1,4-alpha-glucan-branching enzyme (P07762)

Identification
Name:1,4-alpha-glucan-branching enzyme
Synonyms:
  • 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
  • Glycogen-branching enzyme
  • BE
Gene Name:glgB
Enzyme Class:
Biological Properties
General Function:Involved in 1,4-alpha-glucan branching enzyme activity
Specific Function:Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. Has a preference for transferring chains of 5 to 16 glucose units
Cellular Location:Cytoplasmic
SMPDB Pathways:
  • Secondary metabolites: Trehalose Biosynthesis and Metabolism PW000968
  • Starch and sucrose metabolism PW000941
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Starch
1.01,4-alpha-D-glucan ↔ 1.0Starch
ReactionCard
SMPDB Reactions:
1.0Thumb ↔ 1.0Thumb
1.0Amylose ↔ 1.0Glycogen
ReactionCard
EcoCyc Reactions:
1.0a 1,4-α-D-glucan ↔ 1.0Thumb
1.0a 1,4-α-D-glucan ↔ 1.0Glycogen
ReactionCard
Complex Reactions:
1.0branching glycogen → 1.0Thumb
1.0branching glycogen → 1.0Glycogen
ReactionCard
1.0Thumb → 1.0branching glycogen
1.0Glycogen → 1.0branching glycogen
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211101,4-alpha-D-glucanMetaboCard
ECMDB24082AmyloseMetaboCard
ECMDB21341GlycogenMetaboCard
GO Classification:
Function
1,4-alpha-glucan branching enzyme activity
binding
catalytic activity
cation binding
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing O-glycosyl compounds
ion binding
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
Process
alcohol metabolic process
carbohydrate metabolic process
glucose metabolic process
glycogen biosynthetic process
glycogen metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
primary metabolic process
small molecule metabolic process
Gene Properties
Blattner:b3432
Gene OrientationCounterclockwise
Centisome Percentage:76.93
Left Sequence End3569339
Right Sequence End3571525
Gene Sequence:
>2187 bp
ATGTCCGATCGTATCGATAGAGACGTGATTAACGCGCTAATTGCAGGCCATTTTGCGGAT
CCTTTTTCCGTACTGGGAATGCATAAAACCACCGCGGGACTGGAAGTCCGTGCCCTTTTA
CCCGACGCTACCGATGTGTGGGTGATTGAACCGAAAACCGGGCGCAAACTCGCAAAACTG
GAGTGTCTCGACTCACGGGGATTCTTTAGCGGCGTCATTCCGCGACGTAAGAATTTTTTC
CGCTATCAGTTGGCTGTTGTCTGGCATGGTCAGCAAAACCTGATTGATGATCCTTACCGT
TTTGGTCCGCTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTGCGC
CCGTATGAAACCTTAGGCGCGCATGCAGATACTATGGATGGCGTCACAGGTACGCGTTTC
TCTGTCTGGGCTCCAAACGCCCGTCGGGTCTCGGTGGTTGGGCAATTCAACTACTGGGAC
GGTCGCCGTCACCCGATGCGCCTGCGTAAAGAGAGCGGCATCTGGGAACTGTTTATCCCT
GGGGCGCATAACGGTCAGCTCTATAAATACGAGATGATTGATGCCAATGGCAACTTGCGT
CTGAAGTCCGACCCTTATGCCTTTGAAGCGCAAATGCGCCCGGAAACCGCGTCTCTTATT
TGCGGGCTGCCGGAAAAGGTTGTACAGACTGAAGAGCGCAAAAAAGCGAATCAGTTTGAT
GCGCCAATCTCTATTTATGAAGTTCACCTGGGTTCCTGGCGTCGCCACACCGACAACAAT
TTCTGGTTGAGCTACCGCGAGCTGGCCGATCAACTGGTGCCTTATGCTAAATGGATGGGC
TTTACCCACCTCGAACTACTGCCCATTAACGAGCATCCCTTCGATGGCAGTTGGGGTTAT
CAGCCAACCGGCCTGTATGCGCCAACCCGCCGTTTTGGTACTCGCGACGACTTCCGTTAT
TTCATTGATGCCGCACACGCAGCTGGTCTGAACGTGATTCTCGACTGGGTGCCAGGCCAC
TTCCCGACTGATGACTTTGCGCTTGCCGAATTTGATGGCACGAACTTGTATGAACACAGC
GATCCGCGTGAAGGCTATCATCAGGACTGGAACACGCTGATCTACAACTATGGTCGCCGT
