Diaminopimelate epimerase (P0A6K1)

Identification
Name:Diaminopimelate epimerase
Synonyms:
  • DAP epimerase
Gene Name:dapF
Enzyme Class:
Biological Properties
General Function:Involved in diaminopimelate epimerase activity
Specific Function:LL-2,6-diaminoheptanedioate = meso- diaminoheptanedioate
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb
1.0Diaminopimelic acid ↔ 1.0Meso-2,6-Diaminoheptanedioate
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0meso-diaminopimelate
1.0Diaminopimelic acid ↔ 1.0meso-diaminopimelate
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb
1.0Diaminopimelic acid → 1.0Meso-2,6-Diaminoheptanedioate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01370Diaminopimelic acidMetaboCard
ECMDB21665Meso-2,6-DiaminoheptanedioateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
amino-acid racemase activity
catalytic activity
diaminopimelate epimerase activity
isomerase activity
racemase and epimerase activity
racemase and epimerase activity, acting on amino acids and derivatives
Process
aspartate family amino acid metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
lysine biosynthetic process
lysine biosynthetic process via diaminopimelate
lysine metabolic process
metabolic process
Gene Properties
Blattner:b3809
Gene OrientationClockwise
Centisome Percentage:86.06
Left Sequence End3992785
Right Sequence End3993609
Gene Sequence:
>825 bp
GTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCG
CAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAA
CATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG
TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCAT
TTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTG
CCGGATACCAACGAACTGGCAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGC
TGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGG
CGGACAGTGGGTTGGCTGCGAATGTTAAAACGTCTGCGCAAAGCGCGGGAACCACGGCCT
TTACGCTTAAGTCCATTGCATATGGATGTCCACGCCGGAAATTTAGTGCATAGCGCGTCA
GGGTTAAAACTCATCGACTGGGAGTATGCCGGAGATGGTGATATCGCGCTGGAACTGGCG
GCGGTGTGGGTGGAAAATACTGAACAGCACCGGCAATTGGTCAATGACTATGCCACTCGC
GCGAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTG
ATGCTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATC
AGGCTGGCCGATGACACCTGGCGGCAGCTATTAATAAAACAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:274
Protein Molecular Weight:30208
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Diaminopimelate epimerase
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHY
RIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANGRMVLTVTDDDLVRVNMGE
PNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHCVIQVDDVDTAAVETLGPVLE
SHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRV
ELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL
References
External Links:
ResourceLink
Uniprot ID:P0A6K1
Uniprot Name:DAPF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062670
Ecogene ID:EG10209
Ecocyc:EG10209
ColiBase:b3809
Kegg Gene:b3809
EchoBASE ID:EB0205
CCDB:DAPF_ECOLI
BacMap:90111650
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Colloms, S. D., Sykora, P., Szatmari, G., Sherratt, D. J. (1990). "Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases." J Bacteriol 172:6973-6980. Pubmed: 2254268
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Richaud, C., Printz, C. (1988). "Nucleotide sequence of the dapF gene and flanking regions from Escherichia coli K12." Nucleic Acids Res 16:10367. Pubmed: 3057443
  • Trotot, P., Sismeiro, O., Vivares, C., Glaser, P., Bresson-Roy, A., Danchin, A. (1996). "Comparative analysis of the cya locus in enterobacteria and related gram-negative facultative anaerobes." Biochimie 78:277-287. Pubmed: 8874804