ECMDB

Identification

Name:

30S ribosomal protein S4

Synonyms:

Not Available

Gene Name:

rpsD

Enzyme Class:

Not Available

Biological Properties

General Function:

translation

Specific Function:

One of two assembly initiator proteins for the 30S subunit, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.With S5 and S12 plays an important role in translational accuracy; many suppressors of streptomycin-dependent mutants of protein S12 are found in this protein, some but not all of which decrease translational accuracy (ram, ribosomal ambiguity mutations).Plays a role in mRNA unwinding by the ribosome, possibly by forming part of a processivity clamp.Protein S4 is also a translational repressor protein, it controls the translation of the alpha-operon (which codes for S13, S11, S4, RNA polymerase alpha subunit, and L17) by binding to its mRNA.Also functions as a rho-dependent antiterminator of rRNA transcription, increasing the synthesis of rRNA under conditions of excess protein, allowing a more rapid return to homeostasis. Binds directly to RNA polymerase.

Cellular Location:

Not Available

SMPDB Pathways:

Not Available

KEGG Pathways:

Ribosome eco03010

Metabolites:

ECMDB ID	Name	View

GO Classification:

Function
cytosolic small ribosomal subunit
DNA-templated transcription, termination
mRNA 5'-UTR binding
negative regulation of translational initiation
positive regulation of translational fidelity
response to antibiotic
rRNA binding
structural constituent of ribosome
transcription antitermination
translation
translation repressor activity, nucleic acid binding

Gene Properties

Blattner:

Not Available

Gene Orientation

Not Available

Centisome Percentage:

Not Available

Left Sequence End

Not Available

Right Sequence End

Not Available

Gene Sequence:

>621
atggcaagatatttgggtcctaagctcaagctgagccgtcgtgagggcaccgacttattc
cttaagtctggcgttcgcgcgatcgataccaagtgtaaaattgaacaagctcctggccag
cacggtgcgcgtaaaccgcgtctgtctgactatggtgtgcagttgcgtgaaaagcaaaaa
gttcgccgtatctatggtgtgctggagcgtcagttccgtaactactacaaagaagcagca
cgtctgaaaggcaacaccggtgaaaacctgttggctctgctggaaggtcgtctggacaac
gttgtataccgtatgggcttcggtgccactcgtgcagaagcacgtcagctggttagccat
aaagcaattatggtaaacggtcgtgttgttaacatcgcttcttatcaggttagtccgaat
gacgttgtaagcattcgtgagaaagcgaagaagcagtctcgcgtgaaagccgctctggag
ctggctgagcagcgtgaaaagccaacctggctggaagttgatgctggcaagatggaaggt
acgtttaagcgtaagccggagcgttctgatctgtctgcggacattaacgaacacctgatc
gtcgagctttactccaagtaa

Protein Properties

Pfam Domain Function:

Not Available

Protein Residues:

206

Protein Molecular Weight:

23468

Protein Theoretical pI:

Not Available

PDB File:

1EG0

Signaling Regions:

Not Available

Transmembrane Regions:

Not Available

Protein Sequence:

>30S ribosomal protein S4
MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQK
VRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSH
KAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALELAEQREKPTWLEVDAGKMEG
TFKRKPERSDLSADINEHLIVELYSK

References

External Links:

Resource	Link
Uniprot ID:	P0A7V8
Uniprot Name:	RS4_ECOLI
PDB ID:	1EG0
CCDB:	RS4_ECOLI

General Reference:

Not Available

30S ribosomal protein S4 (P0A7V8)