GAAGTCAGTAACTTCCTCGTCGGTAACGCGCTTTACTGGATTGAACGTTTTGGTATTGAT
GCGCTGCGCGTCGATGCGGTGGCGTCAATGATTTATCGCGACTACAGCCGTAAAGAGGGG
GAGTGGATCCCGAACGAATTTGGCGGGCGCGAGAATCTTGAAGCGATTGAATTCTTGCGT
AATACCAACCGTATTCTTGGTGAGCAGGTTTCCGGTGCGGTGACAATGGCTGAGGAGTCT
ACCGATTTCCCTGGCGTTTCTCGTCCGCAGGATATGGGCGGTCTGGGCTTCTGGTACAAG
TGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGT
CAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACACTGAAAACTTCGTC
CTGCCGTTGTCGCATGATGAAGTGGTCCACGGTAAAAAATCGATTCTCGACCGCATGCCG
GGCGACGCATGGCAGAAATTCGCGAACCTGCGCGCCTACTATGGCTGGATGTGGGCATTC
CCGGGCAAGAAACTACTGTTCATGGGTAACGAATTTGCCCAGGGCCGCGAGTGGAACCAT
GACGCCAGCCTCGACTGGCATCTGTTGGAAGGCGGCGATAACTGGCACCACGGTGTCCAG
CGTCTGGTGCGCGATCTGAACCTCACCTACCGCCACCATAAAGCAATGCATGAACTGGAT
TTTGACCCGTACGGCTTTGAATGGCTGGTGGTGGATGACAAAGAACGCTCGGTGCTGATC
TTTGTGCGTCGCGATAAAGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACGCCGGTA
CCGCGTCATGATTATCGCTTCGGCATAAACCAGCCGGGCAAATGGCGTGAAATCCTCAAT
ACCGATTCCATGCACTATCACGGCAGTAATGCAGGCAATGGCGGCACGGTACACAGCGAT
GAGATTGCCAGCCACGGTCGTCAGCATTCACTAAGCCTGACGCTACCACCGCTGGCCACT
ATCTGGCTGGTTCGGGAGGCAGAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:728
Protein Molecular Weight:84336
Protein Theoretical pI:6
PDB File:1M7X
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>1,4-alpha-glucan-branching enzyme
MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDATDVWVIEPKTGRKLAKL
ECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFGPLIQEMDAWLLSEGTHLR
PYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWDGRRHPMRLRKESGIWELFIP
GAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETASLICGLPEKVVQTEERKKANQFD
APISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYAKWMGFTHLELLPINEHPFDGSWGY
QPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVILDWVPGHFPTDDFALAEFDGTNLYEHS
DPREGYHQDWNTLIYNYGRREVSNFLVGNALYWIERFGIDALRVDAVASMIYRDYSRKEG
EWIPNEFGGRENLEAIEFLRNTNRILGEQVSGAVTMAEESTDFPGVSRPQDMGGLGFWYK
WNLGWMHDTLDYMKLDPVYRQYHHDKLTFGILYNYTENFVLPLSHDEVVHGKKSILDRMP
GDAWQKFANLRAYYGWMWAFPGKKLLFMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQ
RLVRDLNLTYRHHKAMHELDFDPYGFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPV
PRHDYRFGINQPGKWREILNTDSMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLAT
IWLVREAE
References
External Links:
ResourceLink
Uniprot ID:P07762
Uniprot Name:GLGB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676610
PDB ID:1M7X
Ecogene ID:EG10378
Ecocyc:EG10378
ColiBase:b3432
Kegg Gene:b3432
EchoBASE ID:EB0373
CCDB:GLGB_ECOLI
BacMap:16131306
General Reference:
  • Abad, M. C., Binderup, K., Rios-Steiner, J., Arni, R. K., Preiss, J., Geiger, J. H. (2002). "The X-ray crystallographic structure of Escherichia coli branching enzyme." J Biol Chem 277:42164-42170. Pubmed: 12196524
  • Baecker, P. A., Greenberg, E., Preiss, J. (1986). "Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-alpha-D-(1, 4-alpha-D-glucano)-transferase as deduced from the nucleotide sequence of the glg B gene." J Biol Chem 261:8738-8743. Pubmed: 3013861
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553