UDP-glucose 4-epimerase (P09147)

Identification
Name:UDP-glucose 4-epimerase
Synonyms:
  • Galactowaldenase
  • UDP-galactose 4-epimerase
Gene Name:galE
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:UDP-glucose = UDP-galactose
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb
1.0UDP-Glucose ↔ 1.0Uridine diphosphategalactose
ReactionCard
1.0Thumb ↔ 1.0Thumb
1.0dTDP-D-Glucose ↔ 1.0dTDP-D-Galactose
ReactionCard
SMPDB Reactions:
1.0Thumb ↔ 1.0Uridine diphosphategalactose+1.0Thumb
1.0UDP-Glucose ↔ 1.0Uridine diphosphategalactose + 1.0Uridine diphosphategalactose
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb
1.0UDP-Glucose ↔ 1.0Uridine diphosphategalactose
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb
1.0UDP-Glucose → 1.0Uridine diphosphategalactose
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB06876dTDP-D-GalactoseMetaboCard
ECMDB01328dTDP-D-GlucoseMetaboCard
ECMDB04171UDP-GlucoseMetaboCard
ECMDB00302Uridine diphosphategalactoseMetaboCard
GO Classification:
Function
binding
catalytic activity
coenzyme binding
cofactor binding
isomerase activity
racemase and epimerase activity
racemase and epimerase activity, acting on carbohydrates and derivatives
UDP-glucose 4-epimerase activity
Process
alcohol metabolic process
cellular metabolic process
galactose metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
small molecule metabolic process
Gene Properties
Blattner:b0759
Gene OrientationCounterclockwise
Centisome Percentage:17.03
Left Sequence End790262
Right Sequence End791278
Gene Sequence:
>1017 bp
ATGAGAGTTCTGGTTACCGGTGGTAGCGGTTACATTGGAAGTCATACCTGTGTGCAATTA
CTGCAAAACGGTCATGATGTCATCATTCTTGATAACCTCTGTAACAGTAAGCGCAGCGTA
CTGCCTGTTATCGAGCGTTTAGGCGGCAAACATCCAACGTTTGTTGAAGGCGATATTCGT
AACGAAGCGTTGATGACCGAGATCCTGCACGATCACGCTATCGACACCGTGATCCACTTC
GCCGGGCTGAAAGCCGTGGGCGAATCGGTACAAAAACCGCTGGAATATTACGACAACAAT
GTCAACGGCACTCTGCGCCTGATTAGCGCCATGCGCGCCGCTAACGTCAAAAACTTTATT
TTTAGCTCCTCCGCCACCGTTTATGGCGATCAGCCCAAAATTCCATACGTTGAAAGCTTC
CCGACCGGCACACCGCAAAGCCCTTACGGCAAAAGCAAGCTGATGGTGGAACAGATCCTC
ACCGATCTGCAAAAAGCCCAGCCGGACTGGAGCATTGCCCTGCTGCGCTACTTCAACCCG
GTTGGCGCGCATCCGTCGGGCGATATGGGCGAAGATCCGCAAGGCATTCCGAATAACCTG
ATGCCATACATCGCCCAGGTTGCTGTAGGCCGTCGCGACTCGCTGGCGATTTTTGGTAAC
GATTATCCGACCGAAGATGGTACTGGCGTACGCGATTACATCCACGTAATGGATCTGGCG
GACGGTCACGTCGTGGCGATGGAAAAACTGGCGAACAAGCCAGGCGTACACATCTACAAC
CTCGGCGCTGGCGTAGGCAACAGCGTGCTGGACGTGGTTAATGCCTTCAGCAAAGCCTGC
GGCAAACCGGTTAATTATCATTTTGCACCGCGTCGCGAGGGCGACCTTCCGGCCTACTGG
GCGGACGCCAGCAAAGCCGACCGTGAACTGAACTGGCGCGTAACGCGCACACTCGATGAA
ATGGCGCAGGACACCTGGCACTGGCAGTCACGCCATCCACAGGGATATCCCGATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:338
Protein Molecular Weight:37265
Protein Theoretical pI:6
PDB File:2UDP
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>UDP-glucose 4-epimerase
MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVIERLGGKHPTFVEGDIR
NEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVNGTLRLISAMRAANVKNFI
FSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQILTDLQKAQPDWSIALLRYFNP
VGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIFGNDYPTEDGTGVRDYIHVMDLA
DGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAFSKACGKPVNYHFAPRREGDLPAYW
ADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQGYPD
References
External Links:
ResourceLink
Uniprot ID:P09147
Uniprot Name:GALE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651344
PDB ID:2UDP
Ecogene ID:EG10362
Ecocyc:EG10362
ColiBase:b0759
Kegg Gene:b0759
EchoBASE ID:EB0357
CCDB:GALE_ECOLI
BacMap:49176045
General Reference:
  • Bauer, A. J., Rayment, I., Frey, P. A., Holden, H. M. (1992). "The molecular structure of UDP-galactose 4-epimerase from Escherichia coli determined at 2.5 A resolution." Proteins 12:372-381. Pubmed: 1579570
  • Bernardi, F., Bernardi, A. (1990). "Completed sequence of pKG1800, a vector for determination of transcription terminators." DNA Seq 1:147-150. Pubmed: 2134186
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Busby, S., Dreyfus, M. (1983). "Segment-specific mutagenesis of the regulatory region in the Escherichia coli galactose operon: isolation of mutations reducing the initiation of transcription and translation." Gene 21:121-131. Pubmed: 6301942
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lemaire, H. G., Muller-Hill, B. (1986). "Nucleotide sequences of the gal E gene and the gal T gene of E. coli." Nucleic Acids Res 14:7705-7711. Pubmed: 3022232
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Thoden, J. B., Frey, P. A., Holden, H. M. (1996). "Crystal structures of the oxidized and reduced forms of UDP-galactose 4-epimerase isolated from Escherichia coli." Biochemistry 35:2557-2566. Pubmed: 8611559
  • Thoden, J. B., Frey, P. A., Holden, H. M. (1996). "High-resolution X-ray structure of UDP-galactose 4-epimerase complexed with UDP-phenol." Protein Sci 5:2149-2161. Pubmed: 8931134
  • Thoden, J. B., Gulick, A. M., Holden, H. M. (1997). "Molecular structures of the S124A, S124T, and S124V site-directed mutants of UDP-galactose 4-epimerase from Escherichia coli." Biochemistry 36:10685-10695. Pubmed: 9271499
  • Thoden, J. B., Hegeman, A. D., Wesenberg, G., Chapeau, M. C., Frey, P. A., Holden, H. M. (1997). "Structural analysis of UDP-sugar binding to UDP-galactose 4-epimerase from Escherichia coli." Biochemistry 36:6294-6304. Pubmed: 9174344
  • Thoden, J. B., Holden, H. M. (1998). "Dramatic differences in the binding of UDP-galactose and UDP-glucose to UDP-galactose 4-epimerase from Escherichia coli." Biochemistry 37:11469-11477. Pubmed: 9708982
  • Walkenhorst, H. M., Hemschemeier, S. K., Eichenlaub, R. (1995). "Molecular analysis of the molybdate uptake operon, modABCD, of Escherichia coli and modR, a regulatory gene." Microbiol Res 150:347-361. Pubmed: 8564363