the rpoA-rplQ DNA of Escherichia coli: a second regulatory binding site for protein S4?" Nucleic Acids Res 12:5813-5821. Pubmed: 6379605
  • Ovchinnikov, Y. A., Lipkin, V. M., Modyanov, N. N., Chertov, O. Y., Smirnov, Y. V. (1977). "Primary structure of alpha-subunit of DNA-dependent RNA polymerase from Escherichia coli." FEBS Lett 76:108-111. Pubmed: 323055
  • Post, L. E., Nomura, M. (1979). "Nucleotide sequence of the intercistronic region preceding the gene for RNA polymerase subunit alpha in Escherichia coli." J Biol Chem 254:10604-10606. Pubmed: 387752
  • Schnier, J., Isono, S., Cumberlidge, A. G., Isono, K. (1985). "Unstable mutations caused by regional tandem multiplications in the gene for ribosomal protein S4 show thermosensitivity in Escherichia coli." Mol Gen Genet 199:265-270. Pubmed: 3894886
  • Zhang, G., Darst, S. A. (1998). "Structure of the Escherichia coli RNA polymerase alpha subunit amino-terminal domain." Science 281:262-266. Pubmed: 9657722