| Identification |
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| Name: | Ribonuclease 3 |
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| Synonyms: | Not Available |
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| Gene Name: | rnc |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | tRNA processing |
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| Specific Function: | Digests double-stranded RNA formed within single-strand substrates, but not RNA-DNA hybrids. Involved in the processing of rRNA precursors, viral transcripts, some mRNAs and at least 1 tRNA (metY, a minor form of tRNA-init-Met). Cleaves the 30S primary rRNA transcript to yield the immediate precursors to the 16S and 23S rRNAs; cleavage can occur in assembled 30S, 50S and even 70S subunits and is influenced by the presence of ribosomal proteins. The E.coli enzyme does not cleave R.capsulatus rRNA precursor, although R.capsulatus will complement an E.coli disruption, showing substrate recognition is different. Removes the intervening sequences from Salmonella typhimurium rRNA precursor. Complements the pre-crRNA processing defect in an rnc deletion in S.pyogenes strain 370, although this E.coli strain does not have the corresponding CRISPR locus (strain TOP10) (PubMed:23535272). |
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| Cellular Location: | Not Available |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | Not Available |
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| Metabolites: | |
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| GO Classification: | | Function |
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| ATP binding | | cytoplasm | | double-stranded RNA binding | | metal ion binding | | mRNA processing | | ribonuclease III activity | | RNA phosphodiester bond hydrolysis, endonucleolytic | | RNA processing | | rRNA binding | | rRNA catabolic process | | rRNA processing | | tRNA processing |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >681
atgaaccccatcgtaattaatcggcttcaacggaagctgggctacacttttaatcatcag
gaactgttgcagcaggcattaactcatcgtagtgccagcagtaaacataacgagcgttta
gaatttttaggcgactctattctgagctacgttatcgccaatgcgctttatcaccgtttc
cctcgtgtggatgaaggcgatatgagccggatgcgcgccacgctggtccgtggcaatacg
ctggcggaactggcgcgcgaatttgagttaggcgagtgcttacgtttagggccaggtgaa
cttaaaagcggtggatttcgtcgtgagtcaattctcgccgacaccgtcgaagcattaatt
ggtggcgtattcctcgacagtgatattcaaaccgtcgagaaattaatcctcaactggtat
caaactcgtttggacgaaattagcccaggcgataaacaaaaagatccgaaaacgcgcttg
caagaatatttgcagggtcgccatctgccgctgccgacttatctggtagtccaggtacgt
ggcgaagcgcacgatcaggaatttactatccactgccaggtcagcggcctgagtgaaccg
gtggttggcacaggttcaagccgtcgtaaggctgagcaggctgccgccgaacaggcgttg
aaaaaactggagctggaatga |
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| Protein Properties |
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| Pfam Domain Function: | Not Available |
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| Protein Residues: | 226 |
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| Protein Molecular Weight: | 25549 |
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| Protein Theoretical pI: | Not Available |
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| Signaling Regions: | Not Available |
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| Transmembrane Regions: | Not Available |
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| Protein Sequence: | >Ribonuclease 3
MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLGDSILSYVIANALYHRF
PRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELKSGGFRRESILADTVEALI
GGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRLQEYLQGRHLPLPTYLVVQVR
GEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQALKKLELE |
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| References |
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| External Links: | |
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| General Reference: | Not Available |
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