Uroporphyrinogen-III synthase (P09126)

Identification
Name:Uroporphyrinogen-III synthase
Synonyms:
  • UROS
  • Hydroxymethylbilane hydrolyase [cyclizing]
  • Uroporphyrinogen-III cosynthase
Gene Name:hemD
Enzyme Class:
Biological Properties
General Function:Involved in uroporphyrinogen-III synthase activity
Specific Function:Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Hydroxymethylbilane ↔ 1.0Water + 1.0Uroporphyrinogen III
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Hydroxymethylbilane ↔ 1.0Water + 1.0Uroporphyrinogen III
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydroxymethylbilane → 1.0Uroporphyrinogen III + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01137HydroxymethylbilaneMetaboCard
ECMDB01086Uroporphyrinogen IIIMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
carbon-oxygen lyase activity
catalytic activity
hydro-lyase activity
lyase activity
uroporphyrinogen-III synthase activity
Process
biosynthetic process
cellular biosynthetic process
heterocycle biosynthetic process
metabolic process
tetrapyrrole biosynthetic process
Gene Properties
Blattner:b3804
Gene OrientationCounterclockwise
Centisome Percentage:85.94
Left Sequence End3987111
Right Sequence End3987851
Gene Sequence:
>741 bp
ATGAGTATCCTTGTCACCCGCCCGTCTCCCGCTGGAGAAGAGTTAGTGAGCCGTCTGCGC
ACACTGGGGCAGGTGGCCTGGCATTTTCCGCTGATTGAGTTTTCTCCGGGTCAACAATTA
CCGCAACTTGCTGATCAACTGGCAGCGCTGGGGGAGAGCGATCTGTTGTTTGCCCTCTCG
CAACACGCGGTTGCTTTTGCCCAATCACAGCTGCATCAGCAAGATCGTAAATGGCCCCGA
CTACCTGATTATTTCGCCATTGGACGCACCACCGCACTGGCACTACATACCGTAAGTGGA
CAGAAGATTCTCTACCCGCAGGATCGGGAAATCAGCGAAGTCTTGCTACAATTACCTGAA
TTACAAAATATTGCGGGCAAACGTGCGCTGATATTACGTGGCAATGGTGGTCGTGAGCTA
ATTGGGGATACCCTGACGGCGCGCGGTGCTGAGGTCACTTTTTGTGAATGTTATCAACGA
TGCGCAATCCATTACGATGGTGCAGAAGAAGCGATGCGCTGGCAAGCCCGCGAGGTGACG
ATGGTCGTTGTTACCAGCGGTGAAATGTTGCAGCAACTCTGGTCGCTGATCCCACAATGG
TATCGTGAGCACTGGTTACTACACTGTCGACTATTGGTCGTCAGTGAGCGTTTGGCGAAA
CTCGCCCGGGAACTGGGCTGGCAAGACATTAAGGTCGCCGATAACGCTGACAACGATGCG
CTTTTACGGGCATTACAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:246
Protein Molecular Weight:27798
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Uroporphyrinogen-III synthase
MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLADQLAALGESDLLFALS
QHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQKILYPQDREISEVLLQLPE
LQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQRCAIHYDGAEEAMRWQAREVT
MVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERLAKLARELGWQDIKVADNADNDA
LLRALQ
References
External Links:
ResourceLink
Uniprot ID:P09126
Uniprot Name:HEM4_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676247
Ecogene ID:EG10430
Ecocyc:EG10430
ColiBase:b3804
Kegg Gene:b3804
EchoBASE ID:EB0425
CCDB:HEM4_ECOLI
BacMap:16131656
General Reference:
  • Alefounder, P. R., Abell, C., Battersby, A. R. (1988). "The sequence of hemC, hemD and two additional E. coli genes." Nucleic Acids Res 16:9871. Pubmed: 3054815
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jordan, P. M., Mgbeje, B. I., Alwan, A. F., Thomas, S. D. (1987). "Nucleotide sequence of hemD, the second gene in the hem operon of Escherichia coli K-12." Nucleic Acids Res 15:10583. Pubmed: 3320969
  • Jordan, P. M., Mgbeje, B. I., Thomas, S. D., Alwan, A. F. (1988). "Nucleotide sequence for the hemD gene of Escherichia coli encoding uroporphyrinogen III synthase and initial evidence for a hem operon." Biochem J 249:613-616. Pubmed: 3277628
  • Sasarman, A., Nepveu, A., Echelard, Y., Dymetryszyn, J., Drolet, M., Goyer, C. (1987). "Molecular cloning and sequencing of the hemD gene of Escherichia coli K-12 and preliminary data on the Uro operon." J Bacteriol 169:4257-4262. Pubmed: 3040684