tRNA pseudouridine synthase A (P07649)

Identification
Name:tRNA pseudouridine synthase A
Synonyms:
  • tRNA pseudouridylate synthase I
  • PSU-I
  • tRNA-uridine isomerase I
Gene Name:truA
Enzyme Class:
Biological Properties
General Function:Translation, ribosomal structure and biogenesis
Specific Function:Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
GO Classification:
Function
binding
catalytic activity
intramolecular transferase activity
isomerase activity
nucleic acid binding
pseudouridine synthase activity
RNA binding
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
pseudouridine synthesis
RNA metabolic process
RNA modification
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>813 bp
ATGTCCGACCAGCAACAACCGCCAGTTTATAAAATTGCGCTGGGCATTGAGTACGACGGC
AGTAAGTATTACGGCTGGCAACGGCAGAACGAAGTCCGCAGTGTGCAGGAGAAGCTGGAA
AAGGCGCTCTCCCAGGTGGCGAACGAACCCATCACCGTCTTCTGCGCCGGGCGTACTGAC
GCAGGGGTACACGGTACCGGGCAGGTTGTGCATTTCGAAACAACCGCGCTGCGCAAAGAC
GCGGCGTGGACCTTGGGCGTAAATGCGAATTTACCTGGTGACATCGCTGTGCGTTGGGTT
AAAACTGTACCTGATGATTTTCATGCCCGATTTAGCGCCACGGCTCGCCGTTATCGCTAC
ATCATCTACAATCATCGGCTGCGCCCGGCGGTACTGAGTAAAGGGGTAACCCATTTTTAC
GAACCGCTGGACGCTGAACGGATGCATCGGGCTGCGCAATGCTTGCTGGGCGAGAATGAT
TTCACCTCGTTCCGTGCGGTGCAGTGCCAGTCCCGAACCCCGTGGCGCAACGTTATGCAT
ATTAATGTCACGCGTCACGGTCCTTATGTGGTGGTAGATATTAAAGCGAATGCTTTTGTA
CATCATATGGTCAGGAATATTGTCGGCAGCCTGATGGAAGTCGGTGCCCACAACCAGCCG
GAGAGCTGGATAGCAGAGTTGCTGGCGGCAAAGGACAGAACGCTGGCGGCAGCAACGGCA
AAAGCGGAAGGGCTGTATCTGGTCGCGGTGGATTACCCTGACCGGTATGATCTTCCAAAA
CCGCCAATGGGCCCGCTATTTCTGGCGGACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:270
Protein Molecular Weight:30399
Protein Theoretical pI:9
PDB File:1DJ0
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>tRNA pseudouridine synthase A
MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALSQVANEPITVFCAGRTD
AGVHGTGQVVHFETTALRKDAAWTLGVNANLPGDIAVRWVKTVPDDFHARFSATARRYRY
IIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGENDFTSFRAVQCQSRTPWRNVMH
INVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHNQPESWIAELLAAKDRTLAAATA
KAEGLYLVAVDYPDRYDLPKPPMGPLFLAD
References
External Links:
ResourceLink
Uniprot ID:P07649
Uniprot Name:TRUA_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:1788657
PDB ID:1DJ0
CCDB:TRUA_ECOLI
General Reference:
  • Arps, P. J., Marvel, C. C., Rubin, B. C., Tolan, D. A., Penhoet, E. E., Winkler, M. E. (1985). "Structural features of the hisT operon of Escherichia coli K-12." Nucleic Acids Res 13:5297-5315. Pubmed: 2991861
  • Arps, P. J., Winkler, M. E. (1987). "Structural analysis of the Escherichia coli K-12 hisT operon by using a kanamycin resistance cassette." J Bacteriol 169:1061-1070. Pubmed: 3029016
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Foster, P. G., Huang, L., Santi, D. V., Stroud, R. M. (2000). "The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I." Nat Struct Biol 7:23-27. Pubmed: 10625422
  • Gu, X., Liu, Y., Santi, D. V. (1999). "The mechanism of pseudouridine synthase I as deduced from its interaction with 5-fluorouracil-tRNA." Proc Natl Acad Sci U S A 96:14270-14275. Pubmed: 10588695
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Huang, L., Pookanjanatavip, M., Gu, X., Santi, D. V. (1998). "A conserved aspartate of tRNA pseudouridine synthase is essential for activity and a probable nucleophilic catalyst." Biochemistry 37:344-351. Pubmed: 9425056
  • Kammen, H. O., Marvel, C. C., Hardy, L., Penhoet, E. E. (1988). "Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I." J Biol Chem 263:2255-2263. Pubmed: 3276686
  • Nonet, M. L., Marvel, C. C., Tolan, D. R. (1987). "The hisT-purF region of the Escherichia coli K-12 chromosome. Identification of additional genes of the hisT and purF operons." J Biol Chem 262:12209-12217. Pubmed: 3040734
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837