Identification
Name:L-ribulose-5-phosphate 4-epimerase
Synonyms:
  • Phosphoribulose isomerase
Gene Name:araD
Enzyme Class:
Biological Properties
General Function:Involved in metal ion binding
Specific Function:L-ribulose 5-phosphate = D-xylulose 5- phosphate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Ascorbate and aldarate metabolism ec00053
  • Metabolic pathways eco01100
  • Microbial metabolism in diverse environments ec01120
  • Pentose and glucuronate interconversions ec00040
KEGG Reactions:
1.0Thumb1.0Thumb
EcoCyc Reactions:
1.0Thumb1.0Thumb
Complex Reactions:
1.0Thumb1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB20167L-Ribulose 5-phosphateMetaboCard
ECMDB04178Xylulose 5-phosphateMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
ion binding
isomerase activity
L-ribulose-phosphate 4-epimerase activity
metal ion binding
racemase and epimerase activity
racemase and epimerase activity, acting on carbohydrates and derivatives
transition metal ion binding
zinc ion binding
Process
alcohol metabolic process
arabinose catabolic process
L-arabinose catabolic process
metabolic process
monosaccharide metabolic process
pentose catabolic process
pentose metabolic process
small molecule metabolic process
Gene Properties
Blattner:b0061
Gene OrientationCounterclockwise
Centisome Percentage:1.42
Left Sequence End65855
Right Sequence End66550
Gene Sequence:
>696 bp
ATGTTAGAAGATCTCAAACGCCAGGTATTAGAAGCCAACCTGGCGCTGCCAAAACACAAC
CTGGTCACGCTCACATGGGGCAACGTCAGCGCCGTTGATCGCGAGCGCGGCGTCTTTGTG
ATCAAACCTTCCGGCGTCGATTACAGCGTCATGACCGCTGACGATATGGTCGTGGTTAGC
ATCGAAACCGGTGAAGTGGTTGAAGGTACGAAAAAGCCCTCCTCCGACACGCCAACTCAC
CGGCTGCTCTATCAGGCATTCCCCTCCATTGGCGGCATTGTGCATACGCACTCGCGCCAC
GCCACCATCTGGGCGCAGGCGGGTCAGTCGATTCCAGCAACCGGCACCACCCACGCCGAC
TATTTCTACGGCACCATTCCCTGCACCCGCAAAATGACCGACGCAGAAATCAACGGCGAA
TATGAGTGGGAAACCGGTAACGTCATCGTAGAAACCTTTGAAAAACAGGGTATCGATGCA
GCGCAAATGCCCGGCGTTCTGGTCCATTCCCACGGCCCGTTTGCATGGGGCAAAAATGCC
GAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGCTTATATGGGGATATTCTGC
CGTCAGTTAGCGCCGCAGTTACCGGATATGCAGCAAACGCTGCTGGATAAACACTATCTG
CGTAAGCATGGCGCGAAGGCATATTACGGGCAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:231
Protein Molecular Weight:25519
Protein Theoretical pI:6
PDB File:1JDI
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>L-ribulose-5-phosphate 4-epimerase
MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPSGVDYSVMTADDMVVVS
IETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHATIWAQAGQSIPATGTTHAD
YFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDAAQMPGVLVHSHGPFAWGKNA
EDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKHYLRKHGAKAYYGQ
References
External Links:
ResourceLink
Uniprot ID:P08203
Uniprot Name:ARAD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321943
PDB ID:1JDI
Ecogene ID:EG10055
Ecocyc:EG10055
ColiBase:b0061
Kegg Gene:b0061
EchoBASE ID:EB0053
CCDB:ARAD_ECOLI
BacMap:16128055
General Reference:
  • Andersson, A., Schneider, G., Lindqvist, Y. (1995). "Purification and preliminary X-ray crystallographic studies of recombinant L-ribulose-5-phosphate 4-epimerase from Escherichia coli." Protein Sci 4:1648-1650. Pubmed: 8520491
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bonner, C. A., Hays, S., McEntee, K., Goodman, M. F. (1990). "DNA polymerase II is encoded by the DNA damage-inducible dinA gene of Escherichia coli." Proc Natl Acad Sci U S A 87:7663-7667. Pubmed: 2217198
  • Chen, H., Sun, Y., Stark, T., Beattie, W., Moses, R. E. (1990). "Nucleotide sequence and deletion analysis of the polB gene of Escherichia coli." DNA Cell Biol 9:631-635. Pubmed: 2261080
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Iwasaki, H., Ishino, Y., Toh, H., Nakata, A., Shinagawa, H. (1991). "Escherichia coli DNA polymerase II is homologous to alpha-like DNA polymerases." Mol Gen Genet 226:24-33. Pubmed: 2034216
  • Lee, N., Gielow, W., Martin, R., Hamilton, E., Fowler, A. (1986). "The organization of the araBAD operon of Escherichia coli." Gene 47:231-244. Pubmed: 3549454
  • Lee, N., Patrick, J. W., Masson, M. (1968). "Crystalline L-ribulose 5-phosphate 4-epimerase from Escherichia coli." J Biol Chem 243:4700-4705. Pubmed: 4879898
  • Luo, Y., Samuel, J., Mosimann, S. C., Lee, J. E., Tanner, M. E., Strynadka, N. C. (2001). "The structure of L-ribulose-5-phosphate 4-epimerase: an aldolase-like platform for epimerization." Biochemistry 40:14763-14771. Pubmed: 11732895
  • Mineno, J., Fukui, H., Ishino, Y., Kato, I., Shinagawa, H. (1990). "Nucleotide sequence of the araD gene of Escherichia coli K12 encoding the L-ribulose 5-phosphate 4-epimerase." Nucleic Acids Res 18:6722. Pubmed: 2251150
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901